Product Description
LIGHT LABS is proud to offer the Zymo Research DNA Clean & Concentrator™-500 (Cat # D4031) — the highest capacity kit in Zymo Research's industry-leading DNA Clean & Concentrator™ (DCC) product line, now in a convenient 10-prep format. Designed for the rapid, large-format purification and concentration of up to 500 µg of high-quality DNA in a single column, the DCC-500 is the go-to solution for researchers working with large-scale restriction endonuclease digestions, crude DNA preparations, and other high-input DNA workflows where standard cleanup kits fall short.
Using Zymo Research's Zymo-Spin™ VI column technology, the DCC-500 delivers ultra-pure DNA (A260/280 ≥ 1.8) in less than 20 minutes. The unique large-format column construction allows sample loading and washing to be performed using either a centrifuge, microcentrifuge, or vacuum source — providing flexibility for a wide range of lab setups. Purified DNA is ideally suited for PCR, DNA sequencing, DNA transfection, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, and microarray applications. The D4031 10-prep format is ideal for labs with occasional large-scale cleanup needs or those evaluating the DCC-500 for the first time. LIGHT LABS is your trusted source for Zymo Research products.
KEY FEATURES
- Highest capacity DNA cleanup kit in the Zymo Research DCC line — processes up to 500 µg of DNA per column
- Complete purification and concentration in less than 20 minutes
- High DNA purity — A260/280 ≥ 1.8, ideal for all downstream applications requiring ultra-clean DNA
- DNA size range: ~50 bp to 23 kb
- DNA recovery: 70–95% for 50 bp to 10 kb; 50–70% for 11 kb to 23 kb
- Unique Zymo-Spin™ VI large-format column compatible with centrifuge, microcentrifuge, or vacuum source for flexible processing
- Minimum elution volume: ≥ 2 mL
- Detergent tolerant: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
- Effectively removes primers, nucleotides, enzymes, salts, and other enzymatic reaction impurities
- Eluted DNA immediately ready for PCR, sequencing, ligation, transfection, restriction digestion, RNA transcription, radiolabeling, and microarray applications
- 10-prep format — ideal for occasional large-scale use or first-time evaluation
- Fisher Scientific Catalog No. 50-444-214
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | D4031 |
| Fisher Scientific Catalog No. | 50-444-214 |
| Brand | Zymo Research |
| Kit Name | DNA Clean & Concentrator™-500 (DCC-500) |
| Number of Preps | 10 |
| Maximum DNA Binding Capacity | 500 µg |
| DNA Size Range | ~50 bp to 23 kb |
| DNA Recovery | 70–95% (50 bp – 10 kb); 50–70% (11 kb – 23 kb) |
| DNA Purity | A260/280 ≥ 1.8 |
| Column Type | Zymo-Spin™ VI (large format) |
| Processing Time | Less than 20 minutes |
| Processing Method | Centrifuge, microcentrifuge, or vacuum |
| Minimum Elution Volume | ≥ 2 mL |
| Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| Storage Temperature | Room temperature |
| Kit Components | DNA Binding Buffer (100 mL), DNA Wash Buffer concentrate (24 mL), DNA Elution Buffer (2 x 16 mL), Zymo-Spin™ VI Columns (10), Instruction Manual |
APPLICATIONS
- Large-scale PCR product cleanup and concentration
- Large-scale restriction endonuclease digestion cleanup
- Crude DNA preparation purification
- DNA sequencing sample preparation
- DNA transfection and ligation
- RNA transcription template preparation
- Radiolabeling reaction cleanup
- Microarray and hybridization sample preparation
- Cell-free lysate DNA purification
- Any application requiring high-capacity, rapid DNA purification from large-volume or high-input samples
ORDERING INFORMATION
| Cat # | Description | Max Capacity | Preps |
|---|---|---|---|
| D4031 | DNA Clean & Concentrator™-500 | 500 µg | 10 |
| D4032 | DNA Clean & Concentrator™-500 | 500 µg | 20 |
| D4029 | DNA Clean & Concentrator™-100 | 100 µg | 10 |
| D4030 | DNA Clean & Concentrator™-100 | 100 µg | 25 |
| D4013 | DNA Clean & Concentrator™-5 | 5 µg | 25 |
| D4014 | DNA Clean & Concentrator™-5 | 5 µg | 50 |
| D4033 | DNA Clean & Concentrator™-5 | 5 µg | 25 |
| D4034 | DNA Clean & Concentrator™-5 | 5 µg | 50 |
