Excel Scientific ThermalSeal® RT2RR™ TS-RT2RR-100 Polyester qPCR Sealing Film for Raised-Rim Plates, Non-Sterile — 100/Pack

Product Description

The Excel Scientific ThermalSeal® RT2RR™ TS-RT2RR-100 is a non-sterile, optically clear polyester sealing film for real-time qPCR specifically designed to fit within the edges of raised-rim 96-well plates — delivering ultra-high optical clarity, a strong inert temperature-resistant adhesive, and an operating temperature range of -40°C to +120°C. ThermalSeal RT2RR 50µm polyester sealing films for real-time PCR are sized to fit within the edges of raised-rim 96-well plates. The same consistent ultra-high optical clarity as Excel's ThermalSeal RT™ sealing films makes possible more reproducible, reliable, and consistent DNA amplification measurements. Certified DNase-free, RNase-free, and nucleic acid-free. Recommended for specific DNA sequence detection in clinical diagnostics, forensic science and basic research. 100 films per pack.

ThermalSeal® RT2RR™ vs. ThermalSeal® RTS™ — Understanding the Difference The Excel Scientific ThermalSeal® sealing film lineup includes two distinct film technologies — and choosing the right one for your plate format is critical for optimal sealing performance:

• ThermalSeal® RTS™ (TSS-RTQ / TSS-RTQS series): Uses a 50µm polyolefin film with 50µm encapsulated silicone adhesive. The encapsulated silicone is released only at well-sealing zones, remaining inert over sample wells. Best for qPCR, storage, and protein crystallization.
• ThermalSeal® RT2RR™ (TS-RT2RR-100): Uses a 50µm polyester film with a 25µm ultra-smooth, highly conformable acrylic adhesive. Specifically cut and dimensioned to fit within the raised rim of standard 96-well plates. Ideal for flat-top and hard-shell dual-component polycarbonate/polypropylene PCR plates in raised-rim format. Best for qPCR, clinical diagnostics, and forensic science applications.

The RT2RR designation specifically indicates this film is the raised-rim (RR) cut of the RT2 film — dimensioned at 77.8 × 130.8mm to fit within the raised rim of standard 96-well plates, whereas the standard TS-RT2-100 (79.4 × 142.9mm) is sized for flat-top plates.

Ultra-High Optical Clarity — Reproducible qPCR Detection The consistent ultra-high optical clarity of ThermalSeal RT2RR™ makes possible more reproducible, reliable, and consistent DNA amplification measurements. The 50µm polyester film provides exceptional optical transmission with minimal autofluorescence — critical for real-time qPCR where background signal from the sealing film directly affects fluorescence detection sensitivity and quantification accuracy. Consistent optical properties across every film in the 100-pack ensure run-to-run reproducibility in high-throughput qPCR environments.

Fits Within Raised Plate Rim — Prevents Lifting and Evaporation ThermalSeal RT2RR™ films are sized to fit within the edges of raised-rim 96-well plates. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with sealing films that overlap the plate rim. The RT2RR dimensions (77.8 × 130.8mm with tabs; 118.1mm length with tabs removed, 45° corners) are precisely engineered for raised-rim plates — ensuring the film sits cleanly within the rim without overhang that causes film lifting, uneven sealing, or evaporation-driven well-to-well variability.

Two End Tabs — Precise Positioning Made Easy Two end tabs assist in positioning the sealing film. The end tabs allow the film to be accurately positioned on the plate before sealing pressure is applied — preventing misalignment and ensuring consistent well coverage across every plate. The perforated tab boundaries allow clean, easy removal after positioning without lifting or displacing the sealed film.

Strong, Inert, Temperature-Resistant Adhesive An inert, strong, temperature-resistant adhesive assures reliable sealing around each well. The 25µm ultra-smooth, highly conformable adhesive conforms precisely to the geometry of each well opening — forming a reliable, consistent seal that prevents evaporation and contamination throughout qPCR thermal cycling, cold storage, and all intermediate workflow steps.

-40°C to +120°C Temperature Range — Full qPCR Workflow Coverage The operating temperature range for this product is -40°C to +120°C. This wide temperature range covers the full PCR thermal cycling envelope — from denaturation steps at elevated temperatures through cold storage and freeze-thaw cycles — in a single film without requiring separate seals for different workflow stages.

Recommended for Clinical Diagnostics and Forensic Science Recommended for specific DNA sequence detection in clinical diagnostics, forensic science and basic research. The RT2RR™ film's ultra-high optical clarity, strong consistent seal, and certified nuclease-free status make it particularly suitable for regulated qPCR applications in clinical molecular diagnostics and forensic DNA analysis — where seal performance, optical reproducibility, and material certification directly affect result validity and defensibility.

Certified DNase-Free, RNase-Free, and Nucleic Acid-Free The TS-RT2RR-100 is certified DNase-free, RNase-free, and nucleic acid-free — meeting the biological purity standards required for sensitive molecular biology and clinical diagnostics applications where nuclease contamination from the sealing film would compromise amplification results.

Cross-Reference — Compatible Replacement For: The ThermalSeal® RT2RR™ film is widely used as a direct compatible replacement for the following products:

• Applied Biosystems® MicroAmp® Optical Adhesive Film (catalog # 4311971 / 4360954)
• Thermo Fisher Scientific MicroAmp® Optical Adhesive Film

Excel Scientific ThermalSeal® Sealing Film Family — Key Formats:

Key Features

• Ultra-high optical clarity — consistent, reproducible qPCR fluorescence detection
• 50µm polyester film — strong, optically clear, temperature-resistant
• 25µm inert acrylic adhesive — strong, conformable, temperature-resistant
• Specifically dimensioned for raised-rim 96-well plates (77.8 × 130.8mm with tabs)
• Fits within raised plate rim — prevents film lifting and evaporation
• Two end tabs with perforated boundaries for precise positioning
• 45° corners with end tabs removed — length 118.1mm
• Temperature range: -40°C to +120°C
• Certified DNase-free, RNase-free, and nucleic acid-free
• Recommended for clinical diagnostics, forensic science, and basic research
• Non-sterile
• 100 films per pack
• Shelf life: 36 months
• Made in the USA

Supported Applications Real-time qPCR with raised-rim 96-well plates, clinical molecular diagnostics, forensic DNA sequence detection, endpoint PCR, sample storage, and any raised-rim 96-well plate workflow requiring ultra-high optical clarity sealing film with a strong, inert, temperature-resistant seal.

Technical Specifications