Product Description
LIGHT LABS is proud to offer Mediatech DMEM — Dulbecco's Modification of Eagle's Medium (Cat # 15-018-CV) — the most comprehensively supplemented yet maximally flexible DMEM formulation in the Mediatech lineup, providing 4.5 g/L high-glucose DMEM with 25 mM HEPES buffer and sodium pyruvate, but WITHOUT L-glutamine. The 15-018-CV is the premier choice for researchers who need all the performance advantages of HEPES buffering and sodium pyruvate in their DMEM baseline while retaining complete independent control over glutamine supplementation — either because they use stable dipeptide glutamine substitutes such as GlutaMAX™ or Corning® GlutaGRO™, because they are studying glutamine metabolism, or because their custom media protocol requires precise glutamine concentration control.
The unique combination of features in the 15-018-CV addresses three distinct research needs simultaneously in a single formulation. The 25 mM HEPES buffer provides superior pH stability beyond the bicarbonate system alone — critical for high-throughput workflows, extended bench manipulations, and any environment where CO₂ concentration fluctuates. The sodium pyruvate provides an additional energy substrate supporting mitochondrial metabolism and cell growth — important for cell types that benefit from pyruvate supplementation or for overcoming pyruvate-sensitive metabolic bottlenecks in cell culture. The absence of L-glutamine provides the flexibility to add glutamine at a precisely defined concentration or substitute it with a stable dipeptide alternative. Together, these three features make the 15-018-CV the most versatile single-bottle DMEM formulation for researchers with complex or custom cell culture supplementation requirements.
Like all Mediatech DMEM formulations, the 15-018-CV incorporates Pyridoxine HCl instead of Pyridoxal for improved stability, and every production lot undergoes comprehensive quality testing with a lot-specific Certificate of Analysis. Manufactured under ISO 13485 certified quality management system. LIGHT LABS is your trusted source for Mediatech cell culture media and reagents.
KEY FEATURES
- High-glucose DMEM with 25 mM HEPES + sodium pyruvate + NO L-glutamine — most versatile DMEM formulation; combines HEPES pH stability and pyruvate energy support with full glutamine supplementation flexibility
- 25 mM HEPES buffer — robust pH stability outside the CO₂ incubator; ideal for high-throughput workflows, core facilities, and extended bench manipulations; dual bicarbonate + HEPES buffer system
- Sodium pyruvate included — additional energy substrate supporting mitochondrial metabolism; important for pyruvate-sensitive cell types and metabolic optimization
- Without L-glutamine — add L-glutamine, GlutaMAX™, or Corning® GlutaGRO™ at precisely defined concentrations; essential for glutamine metabolism studies and custom supplementation protocols
- 4.5 g/L high-glucose formulation — standard high-glucose concentration for rapidly dividing mammalian cell lines
- Pyridoxine HCl substituted for Pyridoxal — improved stability over the original DMEM formulation
- Eliminates need to stock multiple DMEM variants — one formulation provides HEPES buffering, pyruvate support, AND glutamine flexibility
- Comprehensive lot-specific quality control — growth promotion, cytotoxicity, endotoxin (<0.25 EU/mL), sterility, and mycoplasma testing on every lot
- Complete Certificate of Analysis available for each production lot
- Manufactured under ISO 13485 certified quality management system
- Liquid, sterile, ready-to-use format (add glutamine before use)
- 18-month shelf life
- Storage: 2°C to 8°C
- 500 mL bottles; 6 bottles per case
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 15-018-CV |
| Brand | Mediatech |
| Product Name | DMEM — Dulbecco's Modification of Eagle's Medium |
| Formulation | High glucose (4.5 g/L); 25 mM HEPES; sodium pyruvate; WITHOUT L-glutamine |
| Glucose Concentration | 4.5 g/L |
| L-Glutamine | No — NOT included; add before use |
| Sodium Pyruvate | Yes — included |
| HEPES | Yes — 25 mM |
| Sodium Bicarbonate | Yes — included |
| Pyridoxine Source | Pyridoxine HCl (improved stability vs. Pyridoxal) |
| Format | Liquid, sterile, ready-to-use (add glutamine before use) |
| Endotoxin Level | <0.25 EU/mL (LAL chromogenic assay) |
| Sterility Testing | Yes — each lot tested |
| Mycoplasma Testing | Yes — each lot tested |
| Quality System | ISO 13485 certified |
| Certificate of Analysis | Yes — lot-specific CoA available |
| Shelf Life | 18 months |
| Volume | 500 mL per bottle |
| Packaging | 6 bottles per case |
| Storage Temperature | 2°C to 8°C |
COMPLETE MEDIATECH DMEM LINEUP — HEPES, PYRUVATE & GLUTAMINE OPTIONS
| Cat # | Glucose | L-Gln | Na Pyruvate | HEPES | Best For |
|---|---|---|---|---|---|
| 15-018-CV | 4.5 g/L | No | Yes | 25 mM | Maximum flexibility: HEPES + pyruvate + custom glutamine |
| 10-013-CV | 4.5 g/L | Yes | Yes | No | Most routine cell culture |
| 10-017-CV | 4.5 g/L | Yes | No | No | Standard; add pyruvate separately |
| 15-017-CV | 4.5 g/L | No | No | No | Glutamine-free; minimal supplements |
| 10-014-CV | 4.5 g/L | No | Yes | No | Pyruvate + no glutamine; no HEPES |
APPLICATIONS
- DMEM-based cell culture requiring maximum combined flexibility: HEPES buffering + pyruvate energy + custom glutamine control
- Glutamine metabolism studies in HEPES-buffered, pyruvate-supplemented DMEM
- GlutaMAX™ or GlutaGRO™ supplementation in HEPES + pyruvate DMEM base
- High-throughput cell culture workflows requiring stable pH and pyruvate energy support without pre-defined glutamine
- Custom media formulation requiring DMEM base with HEPES and pyruvate
- Core facility high-throughput DMEM workflows requiring stable pH during bench manipulations
- Any adherent mammalian cell culture application requiring DMEM with HEPES, sodium pyruvate, and independently controlled glutamine
ORDERING INFORMATION — MEDIATECH DMEM FORMULATIONS
| Cat # | L-Gln | Na Pyr | HEPES | Volume | Pkg |
|---|---|---|---|---|---|
| 15-018-CV | No | Yes | 25 mM | 500 mL | 6/case |
| 15-018-CM | No | Yes | 25 mM | 1 L | 6/case |
| 10-013-CV | Yes | Yes | No | 500 mL | 6/case |
| 10-017-CV | Yes | No | No | 500 mL | 6/case |
| 15-017-CV | No | No | No | 500 mL | 6/case |
| 10-014-CV | No | Yes | No | 500 mL | 6/case |
| 10-092-CV | DMEM/F-12 50/50 — L-Gln + 15 mM HEPES | Yes | No | 500 mL | 6/case |
| 10-090-CV | DMEM/F-12 50/50 — L-Gln, No HEPES | Yes | No | 500 mL | 6/case |
