Excel Scientific ThermalSeal® RT2™ TS-RT2-100 Polyester qPCR Sealing Film for Flat-Top Plates, Non-Sterile — 100/Pack

The Excel Scientific ThermalSeal® RT2™ TS-RT2-100 is a non-sterile, optically clear polyester sealing film for real-time qPCR specifically designed for flat-top and hard-shell dual-component PCR plates — delivering ultra-high optical clarity, a strong ultra-smooth highly conformable acrylic adhesive, and an operating temperature range of -40°C to +120°C. ThermalSeal® RT2™ film seals provide ultra-high optical clarity and a strong, ultra-smooth highly conformable adhesive, making them ideal for use with flat-top and hard-shell dual-component polycarbonate and/or polypropylene PCR plates. Also designed for use with standard 96-well plates where rows must be selectively protected or accessed. Certified DNase-free and RNase-free. Dimensions: 79.4 × 142.9 mm. 100 films per pack.

ThermalSeal® RT2™ vs. ThermalSeal® RT2RR™ — Flat-Top vs. Raised-Rim: Choosing the Right Film The TS-RT2-100 and TS-RT2RR-100 use identical film and adhesive technology but are dimensioned for different plate formats — choosing the correct version for your plate type is essential for optimal sealing performance:

Ultra-High Optical Clarity — Reproducible qPCR Detection ThermalSeal® RT2™ film seals provide ultra-high optical clarity — the same consistent optical standard as Excel's full ThermalSeal® RT™ sealing film line — making possible more reproducible, reliable, and consistent DNA amplification measurements. The 50µm polyester film provides exceptional optical transmission with minimal autofluorescence, critical for real-time qPCR where background signal from the sealing film directly affects fluorescence detection sensitivity and quantification accuracy. Consistent optical properties across every film in the 100-pack ensure run-to-run reproducibility in high-throughput qPCR environments.

Strong Ultra-Smooth Highly Conformable Adhesive — Ideal for Hard-Shell Plates The 25µm ultra-smooth, highly conformable acrylic adhesive of the ThermalSeal® RT2™ line is specifically engineered for the geometry of flat-top and hard-shell dual-component plates — conforming precisely to the well openings and plate surface for a reliable, consistent seal that prevents evaporation and contamination throughout qPCR thermal cycling. The ultra-smooth adhesive surface and highly conformable nature make it particularly effective on the polycarbonate and polypropylene surfaces of hard-shell PCR plates where standard adhesive films may not conform adequately.

Designed for Selective Row Access — Partial Plate Workflows ThermalSeal® RT2™ films are designed for use with standard 96-well plates where rows must be selectively protected or accessed — making the TS-RT2-100 an ideal choice for labs running partial-plate workflows, staged sample loading, or any protocol requiring individual row access while protecting sealed rows from evaporation and contamination. This selective access capability is a key advantage of the RT2™ film format over pressure-sensitive adhesive films that cannot be partially peeled and resealed.

Flat-Top and Hard-Shell Dual-Component Plate Compatibility The TS-RT2-100 is ideal for use with flat-top and hard-shell dual-component plates — mostly polycarbonate and/or polypropylene PCR plates. These plate types include the most commonly used hard-shell qPCR plates from Applied Biosystems, Bio-Rad, Roche, and other major PCR platform manufacturers. The RT2™ film's conformable adhesive is specifically matched to the surface geometry and material properties of these plate types for optimal sealing performance.

-40°C to +120°C Temperature Range — Full qPCR Workflow Coverage The operating temperature range of -40°C to +120°C covers the full PCR thermal cycling envelope — from initial denaturation through extension steps and cold storage — in a single film without requiring separate seals for different workflow stages.

100-Film Pack — High-Volume Format for qPCR Labs and Core Facilities At 100 films per pack, the TS-RT2-100 is the high-volume format designed for qPCR labs, genomics cores, and sequencing facilities running large numbers of plates per day. The 100-pack delivers the lowest per-film cost in the ThermalSeal® RT2™ line and provides consistent, reliable sealing performance across high-throughput qPCR workflows.

Certified DNase-Free and RNase-Free The TS-RT2-100 is certified DNase-free and RNase-free — meeting the biological purity standards required for sensitive molecular biology and qPCR applications where nuclease contamination from the sealing film could compromise amplification results.

Cross-Reference — Compatible Replacement For: The ThermalSeal® RT2™ film is widely used as a direct compatible replacement for the following products:

• Applied Biosystems® MicroAmp® Optical Adhesive Film (catalog # 4311971 / 4360954)
• Thermo Fisher Scientific MicroAmp® Optical Adhesive Film

Complete Excel Scientific ThermalSeal® Sealing Film Family — Key Formats:

Key Features

• Ultra-high optical clarity — consistent, reproducible qPCR fluorescence detection
• 50µm polyester film — strong, optically clear, temperature-resistant
• 25µm ultra-smooth, highly conformable acrylic adhesive
• Designed for flat-top and hard-shell dual-component polycarbonate/polypropylene PCR plates
• Suitable for standard 96-well plates requiring selective row access
• Dimensions: 79.4 × 142.9 mm (with tabs)
• Adhesive thickness: 25µm
• Temperature range: -40°C to +120°C
• Certified DNase-free and RNase-free
• Non-sterile
• 100 films per pack — lowest per-film cost in the RT2™ line
• Shelf life: 36 months
• Made in the USA

Supported Applications Real-time qPCR with flat-top and hard-shell dual-component PCR plates, endpoint PCR, partial-plate workflows requiring selective row access, high-throughput qPCR in core facilities, and any flat-top 96-well plate workflow requiring ultra-high optical clarity sealing film with a strong, conformable, temperature-resistant seal.

Technical Specifications