Zymo Research ZR-96 Oligo Clean & Concentrator™ D4062 | 2×96 Prep Plate Format, Oligonucleotide Cleanup ≥16 nt

The Zymo Research ZR-96 Oligo Clean & Concentrator™ D4062 is a 2×96-preparation 96-well plate format oligonucleotide cleanup kit — providing rapid, high-throughput purification of oligonucleotides ≥16 nt from enzymatic reactions using Zymo-Spin™ I-96 Plate technology. There is no need for organic denaturants or chloroform. The kit features Zymo-Spin plate technology and employs a single-buffer system that allows for efficient oligonucleotide adsorption to the matrix of each well in the Zymo-Spin™ I-96 Plate. Oligonucleotide is washed and concentrated into a small volume of water (≥10 µl). Purified oligonucleotide is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc. Effectively removes unincorporated nucleotides, short oligos, dyes, enzymes, and salts. >90% recovery of ssDNA oligonucleotides. 2 plates (2×96 preps) — 192 total wells. Catalog # D4062. The 4×96-preparation version is available as D4063.

D4062 vs. D4063 — Choosing the Right Kit Size: The D4062 and D4063 are identical ZR-96 Oligo Clean & Concentrator™ kits in every way — the number of plates and reagent volumes are the sole differences:

The D4062 is the preferred choice for labs with moderate oligonucleotide cleanup throughput — processing up to 192 samples per kit — while the D4063 is suited for core facilities and high-volume synthesis labs processing 384 samples per kit. For labs evaluating the ZR-96 Oligo Clean & Concentrator™ platform for the first time, the D4062 provides a lower-commitment entry point before committing to the larger D4063 format.

96-Well Plate Format — 96 Samples Per Plate, 192 Per Kit The defining format distinction of the D4062 is its 96-well plate architecture — processing up to 96 oligonucleotide samples simultaneously per plate, 2 plates per kit, 192 total samples per kit. The plate format is specifically designed for moderate-throughput oligonucleotide synthesis labs, core laboratories, and any workflow where individual spin column cleanup of many samples simultaneously would be prohibitively time-consuming. For oligo purification workflows requiring parallel processing of dozens to nearly 200 samples, the D4062 96-well plate format provides the ideal balance of throughput and kit economy.

Oligonucleotide Cleanup ≥16 nt — Size Limits and Compatibility Size Limits — For oligonucleotides ≥16 nt, up to 23 kb. The ZR-96 Oligo Clean & Concentrator™ recovers oligonucleotides from 16 nt — the minimum length reliably retained on the Zymo-Spin I-96 plate matrix — through large DNA up to 23 kb. Compatible sample sources include enzymatic reaction mixtures containing oligonucleotides ≥16 nt including:

• Oligonucleotide synthesis reactions — removal of failed sequences, protecting groups, and synthesis byproducts
• Radioactively labeled oligos — ³²P, ³³P, and ³⁵S-labeled oligonucleotide probes
• Biotin-labeled oligonucleotides — biotinylated probes and primers
• DIG-labeled oligonucleotides — digoxigenin-labeled probes
• Fluorescently labeled oligos — removal of unincorporated fluorescent dyes
• PCR primers — cleanup from enzymatic reactions
• Any oligo-containing enzymatic reaction — enzymes, salts, unincorporated nucleotides, and short fragments removed

>90% Recovery — High-Efficiency Oligonucleotide Purification >90% recovery of ssDNA oligonucleotides — maintained consistently across all 96 wells of each plate and across both plates in the D4062 kit. For oligonucleotide synthesis and labeling workflows where oligo yield and recovery directly affect downstream assay performance, the consistently high recovery of the D4062 ensures maximum oligo yield from every purification across all 192 samples per kit.

10µg ssDNA/RNA or 5µg dsDNA Per Well — Per-Well Binding Capacity Binding Capacity — 10 µg ssDNA/RNA or 5 µg dsDNA per well. The per-well binding capacity accommodates the full range of standard oligonucleotide reaction scales — from small-scale single-tube synthesis reactions to moderate-scale parallel synthesis in 96-well format across both plates of the D4062.

