Product Description
The MTC Bio OptiWell™ D3096-15 is a 96-well deep well plate with round U-bottom wells and 1.0mL capacity per well — non-sterile, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Case of 50 plates — 10 bags of 5 plates each.
Round U-Bottom Wells — Optimized for Bacterial Culture and Sample Storage The U-bottom (round bottom) well geometry of the D3096-15 is the preferred format for bacterial culture, cell suspension culture, mixing applications, and general sample storage — where the rounded well base promotes efficient resuspension of pelleted cells or beads, supports uniform mixing during shaking incubation, and eliminates the sharp corners that can retain residual liquid in V-bottom well formats. The round well cross-section provides maximum compatibility with the widest range of pipette tips, robotic liquid handling probes, and multichannel pipettes — making the D3096-15 the most versatile format in the 1.0mL 96-well deep well range for mixed-use lab environments where the same plate format must accommodate multiple workflow types.
1.0mL Per Well — The Standard Volume for 96-Well Deep Well Bacterial Culture The 1.0mL per-well capacity of the D3096-15 is the most widely used volume for 96-well deep well bacterial culture workflows — providing sufficient volume for overnight liquid culture of E. coli and other bacterial strains in 96-well format with adequate headspace for aeration during shaking incubation. The 1.0mL format also provides an important practical advantage over the larger 2.0mL format for compound management and sample storage applications — the smaller well depth reduces the volume of sample lost to dead volume during automated liquid handling aspiration while still providing ample working volume for most standard 96-well deep well protocols.
D3096-15 vs. the OptiWell™ 96-Well Deep Well Family — Key Comparisons:
| Cat. # | Wells | Bottom | Volume | Sterile | Case Qty |
|---|---|---|---|---|---|
| D3096-07 | 96 Round | V-bottom | 0.45mL | No | 100 |
| D3096-09 | 96 Square | V-bottom | 0.5mL | No | 50 |
| D3096-15 | 96 Round | U-bottom | 1.0mL | No | 50 |
| D3096-17 | 96 Round | V-bottom | 1.0mL | No | 50 |
| D3096-27 | 96 Round | U-bottom | 1.2mL | No | 50 |
| D3096-33 | 96 Square | V-bottom | 1.6mL | No | 50 |
| D3096-35 | 96 Round | U-bottom | 2.0mL | No | 50 |
D3096-15 vs. D3096-17 — U-Bottom vs. V-Bottom at 1.0mL: Both the D3096-15 and D3096-17 are 96-well deep well plates with 1.0mL per-well capacity and round wells — the well bottom geometry is the only difference:
| D3096-15 | D3096-17 | |
|---|---|---|
| Well Bottom | U-bottom (round) | V-bottom (conical) |
| Best For | Bacterial culture, mixing, cell suspension | Sample recovery, compound management, bead assays |
| Resuspension | Excellent — rounded base promotes uniform mixing | Good — conical base concentrates pellet |
| Sample Recovery | Good | Maximum — V concentrates to lowest point |
| Volume | 1.0mL | 1.0mL |
Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-15 conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, and other standard HTS robotic systems. The standard footprint, well spacing, and plate height ensure reliable robotic tip alignment and sample transfer across all 96 wells.
4,000 × g Centrifugation Rating — Full Plate Centrifuge Compatibility Centrifugation up to 4,000 × g. The robust polypropylene construction of the D3096-15 withstands centrifugal forces up to 4,000 × g — covering the full range of standard plate centrifugation protocols used in bacterial cell pelleting, bead collection, sample concentration, and any workflow requiring centrifugation of 96-well deep well plate contents.
Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Manufactured from virgin (non-recycled) medical-grade polypropylene — free from recycled resin contaminants, processing aids, and extractables. The medical-grade formulation provides minimal non-specific binding of biological molecules and minimal liquid retention on the well surface after aspiration.
Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns) allows instant identification of specific wells during liquid handling setup, plate reading, and data recording.
Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. All plates are certified non-pyrogenic and RNase/DNase free.
Compatible with Silicone Sealing Mats and Sealing Film The D3096-15 can be used with silicone sealing mats and various types of sealing film — allowing labs to select the sealing format most appropriate for their specific application, incubation conditions, and storage requirements.
Compatible Replacement — Cross-Reference Guide
The MTC Bio OptiWell™ D3096-15 is a direct compatible replacement for 96-well round U-bottom 1.0mL deep well plates from several major manufacturers:
- Thermo Fisher AB-0661 — Thermo Scientific 96-well round U-bottom deep well plate, 1.0mL, non-sterile
- Greiner Bio-One 780271 — 96-well deep well plate, U-bottom, 1.0mL, polypropylene
- Eppendorf® 0030501306 — Eppendorf 96-well deepwell plate, U-bottom, 1.0mL
Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.
Key Features
- MTC Bio OptiWell™ — Catalog # D3096-15
- 96-well deep well plate — round U-bottom wells
- 1.0mL per-well capacity — standard deep well bacterial culture volume
- Round U-bottom — promotes uniform mixing and resuspension for bacterial culture and cell suspension
- Virgin medical-grade polypropylene — minimal binding and liquid retention
- Black printed alpha-numerics — instant well identification
- ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
- 4,000 × g centrifugation rating
- Compatible with silicone sealing mats and sealing film
- No lid, no skirt
- Non-sterile (autoclavable)
- Class 100,000 cleanroom production
- Certified RNase-free and DNase-free
- Certified non-pyrogenic
- Sigma-Aldrich catalog # MTCD309615
- Case of 50 plates — 10 bags of 5 plates
Supported Applications 96-well format bacterial liquid culture (overnight and expression cultures), cell suspension culture in 96-well deep well format, compound dilution and preparation at 1.0mL scale, automated liquid handling sample reformatting, bead-based assay preparation, centrifugation workflows up to 4,000 × g, sample storage and archiving, DNA/RNA extraction in 96-well format using magnetic bead-based systems, protein expression in 96-well bacterial culture format, serial dilution series at 1.0mL scale, and any 96-well application requiring round U-bottom deep well format with 1.0mL per-well capacity and full automated platform compatibility.
Technical Specifications
| Specification | Value |
|---|---|
| SKU / Catalog # | D3096-15 |
| Sigma-Aldrich Catalog # | MTCD309615 |
| Manufacturer | MTC Bio |
| Product Line | OptiWell™ |
| Well Format | 96-well |
| Well Shape | Round |
| Well Bottom | U-bottom (round) |
| Well Volume / Capacity | 1.0mL per well |
| Material | Virgin medical-grade polypropylene |
| Color | Clear |
| Alpha-Numerics | Yes — black printed |
| Lid | No |
| Skirt | No |
| Centrifugation Rating | 4,000 × g |
| Binding | Minimal — low binding and liquid retention |
| Sealing Compatibility | Silicone sealing mats and sealing film |
| Sterility | Non-sterile |
| Autoclavable | Yes |
| Footprint | ANSI/SLAS 1-2004 compliant |
| Production Environment | Class 100,000 cleanroom |
| RNase-Free | Yes — certified |
| DNase-Free | Yes — certified |
| Non-Pyrogenic | Yes — certified |
| Case Quantity | 50 plates (10 bags × 5 plates) |