Zymo Research DNA Clean & Concentrator™-5 D4003 | 50 Preps with Zymo-Spin™ I Columns (Uncapped)

The Zymo Research DNA Clean & Concentrator™-5 D4003 is the 50-preparation PCR purification and DNA cleanup kit with uncapped Zymo-Spin™ I Columns — providing purification of up to 5µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitates the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc. Clean and concentrate up to 5µg DNA with ≥6µl elution in as little as 2 minutes with 0µl wash residue carryover. Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer. Supplied with uncapped Zymo-Spin™ I Columns. 50 preparations. Catalog # D4003. The capped column version is D4013. The 200-prep uncapped version is D4004. 

D4003 vs. D4013 — Uncapped vs. Capped Columns: The Only Difference The D4003 and D4013 are identical DNA Clean & Concentrator™-5 kits in every way — the column cap is the sole difference:

The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing. 

2-Minute DNA Cleanup — The Fastest Spin Column Kit Available Clean and concentrate up to 5µg DNA with ≥6µl elution in as little as 2 minutes with 0µl wash residue carryover. The DCC-5 is the fastest spin column DNA cleanup kit available — completing the full bind-wash-elute protocol in just 2 minutes per sample. For labs processing dozens of PCR reactions per day, the 2-minute protocol dramatically reduces total processing time compared to standard PCR cleanup kits that require 15+ minutes per prep. 

Complete DCC-5 Family — Understanding All Catalog Numbers:

Up to 5µg DNA — 70–95% Recovery from 50bp to 10kb ≤5µg total DNA can be recovered. For DNA 50bp to 10kb the recovery is 70–95%. For DNA 11kb to 23kb the recovery is 50–70%. The recovery rate of the DCC-5 varies by fragment size — providing excellent recovery across the range of standard PCR amplicons, restriction digest fragments, and cloning inserts, with good recovery extending into the larger genomic fragment range up to 23kb. 

≥6µL Minimum Elution — Maximum DNA Concentration Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer. The ≥6µL minimum elution volume of the DCC-5 produces the most highly concentrated DNA eluate available from a standard PCR cleanup kit — up to 4× more concentrated than standard 25µL elution cleanup kits. For downstream applications requiring maximum DNA concentration — including NGS library preparation, Sanger sequencing, transfection, and single-cell workflows — the DCC-5 concentrated eluate delivers better results than dilute elutions from competing kits.

Zero Buffer Retention — No Wash Residue Carryover Zymo-Spin™ columns are designed for complete elution with no buffer retention or carryover. The unique Zymo-Spin™ I column geometry is engineered for zero buffer retention — eliminating residual wash buffer in the column after centrifugation without requiring a dry spin step. Wash buffer carryover into the eluate is a significant source of downstream enzymatic inhibition in standard PCR cleanup kits — the DCC-5 eliminates this entirely, producing a clean eluate that performs reliably in ligation, sequencing, transfection, and restriction digest applications. 

Broad Sample Compatibility — Removes All Major PCR Inhibitors The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitates the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Compatible sample sources include: 

• PCR reactions — standard, long-range, high-fidelity, hot-start
• Restriction endonuclease digests — single and double digest cleanup
• DNA modification reactions — end repair, A-tailing, ligation, phosphorylation/dephosphorylation
• Isotope and fluorescence labeling reactions — removal of unincorporated labels
• NGS library preparation — adapter ligation cleanup, end repair cleanup, size selection cleanup
• Bisulfite conversion — pre- and post-conversion DNA cleanup
• ChIP DNA — chromatin immunoprecipitation sample cleanup (see also D5201)
• Sequencing reactions — post-cycle sequencing dye terminator cleanup

High Purity DNA — A260/A280 ≥1.8 DNA Purity — High-quality DNA (A260/A280 ≥1.8) ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures. The consistently high A260/A280 purity of DCC-5 eluates meets or exceeds the purity standards required for all downstream molecular biology applications — including NGS library preparation, Sanger sequencing, and transfection where DNA purity directly determines results quality. 

Extensively Cited in Published Research — One of the Most Referenced DNA Cleanup Kits The Zymo Research DNA Clean & Concentrator™-5 is one of the most widely cited DNA cleanup kits in published molecular biology research — referenced in thousands of peer-reviewed publications across genomics, epigenomics, NGS, CRISPR, and molecular cloning. When a published protocol specifies D4003, DCC-5, or DNA Clean & Concentrator-5, this is the correct uncapped column product.

Room Temperature Storage — All Kit Components Storage Temperature — Room Temp for all kit components. Note: Ethanol must be added to DNA Wash Buffer concentrate prior to use as indicated on the label. 

Complete Kit Contents — D4003 (50 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research DNA Clean & Concentrator™-5 D4003 is a direct comparable alternative to PCR purification kits from several major manufacturers:

• Qiagen QIAquick PCR Purification Kit (28104) — 50-prep PCR cleanup kit, 30µL minimum elution
• Thermo Fisher GeneJET PCR Purification Kit (K0701) — 50-prep PCR cleanup kit
• NEB Monarch PCR & DNA Cleanup Kit (T1030S) — 50-prep PCR cleanup kit
• Macherey-Nagel NucleoSpin Gel and PCR Clean-up (740609) — PCR cleanup kit

The D4003 differentiates with ≥6µL minimum elution (vs. 30µL for QIAquick), 2-minute protocol, and zero buffer retention. These products may differ in protocol, yield, and performance characteristics.

Key Features

• Zymo Research DNA Clean & Concentrator™-5 — Catalog # D4003
• 50 preparations with Zymo-Spin™ I Columns (Uncapped)
• Up to 5µg total DNA per prep
• ≥6µL minimum elution volume — maximum DNA concentration
• 2-minute cleanup procedure — fastest spin column DNA cleanup available
• Zero buffer retention — no wash residue carryover, no dry spin required
• DNA recovery: 70–95% for 50bp–10kb; 50–70% for 11kb–23kb
• DNA size range: ~50bp to 23kb
• A260/A280 ≥1.8 — high-purity DNA ready for all downstream applications
• Removes DNA polymerases, restriction enzymes, ligases, kinases, nucleases, phosphatases, dNTPs, and labeled nucleotide analogs
• Compatible with PCR reactions, restriction digests, modification reactions, labeling reactions, NGS library prep steps
• Uncapped columns — faster processing (capped version: D4013)
• Room temperature storage — all kit components
• Ethanol must be added to DNA Wash Buffer concentrate before use
• 1-year kit component integrity guarantee
• Lot-to-lot tested for performance and reliability
• Capped column version: D4013 (50 preps)
• 200-prep uncapped version: D4004

Supported Applications PCR product cleanup and desalting, restriction endonuclease digest purification, DNA end repair and A-tailing cleanup, ligation reaction cleanup, DNA dephosphorylation/phosphorylation reaction cleanup, isotope and fluorescence label removal, NGS library preparation cleanup (adapter ligation, end repair, size selection), bisulfite conversion pre- and post-conversion cleanup, Sanger sequencing template preparation, DNA concentration from dilute samples, CRISPR donor DNA preparation, transfection-grade PCR product preparation, radiolabeled probe cleanup, and any molecular biology workflow requiring rapid, highly concentrated, ultra-pure DNA in 2 minutes.

Technical Specifications