Product Description
LIGHT LABS is proud to offer Mediatech IMDM — Iscove's Modification of DMEM (Cat # 10-016-CV) — one of the most highly enriched synthetic basal media available for mammalian cell culture, specifically optimized for rapidly proliferating cultures at high cell density. The 10-016-CV formulation contains L-glutamine and 25 mM HEPES buffer, and does not contain α-thioglycerol or β-mercaptoethanol — providing ready-to-use IMDM for a broad range of hematopoietic, lymphoid, hybridoma, and other rapidly dividing cell culture applications that require the enriched nutrient environment of IMDM.
Iscove's Modification of DMEM (IMDM) was developed by Neil Iscove and Frank Melchers as a highly enriched modification of Dulbecco's Modified Eagle Medium optimized for the culture of rapidly proliferating cells at high density — particularly hematopoietic precursor cells, lymphocytes, and hybridoma cells used in monoclonal antibody production. Compared to standard DMEM, IMDM contains additional and higher concentrations of amino acids and vitamins, supplemental selenium, and is formulated with potassium nitrate in place of ferric nitrate for improved iron bioavailability. Most significantly, IMDM contains 25 mM HEPES buffer in addition to sodium bicarbonate — providing superior pH stability both inside and outside the CO₂ incubator, which is critical for high-density cultures that generate CO₂ rapidly and for bench-top manipulations of the cultures.
The α-thioglycerol and β-mercaptoethanol-free formulation of the 10-016-CV provides maximum flexibility — these reducing agents are required by some cell types (particularly hematopoietic progenitors and embryonic stem cells) but inhibitory to others, and their exclusion from the basal medium allows researchers to add them at defined concentrations only where required. The addition of albumin and transferrin may be required for certain cell types — particularly for serum-free or low-serum applications. Pyridoxine HCl is substituted for Pyridoxal for improved stability. Every production lot undergoes comprehensive quality testing with a lot-specific Certificate of Analysis. Manufactured under ISO 13485 certified quality management system. LIGHT LABS is your trusted source for Mediatech cell culture media and reagents.
KEY FEATURES
- Highly enriched synthetic medium optimized for rapidly proliferating cells at high density — more amino acids, vitamins, and selenium than standard DMEM
- L-glutamine and 25 mM HEPES included — superior pH stability inside and outside the CO₂ incubator; critical for high-density cultures that generate CO₂ rapidly
- Without α-thioglycerol and β-mercaptoethanol — maximum flexibility; add reducing agents only where required by specific cell types
- Potassium nitrate replaces ferric nitrate — improved iron bioavailability vs. standard DMEM formulation
- Selenium included — essential micronutrient for high-density and serum-free culture; supports antioxidant defense in rapidly proliferating cells
- Higher amino acid and vitamin concentrations vs. DMEM — supports the metabolic demands of rapidly dividing cells at high density
- Pyridoxine HCl substituted for Pyridoxal — improved stability over the original formulation
- Note: addition of albumin and transferrin may be required for certain cell types, particularly in serum-free applications
- Comprehensive lot-specific quality control — growth promotion, cytotoxicity, endotoxin (<0.25 EU/mL), sterility, and mycoplasma testing on every lot
- Complete Certificate of Analysis available for each production lot
- Manufactured under ISO 13485 certified quality management system
- Liquid, sterile, ready-to-use format
- 12-month shelf life
- Storage: 2°C to 8°C
- 500 mL bottles; 6 bottles per case
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 10-016-CV |
| Brand | Mediatech |
| Product Name | IMDM — Iscove's Modification of DMEM |
| L-Glutamine | Yes — included |
| HEPES | Yes — 25 mM |
| α-Thioglycerol | No — NOT included |
| β-Mercaptoethanol | No — NOT included |
| Selenium | Yes — included |
| Iron Source | Potassium nitrate (replaces ferric nitrate) |
| Pyridoxine Source | Pyridoxine HCl (improved stability vs. Pyridoxal) |
| Format | Liquid, sterile, ready-to-use |
| Endotoxin Level | <0.25 EU/mL (LAL chromogenic assay) |
| Sterility Testing | Yes — each lot tested |
| Mycoplasma Testing | Yes — each lot tested |
| Quality System | ISO 13485 certified |
| Certificate of Analysis | Yes — lot-specific CoA and full formulation available |
| Shelf Life | 12 months |
| Volume | 500 mL per bottle |
| Packaging | 6 bottles per case |
| Storage Temperature | 2°C to 8°C |
IMDM vs. DMEM — KEY DIFFERENCES
| Feature | IMDM (10-016-CV) | Standard DMEM (10-013-CV) |
|---|---|---|
| Amino Acids | Additional & higher concentrations | Standard |
| Vitamins | Additional & higher concentrations | Standard |
| Selenium | Yes | No |
| Iron Source | Potassium nitrate | Ferric nitrate |
| HEPES | 25 mM | No |
| Reducing Agents | Not included (add separately) | Not applicable |
| Best For | High-density rapidly proliferating cultures; hematopoietic; hybridoma | Routine mammalian cell culture |
APPLICATIONS
- Culture of hematopoietic precursor cells and progenitor cells
- Lymphocyte and lymphoid cell line culture
- Hybridoma cell culture for monoclonal antibody production
- B-cell and T-cell culture and expansion
- High-density rapidly proliferating mammalian cell cultures
- Myeloma cell culture
- Embryonic stem cell culture (with addition of reducing agents as required)
- Serum-free or low-serum culture with albumin and transferrin supplementation
- Any application requiring a highly enriched basal medium with HEPES buffering for rapidly dividing cells at high density
ORDERING INFORMATION — MEDIATECH IMDM (ISCOVE'S MODIFICATION OF DMEM)
| Cat # | Description | Volume | Pkg |
|---|---|---|---|
| 10-016-CV | IMDM, L-Glutamine, 25 mM HEPES, No β-ME/α-TG | 500 mL | 6/case |
| 10-016-CM | IMDM, L-Glutamine, 25 mM HEPES, No β-ME/α-TG | 1 L | 6/case |
| 15-016-CV | IMDM, No L-Glutamine, 25 mM HEPES | 500 mL | 6/case |
| 10-013-CV | DMEM, High Glucose, L-Glutamine, Sodium Pyruvate | 500 mL | 6/case |
| 10-017-CV | DMEM, High Glucose, L-Glutamine, No Sodium Pyruvate | 500 mL | 6/case |
| 15-017-CV | DMEM, High Glucose, No L-Glutamine, No Sodium Pyruvate | 500 mL | 6/case |
| 10-090-CV | DMEM/Ham's F-12 50/50 Mix, L-Glutamine, No HEPES | 500 mL | 6/case |
| 10-092-CV | DMEM/Ham's F-12 50/50 Mix, L-Glutamine, 15 mM HEPES | 500 mL | 6/case |
| 21-031-CV | DPBS, 1X, Without Calcium & Magnesium | 500 mL | 6/case |
