MTC Bio OptiWell™ D3096-43S | 96-Well Deep Well Plate, Square U-Bottom (H-Style), 2.2mL, Sterile — 50/Case (10 Bags of 5)

The MTC Bio OptiWell™ D3096-43S is a 96-well deep well plate with square U-bottom (H-style) wells and 2.2mL capacity per well — sterile by gamma irradiation, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment including KingFisher™ and Accuris IsoPure™ magnetic bead platforms. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Sterilized by gamma irradiation. Case of 50 plates — 10 bags of 5 plates each.

Square U-Bottom H-Style Wells — The Standard Format for Magnetic Bead Automation The square U-bottom H-style well geometry of the D3096-43S is the defining format for 96-well deep well plates used in magnetic bead-based nucleic acid extraction workflows on automated platforms. The square well cross-section maximizes the volume capacity of each well within the standard 96-well footprint while the U-bottom (round base) promotes uniform resuspension and mixing of magnetic beads during agitation steps. The "H-style" designation refers to the specific square-well-with-rounded-base geometry widely recognized as the standard deep well plate format for KingFisher™ Flex and Duo Prime magnetic particle processors, Accuris IsoPure™ magnetic bead systems, and other automated magnetic bead DNA/RNA extraction platforms. For labs running automated nucleic acid extraction workflows, the D3096-43S H-style format is the required plate geometry for compatibility with magnetic tip comb systems used by these instruments.

2.2mL Per Well — Maximum Volume for 96-Well Deep Well Applications The 2.2mL per-well capacity of the D3096-43S is the largest standard volume available in the 96-well deep well format — providing the maximum working volume for bacterial culture, large-volume sample processing, and automated magnetic bead extraction workflows requiring sufficient buffer volumes across wash and elution steps. For automated nucleic acid extraction protocols on KingFisher™ and equivalent platforms, the 2.2mL capacity accommodates the full volume of lysis buffer, wash buffers, and elution buffer required for complete nucleic acid extraction workflows without the risk of well overflow during bead mixing steps.

Sterile by Gamma Irradiation — Validated for Cell Culture and Sterile Workflows Sterile. Sterilized by gamma irradiation. The D3096-43S is sterilized by gamma irradiation — providing validated, reliable sterility without heat damage or residual chemical concerns. The "S" suffix in the catalog number designates the sterile format — distinguishing it from the non-sterile D3096-43 version. Gamma irradiation sterilization qualifies the D3096-43S for bacterial culture, cell-based assays, sterile nucleic acid extraction workflows, and any application requiring validated sterile plasticware. Sterile according to ISO 13485.

D3096-43S vs. D3096-43 — Sterile vs. Non-Sterile: The Only Difference The D3096-43S and D3096-43 are identical OptiWell™ 96-well square U-bottom H-style 2.2mL deep well plates — sterility is the sole difference:

D3096-43S vs. the OptiWell™ 96-Well Deep Well Family — Key Comparisons:

D3096-43S vs. D3096-41S — H-Style vs. Standard at 2.2mL Sterile: Both are sterile 96-well square U-bottom 2.2mL deep well plates — the well geometry designation differs:

Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-43S conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, KingFisher™, Accuris IsoPure™, and other standard HTS robotic systems.

4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g. The robust polypropylene construction of the D3096-43S withstands centrifugal forces up to 4,000 × g — covering all standard plate centrifugation protocols used in bead collection, cell pelleting, sample concentration, and nucleic acid extraction workflows.

Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Manufactured from virgin medical-grade polypropylene — free from recycled resin contaminants, processing aids, and extractables. Certified RNase/DNase free and non-pyrogenic.

Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification allows instant identification of specific wells during liquid handling setup, plate reading, and data recording.

Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. Certified non-pyrogenic and RNase/DNase free.

Compatible Replacement — Cross-Reference Guide

The MTC Bio OptiWell™ D3096-43S is a direct compatible replacement for 96-well square U-bottom 2.2mL sterile deep well plates from several major manufacturers:

• Thermo Fisher 95040460 — KingFisher™ 96 deep well plate, square U-bottom, 2.2mL, sterile
• Greiner Bio-One 780270 — 96-well deep well plate, square U-bottom, 2.2mL, sterile
• Eppendorf® 0030501328 — Eppendorf 96-well deepwell plate, square U-bottom, 2.2mL, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Key Features

• MTC Bio OptiWell™ — Catalog # D3096-43S
• 96-well deep well plate — square U-bottom H-style wells
• 2.2mL per-well capacity — maximum volume in 96-well deep well format
• Square U-bottom H-style — compatible with KingFisher™ and Accuris IsoPure™ magnetic bead platforms
• Sterile — gamma irradiation, ISO 13485
• Virgin medical-grade polypropylene — minimal binding and liquid retention
• Black printed alpha-numerics — instant well identification
• ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
• 4,000 × g centrifugation rating
• Compatible with silicone sealing mats and sealing film
• No lid, no skirt
• Class 100,000 cleanroom production
• Certified RNase-free and DNase-free
• Certified non-pyrogenic
• Sigma-Aldrich catalog # MTCD309643S
• Case of 50 plates — 10 bags of 5 plates

Supported Applications KingFisher™ Flex and Duo Prime magnetic bead nucleic acid extraction workflows, Accuris IsoPure™ magnetic bead extraction, automated 96-well magnetic bead DNA/RNA extraction, bacterial liquid culture in 96-well sterile format, mammalian cell culture in deep well format, compound dilution and preparation at 2.2mL scale, automated liquid handling sample reformatting requiring sterile plates, bead-based immunoprecipitation, sterile sample storage, and any 96-well application requiring square U-bottom H-style deep well format at 2.2mL capacity with validated sterility and full automated platform compatibility.

Technical Specifications