Single-Buffer System — Simple, Rapid, No Organic Solvents There is no need for organic denaturants or chloroform. The kit employs a single-buffer system that allows for efficient oligonucleotide adsorption — a single Oligo Binding Buffer addition to the sample initiates binding, followed by wash and elution. No organic solvent handling, no hazardous waste, and no pH adjustment steps are required.

≥10µL Elution — Concentrated Oligonucleotide Recovery Elution Volume — ≥10 µl. Oligonucleotide is washed and concentrated into a small volume of water (≥10 µl) — compatible with direct use in downstream hybridization, PCR, ligation, sequencing, and microarray applications without further concentration steps.

High Purity — A260/A280 and A260/A230 >1.8 Purity — A260/A280 & A260/A230 >1.8. Purified oligonucleotide is ready for hybridization, sequencing, ligation, PCR, and all other downstream molecular biology applications.

Complete Plate System Included Collection Plates: 4 included. Elution Plates: 2 included. 96-Well Cover Foil: 2 included. The D4062 includes all plates required for the complete cleanup workflow — Zymo-Spin I-96 binding plates, collection plates for flow-through capture, elution plates for eluate collection, and cover foil for plate sealing.

Room Temperature Storage — All Kit Components Store all kit components at room temperature. Note: Ethanol must be added to DNA Wash Buffer concentrate prior to use as indicated on the label.

Complete Kit Contents — D4062 (2×96 Preps):

Complete ZR-96 Oligo Clean & Concentrator™ Family:

Compatible Replacement — Cross-Reference Guide

The Zymo Research ZR-96 Oligo Clean & Concentrator™ D4062 is a comparable alternative to 96-well plate format oligonucleotide cleanup kits from several major manufacturers:

• Qiagen QIAquick 96 PCR Purification Kit (28181) — 96-well format DNA cleanup
• NEB Monarch PCR & DNA Cleanup Kit — spin column format (no 96-well equivalent)
• Macherey-Nagel NucleoSpin 96 PCR — 96-well plate PCR cleanup format

The D4062 specifically targets oligonucleotides ≥16 nt in a 96-well plate format — more specialized than standard PCR cleanup plates. These products may differ in protocol, yield, and performance characteristics.

Key Features

• Zymo Research ZR-96 Oligo Clean & Concentrator™ — Catalog # D4062
• 2×96 prep plate format — 192 total wells, 2 Zymo-Spin™ I-96 Plates
• High-throughput oligonucleotide cleanup — 96 samples simultaneously per plate
• Oligonucleotide size range: ≥16 nt to 23 kb
• Binding capacity: 10µg ssDNA/RNA or 5µg dsDNA per well
• >90% recovery of ssDNA oligonucleotides
• ≥10µL minimum elution volume
• Single-buffer system — no organic solvents, no chloroform
• Effectively removes unincorporated nucleotides, short oligos, dyes, enzymes, and salts
• Compatible with radioactive-, biotin-, DIG-, and fluorescent-labeled oligonucleotides
• A260/A280 and A260/A230 >1.8 — high-purity eluate
• Complete plate system included — binding plates, collection plates, elution plates, cover foil
• Room temperature storage — all components
• Ethanol must be added to DNA Wash Buffer concentrate before use
• 1-year kit component integrity guarantee
• 4×96 prep version: D4063

Supported Applications Moderate-throughput oligonucleotide synthesis cleanup, parallel purification of radioactively labeled probes, biotin-labeled oligonucleotide cleanup, DIG-labeled probe purification, fluorescent label removal from labeled oligos, PCR primer cleanup from enzymatic reactions, microarray probe cleanup, hybridization probe preparation, sequencing primer cleanup, and any molecular biology workflow requiring simultaneous purification of up to 96 oligonucleotide samples ≥16 nt per plate in a 96-well format.

Technical Specifications