Product Description
LIGHT LABS is proud to offer the CELLTREAT 225cm² Tissue Culture Treated Flask with Vent Cap (Cat # 229371) — a premium, sterile, TC-treated tissue culture flask providing a generous 225cm² of cell growth area for large-scale adherent mammalian cell culture. The 225cm² format fills the space between the standard 182cm² (229351/229381) and 300cm² (229361) flask sizes — making it the ideal choice for labs scaling up cell production beyond a standard T-175 or T-182 flask without yet requiring the full footprint of a T-300 format.
The tissue culture treated surface delivers a consistent, uniform hydrophilic surface optimized for maximum adherent cell attachment and spreading across the full 225cm² growth area. The large opening canted neck design with moderate angles provides easy access to the entire flask surface — particularly important for cell scraping and media changes in larger flask formats. The stable flat base allows the flask to stand upright during filling. The green HDPE vent cap with 0.22µm hydrophobic membrane maintains sterility while allowing consistent gas exchange with the CO₂ incubator atmosphere. The molded interlocking T-design on the flask base enables secure, space-efficient stacking with other compatible CELLTREAT flask sizes.
Every flask is printed with lot number and expiration date for quality traceability, and flasks are packaged in convenient zip-closure resealable bags. LIGHT LABS is your trusted source for CELLTREAT laboratory plasticware.
KEY FEATURES
- 225cm² TC-treated growth area — larger than standard T-175/T-182 formats; ideal scale-up flask between 182cm² and 300cm² formats
- Tissue culture treated (TC-treated) surface — uniform hydrophilic surface for consistent, maximum adherent cell attachment
- Vent cap with 0.22µm hydrophobic membrane — allows CO₂/gas exchange with incubator atmosphere while maintaining sterility; green HDPE cap for easy vent cap identification
- Large opening, canted neck design with moderate angles — easy access to entire flask surface with pipettes and cell scrapers
- Stable flat base — flask stands upright during filling without a stand or holder
- Molded interlocking T-design — compatible secure stacking with other CELLTREAT 75, 182, and 300cm² flasks and 250, 600, and 850mL flasks
- Thick, durable polystyrene walls for structural integrity
- Every flask printed with lot number and expiration date for easy identification and quality traceability
- Convenient zip-closure resealable bag packaging — practical alternative to folding and taping open bags
- Sterile — gamma irradiated
- Certified nonpyrogenic and RNase-/DNase-free
- Packaged 5 flasks per resealable bag; 5 bags per case (25 flasks/case)
- Fisher Scientific Catalog No. 50-114-9067
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 229371 |
| Fisher Scientific Catalog No. | 50-114-9067 |
| Brand | CELLTREAT Scientific Products |
| Product Name | 225cm² TC-Treated Tissue Culture Flask with Vent Cap |
| Growth Area | 225 cm² |
| Surface Treatment | Tissue Culture Treated (TC-Treated) |
| Cap Type | Vent Cap (0.22µm hydrophobic membrane) |
| Cap Color | Green HDPE |
| Neck Design | Canted neck, large opening, moderate angle |
| Base | Stable flat base (stands upright) |
| Stacking Feature | Molded interlocking T-design |
| Material | Medical-grade polystyrene (clear); Green HDPE (cap) |
| Sterility | Sterile (gamma irradiated) |
| Certifications | Nonpyrogenic, RNase-free, DNase-free |
| Lot/Expiry Printing | Yes — lot number and expiration date printed on each flask |
| Packaging | 5 flasks/resealable zip-closure bag; 5 bags/case |
| Quantity | 25 flasks/case |
APPLICATIONS
- Large-scale mammalian cell line maintenance and passaging
- Scale-up cell production between T-182 and T-300 flask formats
- Protein expression in adherent cell systems
- Viral vector (lentiviral, AAV, adenoviral) production in adherent cell lines
- Cell banking and cryopreservation requiring large cell numbers
- Large-scale transfection and stable cell line generation
- Primary cell culture requiring large growth area
- Any adherent cell culture application requiring a TC-treated 225cm² flask with vent cap
