CELLTREAT® 229351 182cm² Tissue Culture Flask with Vented Cap, Sterile — Case of 40

The CELLTREAT® 229351 is a 182cm² tissue culture treated flask with vented cap — sterile, 600mL total volume, and manufactured to the most stringent cleanroom and quality standards in the industry. The 182cm² flask offers more surface area compared to the traditional 175cm² (T175) flask — providing 4% additional growth area in the same footprint, allowing labs to culture more cells per flask with identical handling and incubator space. CELLTREAT Tissue Culture flasks are manufactured under the most stringent conditions and include a 100% integrity test to assure the reliability with your tissue and cell culture work. Class 100,000 clean room, USP Class VI virgin polystyrene, and ISO 9001:2000 quality management systems all contribute to the consistent tissue and cell culture performance. Sold as a case of 40 sterile, individually packaged flasks.

182cm² — More Growth Area Than the Standard T175 The 182cm² flask offers more surface area compared to the traditional 175cm² (T175) flask. While most manufacturers produce flasks with a 175cm² growth area, CELLTREAT's 229351 delivers 182cm² — a meaningful increase in usable growth surface for labs that need to maximize cell yield per flask. For cell lines with high media consumption, the extra surface area of the 229351 vs. a standard T175 translates directly to more cells per passage without requiring an additional flask format in the lab inventory.

Negatively Charged Hydrophilic TC Treatment — Uniform, Consistent Cell Attachment The tissue culture surface treatment used for CELLTREAT products provides a negatively charged hydrophilic surface and results in a consistent cell attachment surface. Growth surfaces are flat and free from striation to maximize usable growth area and transparency. The uniform hydrophilic TC treatment of the 229351 promotes consistent, reproducible cell attachment across the entire 182cm² growth surface — eliminating the uneven attachment and growth patterns that can result from inconsistent surface treatment processes. The flat, striation-free growth surface maximizes effective growth area and provides clear optical transparency for direct microscopic observation of cell morphology and confluency.

100% Integrity Tested — Every Flask, Every Lot CELLTREAT Tissue Culture flasks include a 100% integrity test to assure the reliability with your tissue and cell culture work. Every single 229351 flask is individually tested for sterile integrity before packaging — not batch-sampled, not statistically tested, but 100% tested. This zero-tolerance quality control standard ensures that no compromised flask reaches your lab, protecting cell culture experiments from unexpected contamination events caused by flask seal failures.

Large Opening Canted Neck — Easy Access for Pipettes and Cell Scrapers Large opening, canted neck design and moderate angles allow easy access to the entire flask surface — ideal for cell scrapers. The large-opening canted neck of the 229351 is a premium design feature that distinguishes it from standard T175 flasks. The large opening accommodates wide-bore pipettes and cell scrapers without the access restrictions of narrower-neck flask designs, allowing the operator to reach the entire growth surface cleanly and completely. The moderate canted angle provides ergonomic viewing and handling at the biosafety cabinet while maintaining stable positioning on the benchtop and in the incubator.

Stable Base — Fill Flask Upright Without Tipping The stable base enables filling with the flask standing upright. The 229351's wide, stable base allows media addition with the flask in the upright position — eliminating the need to tilt or hold the flask during filling and reducing the risk of spills and cross-contamination during media changes in the biosafety cabinet.

Thick, Durable Walls — Mechanical Protection for High-Value Cultures Thick, durable walls provide mechanical protection against cracking, impact damage, and flex deformation during handling, incubation, and centrifugation — protecting high-value cell cultures from unexpected flask failure. The thick-wall construction is particularly important for large-format flasks where the greater volume and weight of media creates higher mechanical stress during routine handling.

Molded Interlocking T Design — Improved Stacking Stability Molded, interlocking T design for improved stacking on 75, 182, & 300cm² and 250, 600 & 850mL size flasks. The interlocking T design molded into the base and cap of the 229351 creates a positive mechanical interlock between stacked flasks — preventing sliding, tipping, and collapse of flask stacks in the incubator. Compatible stacking with the 75cm², 182cm², and 300cm² flask formats allows mixed-format stacks without compromising stability.

Vented Cap with 0.22µm Hydrophobic Membrane — Passive Gas Exchange Vent caps feature a 0.22µm hydrophobic membrane. The integrated 0.22µm hydrophobic membrane in the vented screw cap allows passive CO&sub2; and O&sub2; exchange between the flask interior and the incubator atmosphere — eliminating the need to loosen caps for gas exchange and the contamination risk that accompanies the loosened-cap method. The hydrophobic membrane prevents aqueous media from being drawn through the filter under normal handling conditions.

Zip-Closure Bag Packaging — Convenient, Practical, Sterile Convenient zip-closure bag is a practical alternative to folding and taping open bags. The 229351 is packaged in a zip-closure resealable bag — a significant practical improvement over standard open-bag packaging that requires folding and taping to maintain sterility after the first flask is removed. The zip-closure design allows each subsequent flask to be removed cleanly and quickly while maintaining the sterile barrier for all remaining flasks in the bag.

Lot Number and Expiration Date Printed on Every Flask The flask is printed with lot number and expiration date for easy identification and tracking. Every 229351 flask is individually printed with the production lot number and expiration date — supporting full traceability and GMP documentation requirements in regulated cell culture environments, clinical applications, and quality-controlled research settings.

Class 100,000 Cleanroom — USP Class VI Virgin Polystyrene Class 100,000 clean room, USP Class VI virgin polystyrene, and ISO 9001:2000 quality management systems all contribute to the consistent tissue and cell culture performance. Every 229351 is manufactured in a certified Class 100,000 cleanroom from USP Class VI virgin polystyrene — the highest material purity standard for medical-grade plastics, ensuring freedom from extractables, leachables, and processing aids that could affect cell viability, proliferation, or experimental outcomes.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229351 is a direct compatible replacement for the following 175cm² and 182cm² tissue culture flasks from other manufacturers:

• Thermo Fisher Nunc™ 178883 — Nunc EasYFlask 175cm², vented cap
• Greiner Bio-One 661175 — T-175 Cell Culture Flask, vented filter cap

Note: The CELLTREAT 229351 delivers 182cm² — 4% more surface area than standard T175 flasks. Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete CELLTREAT® Tissue Culture Flask Family — Key Formats:

Key Features

• 182cm² growth area — more surface area than standard 175cm² (T175) flasks
• 100% integrity tested — every flask, every lot, no exceptions
• Negatively charged hydrophilic TC treatment — uniform, consistent cell attachment
• Flat, striation-free growth surface — maximizes usable growth area and transparency
• Large opening canted neck — easy pipette and cell scraper access to entire flask surface
• Stable base — fill flask standing upright without tipping
• Thick, durable walls — mechanical protection for high-value cultures
• Molded interlocking T design — improved stacking with 75, 182, and 300cm² flasks
• Vented cap with 0.22µm hydrophobic membrane — passive gas exchange, sterility maintained
• Zip-closure bag packaging — practical resealable alternative to fold-and-tape bags
• Lot number and expiration date printed on every flask
• Class 100,000 cleanroom manufactured
• USP Class VI virgin polystyrene
• ISO 9001:2000 quality management system
• Sterile — gamma irradiation
• Total volume: 600mL
• Case of 40 flasks

Supported Applications Anchorage-dependent cell line maintenance and expansion, large-scale cell culture for protein expression, viral vector production, vaccine manufacturing, cell banking, large-volume primary cell culture, stem cell expansion, scale-up from T-75 to T-182 to T-300, and any mammalian cell culture application requiring a large-format 182cm² vented tissue culture flask with premium design features and 100% integrity assurance.

Technical Specifications