Product Description
Capture nearly every RNA biotype, not just polyadenylated transcripts. This Zymo Research kit produces NGS-grade first-strand cDNA with ribosomal RNA removed, giving you a versatile starting point for qPCR, RNA-seq library construction and other analyses from any organism. Because rRNA is removed during cDNA synthesis, there is no separate depletion step to manage.
This listing is for catalog number R3001: Zymo-Seq RiboFree Universal cDNA Kit, 12 Preps. RiboFree Universal Depletion removes rRNA without probes and has been validated on mammalian, plant and prokaryotic RNA from cells, snap-frozen tissue and FFPE samples. Unlike poly(A) selection, it retains full-length mRNA, mRNA with degraded tails, lncRNA, intronic and nucleolar RNA. All required reagents, including SPRI beads, are supplied for 12 preparations. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Universal rRNA depletion – probe-free removal of ribosomal RNA from any organism.
- Broad biotype coverage – retains mRNA, degraded-tail mRNA, lncRNA, intronic and nucleolar RNA.
- NGS-grade output – single-stranded cDNA ready for library prep or qPCR.
- FFPE compatible – validated with fresh, snap-frozen and FFPE samples.
- Complete kit – includes all reagents, SPRI beads among them.
Specifications
| Catalog Number | R3001 |
| Brand | Zymo Research |
| Description | Zymo-Seq RiboFree Universal cDNA Kit, 12 Preps |
| Output | rRNA-depleted single-stranded cDNA |
| rRNA depletion | RiboFree Universal Depletion (probe-free) |
| Validated samples | Cells, snap-frozen tissue, FFPE |
| Validated organisms | Mammals, plants, various prokaryotes |
| Included | All reagents including SPRI beads |
| Kit size | 12 preps |
| Shipping Conditions | Ships on dry ice |
| Storage | -80C, -20C, 4C |
| Note | Product contains multiple components shipped separately |
Applications
- Custom RNA-seq library construction
- qPCR detection from rRNA-depleted cDNA
- Degraded and FFPE RNA studies
- Noncoding RNA research
