Product Description
LIGHT LABS is proud to offer Mediatech RPMI 1640 with 25 mM HEPES and Without L-Glutamine (Cat # 15-041-CV) — the most flexible and pH-stable formulation in the Mediatech RPMI 1640 lineup, combining 25 mM HEPES buffer for superior pH stability outside the CO₂ incubator with a glutamine-free formulation for complete independent control over nitrogen source supplementation. The 15-041-CV is the preferred choice for researchers requiring both HEPES buffering AND glutamine flexibility in RPMI 1640 — and features lower documented endotoxin content than offerings from other major suppliers, a critical quality advantage for the sensitive lymphoid, hybridoma, and hematopoietic cell types for which RPMI 1640 is specifically optimized.
The 25 mM HEPES buffer in the 15-041-CV provides robust pH stability across a wider range of CO₂ concentrations and atmospheric conditions than bicarbonate buffering alone — making this formulation the preferred choice for high-throughput cell culture workflows where plates and flasks spend extended time outside the CO₂ incubator during liquid handling, for core facility environments where incubator CO₂ levels may fluctuate, and for any application where stable pH during bench manipulations is critical. Unlike the bicarbonate-only buffered 15-040-CV (RPMI without L-glutamine) and 10-040-CV (RPMI with L-glutamine) where pH can shift rapidly during bench manipulations, the dual bicarbonate + 25 mM HEPES buffer system of the 15-041-CV maintains physiological pH robustly under all standard cell culture conditions.
The absence of L-glutamine makes the 15-041-CV ideal for researchers using GlutaMAX™ or Corning® GlutaGRO™ stable dipeptide glutamine substitutes, studying glutamine metabolism in lymphoid cells, or requiring precise independent control over glutamine concentration in their RPMI 1640 formulation. The combination of HEPES buffering and glutamine-free flexibility in a single formulation eliminates the need to stock two separate RPMI variants for labs requiring both features. Every production lot undergoes comprehensive quality testing with a lot-specific Certificate of Analysis. Manufactured under ISO 13485 certified quality management system. LIGHT LABS is your trusted source for Mediatech cell culture media and reagents.
KEY FEATURES
- RPMI 1640 with 25 mM HEPES AND without L-glutamine — the most flexible formulation; combines superior pH stability with maximum glutamine supplementation control in a single bottle
- Lower documented endotoxin than offerings from other major suppliers — critical quality advantage for sensitive lymphoid, hybridoma, and hematopoietic cell types
- 25 mM HEPES buffer — robust pH stability outside the CO₂ incubator; ideal for high-throughput workflows, core facilities, and extended bench manipulations
- Without L-glutamine — maximum flexibility; add L-glutamine, GlutaMAX™, or Corning® GlutaGRO™ at precisely defined concentrations before use
- Eliminates need to stock two separate RPMI variants — one formulation provides both HEPES buffering AND glutamine flexibility
- Ideal for GlutaMAX™ or Corning® GlutaGRO™ users — add stable dipeptide glutamine substitute to glutamine-free HEPES-buffered RPMI
- Critical for glutamine metabolism studies in lymphoid cells — precise control of both pH and glutamine availability in the same formulation
- Cell types supported: mouse hybridomas, human leukocytes, B and T lymphocytes, myeloma cells, PBMCs, leukemic cell lines (add glutamine before use)
- Comprehensive lot-specific quality control — growth promotion, cytotoxicity, endotoxin, sterility, and mycoplasma testing on every lot
- Complete Certificate of Analysis available for each production lot
- Manufactured under ISO 13485 certified quality management system
- Liquid, sterile, ready-to-use format (add glutamine source before use)
- Storage: 2°C to 8°C
- 500 mL bottles; 6 bottles per case
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 15-041-CV |
| Brand | Mediatech |
| Product Name | RPMI 1640, With 25 mM HEPES, Without L-Glutamine |
| L-Glutamine | No — NOT included; add before use |
| HEPES | Yes — 25 mM |
| Sodium Bicarbonate | Yes — included |
| Phenol Red | Yes — included |
| Endotoxin | Lower than major competing suppliers |
| Format | Liquid, sterile, ready-to-use (add glutamine before use) |
| Sterility Testing | Yes — each lot tested |
| Mycoplasma Testing | Yes — each lot tested |
| Quality System | ISO 13485 certified |
| Certificate of Analysis | Yes — lot-specific CoA available |
| Volume | 500 mL per bottle |
| Packaging | 6 bottles per case |
| Storage Temperature | 2°C to 8°C |
MEDIATECH RPMI 1640 LINEUP — CHOOSING THE RIGHT FORMULATION
| Cat # | L-Glutamine | HEPES | Best For |
|---|---|---|---|
| 15-041-CV | No | 25 mM | Maximum flexibility + pH stability; GlutaMAX™; glutamine studies |
| 10-041-CV | Yes | 25 mM | HEPES buffering with glutamine included |
| 15-040-CV | No | No | Glutamine-free; standard CO₂ incubator |
| 10-040-CV | Yes | No | Routine lymphocyte/hybridoma culture; standard CO₂ incubator |
APPLICATIONS
- Cell culture requiring both HEPES pH stability and independent glutamine control in RPMI 1640
- Hybridoma and myeloma cell culture with GlutaMAX™ or Corning® GlutaGRO™ in HEPES-buffered RPMI
- High-throughput lymphoid cell culture workflows requiring stable pH during extended bench manipulations
- Core facility RPMI 1640 for environments with variable incubator CO₂ levels
- Glutamine metabolism studies in lymphoid cells requiring HEPES-buffered glutamine-free RPMI
- B and T lymphocyte culture and activation protocols requiring pH stability
- Any RPMI 1640 application requiring dual HEPES buffering AND glutamine-free flexibility
ORDERING INFORMATION — MEDIATECH RPMI 1640 FORMULATIONS
| Cat # | Description | L-Gln | HEPES | Volume | Pkg |
|---|---|---|---|---|---|
| 15-041-CV | RPMI 1640, 25 mM HEPES, Without L-Glutamine | No | 25 mM | 500 mL | 6/case |
| 10-041-CV | RPMI 1640, 25 mM HEPES, With L-Glutamine | Yes | 25 mM | 500 mL | 6/case |
| 15-040-CV | RPMI 1640, Without L-Glutamine | No | No | 500 mL | 6/case |
| 10-040-CV | RPMI 1640, With L-Glutamine | Yes | No | 500 mL | 6/case |
| 10-040-CM | RPMI 1640, With L-Glutamine | Yes | No | 1 L | 6/case |
| 10-016-CV | IMDM, L-Glutamine, 25 mM HEPES | Yes | 25 mM | 500 mL | 6/case |
| 10-013-CV | DMEM, High Glucose, L-Glutamine, Sodium Pyruvate | Yes | No | 500 mL | 6/case |
| 15-017-CV | DMEM, High Glucose, No L-Glutamine, No Sodium Pyruvate | No | No | 500 mL | 6/case |
