Zymo Research DNA Clean & Concentrator™-100 D4030 | 50 Preps with Zymo-Spin™ V Column with Reservoir

The Zymo Research DNA Clean & Concentrator™-100 D4030 is the 50-preparation large-capacity DNA cleanup and concentration kit — the high-volume format in the DCC-100 line, designed for rapid desalting and purification of up to 100µg of high-quality DNA from PCR, large format restriction endonuclease digestions, or cell-free lysates. Supplied with the unique Zymo-Spin™ V Column with Reservoir — a large-format spin column with an integrated reservoir accommodating sample volumes up to 15mL for loading without requiring multiple reload steps. Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, and cell-free DNA preps. Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, vacuum source, or syringe. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, and radiolabeling. 50 preparations. Catalog # D4030. The 25-preparation version is available as D4029.

D4030 vs. D4029 — Choosing the Right Kit Size: The D4030 and D4029 are identical DNA Clean & Concentrator™-100 kits in every way — the number of preparations and reagent volumes are the sole differences:

The D4030 is the preferred choice for labs and core facilities that routinely perform large-scale DNA purification — eliminating the frequent reordering cycles required with the 25-prep D4029 and providing a lower per-prep reagent cost for established, high-volume workflows.

The DCC-100 — Built for Large-Scale DNA Cleanup The DNA Clean & Concentrator™-100 occupies a critical and distinct position in the Zymo Research DCC family — designed specifically for large-scale DNA purification where the standard DCC-5 (up to 5µg) or DCC-25 (up to 25µg) formats are insufficient:

Zymo-Spin™ V Column with Reservoir — 15mL Large-Volume Loading The Zymo-Spin™ V Column with Reservoir accommodates sample volumes up to 15mL per load without requiring multiple centrifugation cycles — dramatically simplifying the loading step for large-volume samples such as large-scale PCR reaction pools, large restriction digest volumes, and cell-free lysate preparations. The column is also compatible with vacuum manifolds and syringe-based loading — providing flexibility for labs with different equipment setups.

Up to 100µg DNA Per Prep — Large-Scale Purification at High Volume Up to 100µg total DNA can be eluted with ≥150µL of low-salt DNA Elution Buffer or water — 20× the capacity of the standard DCC-5. The D4030 delivers this large-capacity purification consistently across all 50 preparations — making it the highest-throughput format in the DCC-100 line for labs running large-scale DNA cleanup routinely.

DNA Size Range — 50bp to 23kb DNA Size Limits — from ~50bp to 23kb. The DCC-100 recovers DNA fragments across the full range of standard molecular biology applications — from short PCR amplicons through large restriction digest fragments, plasmid linearization products, and large DNA constructs used in cloning and genomics workflows.

High Purity DNA — A260/A280 ≥1.8 DNA Purity — High-quality DNA (A260/A280 ≥1.8) ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures. The consistently high purity is maintained across all 50 preparations of the D4030 — ensuring reliable downstream application performance throughout the full kit lifetime.

Multiple Loading Methods — Centrifuge, Vacuum, or Syringe Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, vacuum source, or syringe. The flexible loading options of the DCC-100 are preserved across the full 50-preparation D4030 kit — compatible with essentially any laboratory equipment configuration.

Less Than 20 Minutes — Complete Purification at Scale The entire DNA purification/concentration procedure typically takes less than 20 minutes — maintained consistently across all 50 preparations of the D4030. For high-volume labs processing multiple large-format samples per day, the consistent sub-20-minute protocol allows accurate throughput scheduling.

Room Temperature Storage — All Kit Components All kit components stored at room temperature — simplifying storage of the larger D4030 reagent volumes compared to the D4029. Note: Ethanol must be added to DNA Wash Buffer concentrate prior to use as indicated on the label.

Complete Kit Contents — D4030 (50 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research DNA Clean & Concentrator™-100 D4030 is a comparable alternative to large-capacity DNA cleanup kits from several major manufacturers:

• Qiagen QIAquick PCR Purification Kit (28106) — up to 10µg DNA cleanup (smaller capacity)
• Thermo Fisher GeneJET PCR Purification Kit (K0702) — up to 25µg DNA cleanup
• Monarch PCR & DNA Cleanup Kit NEB (T1030L) — up to 50µg DNA cleanup
• Macherey-Nagel NucleoSpin Gel and PCR Clean-up (740609) — up to 25µg DNA cleanup

The D4030 uniquely provides up to 100µg DNA purification capacity across 50 preparations — larger than most competing standard PCR cleanup kits. These products may differ in protocol, yield, and performance characteristics.

Key Features

• Zymo Research DNA Clean & Concentrator™-100 — Catalog # D4030
• 50 preparations with Zymo-Spin™ V Column with Reservoir — high-volume format
• Up to 100µg total DNA per prep — large-scale purification capacity
• 150µL minimum elution volume — highly concentrated large-mass DNA
• DNA size range: ~50bp to 23kb
• A260/A280 ≥1.8 — high-purity DNA ready for all downstream applications
• Zymo-Spin™ V Column with Reservoir — up to 15mL sample loading per cycle
• Multiple loading methods: centrifuge, microcentrifuge, vacuum, or syringe
• Complete purification in less than 20 minutes
• Ethanol required — must be added to DNA Wash Buffer concentrate before use
• Room temperature storage — all kit components
• 1-year kit component integrity guarantee
• Lot-to-lot tested for performance and reliability
• Lowest per-prep cost in the DCC-100 line
• Smaller pack size: D4029 (25 preps)

Supported Applications Large-scale PCR reaction pool cleanup and desalting, large-format restriction endonuclease digest purification, cell-free lysate DNA purification, scale-up PCR product cleanup for preparative molecular cloning, large-scale plasmid linearization cleanup, DNA purification for transfection and transformation at scale, NGS library cleanup from large-volume reactions, preparative gel excision cleanup, radioactive PCR reaction cleanup and desalting, RNA transcription template preparation, and any high-throughput molecular biology workflow requiring purification of up to 100µg of DNA from large-volume reactions in less than 20 minutes.

Technical Specifications