Excel Scientific ThermalSeal® RTS™ TSS-RTQ-100 Optically Clear qPCR Sealing Film, Non-Sterile — 100/Pack

Product Description

The Excel Scientific ThermalSeal® RTS™ TSS-RTQ-100 is a non-sterile, optically clear microplate sealing film designed for real-time qPCR, sample storage, and protein crystallization applications — featuring the proven 50µm inert encapsulated silicone adhesive technology of the ThermalSeal® RTS™ line in the highest-volume non-sterile pack size available. The TSS-RTQ-100 is the most economical per-film format for high-throughput qPCR labs, core facilities, and any workflow requiring large quantities of optically clear sealing film without a sterility requirement. Certified DNase-free, RNase-free, and nucleic acid-free. Dimensions: 76.2 × 133.4 mm. 100 films per pack.

Encapsulated Silicone Adhesive — The Strongest Available Seal, Inert Over Wells The defining technology of ThermalSeal® RTS™ is its encapsulated silicone adhesive system. The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. Adhesive on non-sealing areas of the film — such as directly over sample wells — remains encapsulated and inert, eliminating any risk of adhesive-sample interaction, extractable contamination, or interference with fluorescence detection. This precision adhesive release mechanism is unique to ThermalSeal® RTS™ and is the primary reason the film forms a stronger, more consistent seal than standard pressure-sensitive adhesive films.

High Optical Clarity — Minimal Autofluorescence for Accurate qPCR Detection ThermalSeal® RTS™ films offer high optical clarity with minimal to no autofluorescence — critical for real-time qPCR and fluorescence-based detection where background signal from the sealing film directly affects assay sensitivity and result reproducibility. At 100 films per pack, the TSS-RTQ-100 provides consistent, uniform optical clarity across every film in the pack — ensuring reproducible run-to-run performance in high-throughput qPCR environments where plate-to-plate seal consistency directly affects data quality.

100-Film Pack — The High-Volume Format for qPCR Labs and Core Facilities The TSS-RTQ-100 is the largest pack size in the ThermalSeal® RTS™ non-sterile line — making it the natural choice for high-throughput qPCR labs, genomics cores, and sequencing facilities that run large numbers of plates per day and require a reliable, cost-efficient supply of optically clear sealing film. At 100 films per pack, the TSS-RTQ-100 delivers the lowest per-film cost in the non-sterile ThermalSeal® RTS™ line — providing meaningful reagent cost savings for labs where sealing film consumption is a significant budget line item.

Non-Sterile Format — For High-Throughput qPCR, Storage, and Crystallization The TSS-RTQ-100 is the non-sterile format of the ThermalSeal® RTS™ line — appropriate for the vast majority of qPCR, sample storage, and protein crystallization workflows where biological sterility of the sealing film is not required. For cell-based assays, viral work, and other workflows requiring sterile sealing materials, the TSS-RTQS-50 (sterile, 50/pack) is the appropriate choice.

Fits Within Raised Plate Rim — Prevents Film Lifting and Evaporation ThermalSeal® RTS™ films are sized to fit within the edges of raised-rim 96-well plates — preventing the film lifting and elevated evaporation rates that occur with films that overlap the plate rim. Two end tabs with perforated boundaries assist in precise film positioning on the plate, and clean tab removal after placement does not disturb the seal. With end tabs removed, the film length is 113.0mm — fitting cleanly within the raised rim of standard 96-well plates without overhang.

Non-Tacky Adhesive — Easy Handling, No Sticking to Gloves The non-tacky adhesive surface simplifies film handling before sealing — the film can be picked up, repositioned, and aligned on the plate without sticking to gloves or accumulating fingerprints that would compromise optical clarity. This practical advantage is particularly important in high-throughput environments where speed and consistency of plate sealing directly affect workflow productivity.

-80°C to +105°C Temperature Range — From Deep Freeze to Thermal Cycling The exceptional temperature range of -80°C to +105°C covers the full spectrum of microplate workflows in a single film — from ultra-low temperature sample storage at -80°C through qPCR and endpoint PCR thermal cycling at up to 105°C. A single pack covers every temperature stage of the workflow without requiring separate seal inventories for cold storage vs. thermal cycling applications.

Chemically Resistant — Including DMSO for HTS Applications The TSS-RTQ-100 is chemically inert with no extractables except at extreme pH, and is resistant to DMSO, acetonitrile, and methanol — suitable for compound library storage plates, high-throughput screening workflows, and any application involving chemically aggressive sample matrices. The DMSO resistance makes the TSS-RTQ-100 particularly well-suited for HTS labs running large numbers of compound library plates requiring sealing film at production volume.

Cross-Reference — Compatible Replacement For: The ThermalSeal® RTS™ film is widely used as a direct compatible replacement for the following products:

• Applied Biosystems® MicroAmp® Optical Adhesive Film (catalog # 4311971 / 4360954)
• Thermo Fisher Scientific MicroAmp® Optical Adhesive Film

Complete ThermalSeal® RTS™ Film Family — All Formats:

Key Features

• Optically clear 50µm polyolefin film
• 50µm inert encapsulated silicone adhesive — releases only at well-sealing zones
• Strongest available heat-resistant seal for evaporation prevention
• Adhesive remains inert over sample wells — no sample interference
• High optical clarity — minimal to no autofluorescence
• Fits within raised plate rim — prevents film lifting and evaporation
• Non-tacky adhesive — easy handling, no sticking to gloves
• Two end tabs with perforated boundaries for precise positioning
• Temperature range: -80°C to +105°C
• Chemically resistant — DMSO, acetonitrile, methanol
• Chemically inert — no extractables except at extreme pH
• Certified DNase-free, RNase-free, and nucleic acid-free
• Non-sterile
• Dimensions: 76.2 × 133.4 mm (with tabs); 76.2 × 113.0 mm (tabs removed)
• Shelf life: 36 months
• 100 films per pack — lowest per-film cost in the non-sterile ThermalSeal® RTS™ line
• Made in the USA

Supported Applications High-throughput real-time qPCR, endpoint PCR, sample storage at -80°C, protein crystallization, DMSO compound library storage (HTS), fluorescence-based microplate assays, genomics core facility plate sealing, sequencing reaction plate sealing, and any 96-well plate workflow requiring optically clear, chemically resistant sealing film at high volume where sterility is not required.

Technical Specifications