Product Description
LIGHT LABS is proud to offer the CELLTREAT 75cm² Tissue Culture Treated Flask with Vent Cap (Cat # 229341) — the workhorse T-75 tissue culture flask, providing the ideal balance of growth area, media volume, and cell yield for routine mammalian cell line maintenance in research and production laboratories. With 75cm² of TC-treated growth area and a 10–20 mL working volume at a maximum volume of 250 mL, the 229341 is one of the most widely used flask sizes in cell biology — large enough for meaningful cell yields while remaining easy to handle in a biosafety cabinet.
The CELLTREAT 229341 features thicker, more durable polystyrene walls than many competing T-75 formats — providing superior structural integrity, reduced flex during handling, and improved resistance to warping. The tissue culture treated surface delivers a consistent, uniform hydrophilic surface optimized for maximum adherent cell attachment and spreading across the full 75cm² growth area — manufactured under class 100,000 cleanroom conditions using USP Class VI virgin polystyrene. The molded interlocking T-design enables secure, space-efficient stacking with other compatible CELLTREAT flask sizes (75, 182, and 300cm², and 250, 600, and 850mL formats). The large opening canted neck design with moderate angles provides easy access to the entire flask surface for pipettes and cell scrapers. Every flask is printed with lot number and expiration date for quality traceability and is packaged in convenient zip-closure resealable bags. LIGHT LABS is your trusted source for CELLTREAT laboratory plasticware.
KEY FEATURES
- 75cm² TC-treated growth area — classic T-75 format, one of the most widely used tissue culture flask sizes in cell biology research worldwide
- 10–20 mL working volume; 250 mL maximum volume
- Thicker, more durable polystyrene walls than many competing T-75 formats — superior structural integrity and reduced flex during handling
- Tissue culture treated (TC-treated) surface — uniform hydrophilic surface for consistent, maximum adherent cell attachment
- Manufactured under class 100,000 cleanroom conditions using USP Class VI virgin polystyrene
- 100% flask integrity tested during manufacturing for leak-free reliability
- Vent cap with 0.22µm hydrophobic membrane — allows CO₂/gas exchange with incubator atmosphere while maintaining sterility; green HDPE cap for easy vent cap identification
- Molded interlocking T-design — compatible secure stacking with CELLTREAT 75, 182, and 300cm² flasks and 250, 600, and 850mL flasks
- Large opening, canted neck design with moderate angles — easy access to entire flask surface with pipettes and cell scrapers
- Stable flat base — flask stands upright during filling without a stand or holder
- Every flask printed with lot number and expiration date for quality traceability
- Convenient zip-closure resealable bag packaging
- Sterile — gamma irradiated
- Certified nonpyrogenic and RNase-/DNase-free
- Packaged 5 flasks per resealable bag; 20 bags per case (100 flasks/case)
- Fisher Scientific Catalog No. 50-202-080
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 229341 |
| Fisher Scientific Catalog No. | 50-202-080 |
| Brand | CELLTREAT Scientific Products |
| Product Name | 75cm² TC-Treated Tissue Culture Flask with Vent Cap |
| Growth Area | 75 cm² |
| Maximum Volume | 250 mL |
| Working Volume | 10–20 mL |
| Surface Treatment | Tissue Culture Treated (TC-Treated) |
| Cap Type | Vent Cap (0.22µm hydrophobic membrane) |
| Cap Color | Green HDPE |
| Neck Design | Canted neck, large opening, moderate angle |
| Base | Stable flat base (stands upright) |
| Stacking Feature | Molded interlocking T-design |
| Material | USP Class VI virgin polystyrene (clear); Green HDPE (cap) |
| Manufacturing Environment | Class 100,000 cleanroom |
| Integrity Testing | 100% tested |
| Sterility | Sterile (gamma irradiated) |
| Certifications | Nonpyrogenic, RNase-free, DNase-free |
| Lot/Expiry Printing | Yes — lot number and expiration date printed on each flask |
| Packaging | 5 flasks/resealable zip-closure bag; 20 bags/case |
| Quantity | 100 flasks/case |
APPLICATIONS
- Routine mammalian cell line maintenance and passaging
- Medium-scale protein expression in adherent cell systems
- Viral vector (lentiviral, AAV, adenoviral) production at medium scale
- Cell banking and cryopreservation
- Drug screening and dose-response studies in adherent cells
- Transfection experiments at medium scale
- Primary cell culture requiring substantial growth area
- Scale-up from T-25 format before transitioning to T-150/T-182 formats
- Teaching and research labs running routine cell culture workflows
- Any adherent cell culture application requiring a TC-treated T-75 format flask
