Product Description
LIGHT LABS is proud to offer Mediatech Phosphate-Buffered Saline (PBS), 1X Without Calcium and Magnesium (Cat # 21-040-CV) — one of the most fundamental and widely used buffer solutions in cell biology, molecular biology, and biochemistry, provided in a convenient 6 x 500 mL case format. PBS is an isotonic, physiologically pH-balanced saline buffer containing sodium chloride and sodium phosphate, formulated to maintain a constant pH of 7.4 ± 0.1 while remaining non-toxic to cells. The 21-040-CV calcium and magnesium-free formulation is the preferred PBS format for cell washing before trypsinization, EDTA-based cell detachment, and any application where divalent cations must be excluded.
The distinction between PBS (21-040-CV) and Dulbecco's Phosphate-Buffered Saline (DPBS, 21-031-CV) is important for researchers selecting the appropriate buffer for their application. While both are isotonic phosphate-buffered salines at physiological pH, DPBS was specifically developed by Renato Dulbecco for mammalian cell culture applications and contains a slightly more complex salt formulation. Standard PBS (21-040-CV) uses a simpler sodium chloride/sodium phosphate buffer system and is the more broadly applicable format across a wider range of biological, biochemical, and molecular biology applications — making it the go-to general-purpose phosphate buffer for labs that need a versatile, economical, and reliable isotonic saline buffer.
Every production lot of Mediatech PBS is low endotoxin (<0.25 EU/mL), mycoplasma tested, formulated with cell culture grade WFI (Water for Injection), and manufactured in a cGMP environment under ISO 13485 certified quality management system. The 24-month shelf life and wide storage temperature range (2°C to 30°C) provide flexibility in storage and handling. LIGHT LABS is your trusted source for Mediatech cell culture media and reagents.
KEY FEATURES
- PBS (Phosphate-Buffered Saline) 1X — simpler sodium chloride/sodium phosphate buffer system; broadly applicable across cell biology, molecular biology, and biochemistry workflows
- Without calcium and magnesium — ideal for cell washing before trypsinization and EDTA-based cell detachment; divalent cations inhibit trypsin and must be excluded
- pH 7.4 ± 0.1 — precise physiological pH maintained by phosphate buffer system; stable without CO₂ supplementation
- Isotonic formulation — maintains cell tonicity and viability during washing, transport, and dilution
- Low endotoxin: <0.25 EU/mL — suitable for sensitive mammalian cell culture and in vitro applications
- Mycoplasma tested — every production lot quality controlled
- Formulated with cell culture grade WFI (Water for Injection) — highest purity water
- Manufactured in cGMP environment under ISO 13485 certified quality management system
- 24-month shelf life — excellent stability for routine laboratory use
- Wide storage temperature range: 2°C to 30°C — refrigerator or ambient temperature storage
- Complete Certificate of Analysis available for each production lot
- Liquid, sterile, ready-to-use format; clear solution
- 500 mL bottles; 6 bottles per case
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 21-040-CV |
| Brand | Mediatech |
| Product Name | Phosphate-Buffered Saline (PBS), 1X |
| Formulation | 1X PBS, without calcium, without magnesium |
| Calcium | No — NOT included |
| Magnesium | No — NOT included |
| Concentration | 1X (ready-to-use) |
| pH | 7.4 ± 0.1 |
| Color | Clear |
| Format | Liquid, sterile, ready-to-use |
| Endotoxin Level | <0.25 EU/mL |
| Mycoplasma Testing | Yes — each lot tested |
| Water Quality | Cell culture grade WFI (Water for Injection) |
| Quality System | ISO 13485 certified; cGMP manufactured |
| Certificate of Analysis | Yes — lot-specific CoA available |
| Shelf Life | 24 months |
| Volume | 500 mL per bottle |
| Packaging | 6 bottles per case |
| Storage Temperature | 2°C to 30°C (refrigerator or ambient) |
PBS (21-040-CV) vs. DPBS (21-031-CV) — KEY DIFFERENCES
| Feature | PBS (21-040-CV) | DPBS (21-031-CV) |
|---|---|---|
| Buffer Type | Standard PBS — NaCl/NaH₂PO₄ | Dulbecco's PBS — more complex salt formulation |
| pH | 7.4 ± 0.1 | ~7.4 |
| Ca²⁺/Mg²⁺ | No | No (this formulation) |
| Shelf Life | 24 months | 36 months |
| Best For | General purpose; broad applications | Mammalian cell culture; developed specifically for cell work |
| Storage | 2°C to 30°C | 2°C to 30°C |
APPLICATIONS
- Cell washing before trypsinization — removes Ca²⁺ and Mg²⁺ that inhibit trypsin activity
- EDTA-based cell dissociation requiring calcium and magnesium-free buffer
- General cell washing between media changes and reagent treatments
- Cell transport and short-term viability maintenance outside the incubator
- Sample dilution for cell counting, flow cytometry, and ELISA
- Reagent, antibody, and biological material dilution requiring isotonic physiological pH buffer
- Western blotting and immunofluorescence washing steps
- DNA, RNA, and protein sample dilution and preparation
- General-purpose molecular biology buffer for PCR, hybridization, and other applications
- Any laboratory application requiring a sterile, isotonic, calcium and magnesium-free phosphate buffer at pH 7.4
ORDERING INFORMATION — MEDIATECH PHOSPHATE-BUFFERED SALINE (PBS)
| Cat # | Description | Ca²⁺/Mg²⁺ | Volume | Pkg |
|---|---|---|---|---|
| 21-040-CV | PBS, 1X, Without Calcium & Magnesium, pH 7.4 | No | 500 mL | 6/case |
| 21-040-CM | PBS, 1X, Without Calcium & Magnesium, pH 7.4 | No | 1 L | 6/case |
| 21-031-CV | DPBS (Dulbecco's), 1X, Without Calcium & Magnesium | No | 500 mL | 6/case |
| 20-030-CV | DPBS (Dulbecco's), 1X, With Calcium & Magnesium | Yes | 500 mL | 6/case |
| 46-013-CV | PBS, 1X, Without Calcium & Magnesium, Without Phenol Red | No | 500 mL | 6/case |
| 55-040-CV | PBS, 10X, Without Calcium & Magnesium (concentrate) | No | 500 mL | 6/case |
