Product Description
LIGHT LABS is proud to offer the Zymo Research DNA Clean & Concentrator™-500 (Cat # D4032) — the highest capacity kit in Zymo Research's industry-leading DNA Clean & Concentrator™ (DCC) product line. Designed for the rapid, large-format purification and concentration of up to 500 µg of high-quality DNA in a single column, the DCC-500 is the go-to solution for researchers working with large-scale restriction endonuclease digestions, crude DNA preparations, and other high-input DNA workflows where standard cleanup kits fall short.
Using Zymo Research's Zymo-Spin™ VI column technology, the DCC-500 delivers ultra-pure DNA (A260/280 ≥ 1.8) in less than 20 minutes. The unique large-format column construction allows sample loading and washing to be performed using either a centrifuge, microcentrifuge, or vacuum source — providing flexibility for a wide range of lab setups. Purified DNA is ideally suited for PCR, DNA sequencing, DNA transfection, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, and microarray applications. LIGHT LABS is your trusted source for Zymo Research products.
KEY FEATURES
- Highest capacity DNA cleanup kit in the Zymo Research DCC line — processes up to 500 µg of DNA per column
- Complete purification and concentration in less than 20 minutes
- High DNA purity — A260/280 ≥ 1.8, ideal for downstream applications requiring ultra-clean DNA
- DNA size range: ~50 bp to 23 kb
- Unique Zymo-Spin™ VI large-format column compatible with centrifuge, microcentrifuge, or vacuum source for flexible processing
- Effectively removes primers, nucleotides, enzymes, salts, and other enzymatic reaction impurities
- Eluted DNA is immediately ready for use in PCR, sequencing, ligation, transfection, restriction digestion, RNA transcription, radiolabeling, and microarray applications
- Simple bind-wash-elute workflow with no organic solvents required
- Kit includes DNA Binding Buffer, DNA Wash Buffer, DNA Elution Buffer, and Zymo-Spin™ VI Columns
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | D4032 |
| Brand | Zymo Research |
| Kit Name | DNA Clean & Concentrator™-500 (DCC-500) |
| Number of Preps | 20 |
| Maximum DNA Binding Capacity | 500 µg |
| DNA Size Range | ~50 bp to 23 kb |
| DNA Purity | A260/280 ≥ 1.8 |
| Column Type | Zymo-Spin™ VI (large format) |
| Processing Time | Less than 20 minutes |
| Processing Method | Centrifuge, microcentrifuge, or vacuum |
| Elution Volume | Minimum 1 mL |
| Storage Temperature | Room temperature |
| Kit Components | DNA Binding Buffer (2 x 100 mL), DNA Wash Buffer (48 mL), DNA Elution Buffer (2 x 50 mL), Zymo-Spin™ VI Columns (20), Instruction Manual |
APPLICATIONS
- Large-scale PCR product cleanup and concentration
- Large-scale restriction endonuclease digestion cleanup
- Crude DNA preparation purification
- DNA sequencing sample preparation
- DNA transfection and ligation
- RNA transcription template preparation
- Radiolabeling reaction cleanup
- Microarray and hybridization sample preparation
- Any application requiring high-capacity, rapid DNA purification from large-volume or high-input samples
ORDERING INFORMATION
| Cat # | Description | Max Capacity | Preps |
|---|---|---|---|
| D4032 | DNA Clean & Concentrator™-500 | 500 µg | 20 |
| D4031 | DNA Clean & Concentrator™-500 | 500 µg | 10 |
| D4014 | DNA Clean & Concentrator™-100 | 100 µg | 25 |
| D4013 | DNA Clean & Concentrator™-100 | 100 µg | 10 |
| D4004 | DNA Clean & Concentrator™-25 | 25 µg | 50 |
| D4003 | DNA Clean & Concentrator™-25 | 25 µg | 25 |
| D4034 | DNA Clean & Concentrator™-5 | 5 µg | 50 |
| D4033 | DNA Clean & Concentrator™-5 | 5 µg | 25 |
