MTC Bio OptiWell™ D3096-29S | 96-Well Deep Well Plate, Round Flat Bottom, 1.2mL, Sterile — 50/Case (10 Bags of 5)

Product Description

The MTC Bio OptiWell™ D3096-29S is a 96-well deep well plate with round flat-bottom wells and 1.2mL capacity per well — sterile by gamma irradiation, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Sterilized by gamma irradiation. Case of 50 plates — 10 bags of 5 plates each.

Round Flat-Bottom Wells — The Preferred Format for Optical Detection and Absorbance Measurement The round flat-bottom well geometry of the D3096-29S is the preferred format for 96-well deep well applications requiring optical reading of well contents — including absorbance measurement, turbidity monitoring, fluorescence reading from above or below, and any workflow where the flat well base provides a consistent optical path through the sample for quantitative measurement. The flat bottom of each well creates a uniform liquid depth across the full well base area — ensuring consistent optical path length for all wells on the plate and eliminating the depth variation at the well base that affects absorbance readings in V-bottom and U-bottom formats. For applications such as bacterial growth monitoring by OD600, enzymatic assay kinetics at 1.2mL volume, and colorimetric assays in deep well format, the flat-bottom geometry of the D3096-29S delivers more consistent and accurate optical measurements than V-bottom or U-bottom alternatives.

1.2mL Per Well — The Mid-Range Deep Well Volume The 1.2mL per-well capacity of the D3096-29S sits between the standard 1.0mL format (D3096-15) and the large 2.2mL format (D3096-41S/43S) — providing a meaningful volume advantage over the 1.0mL format for bacterial culture applications requiring more growth medium volume, while maintaining a more compact plate height than the full 2.2mL deep well format. For bacterial culture workflows where 1.0mL is insufficient for overnight growth density but 2.2mL would leave excessive unused volume, the 1.2mL flat-bottom format of the D3096-29S provides the optimal volume for mid-scale bacterial culture, protein expression, and compound preparation workflows.

D3096-29S vs. D3096-15 — Flat Bottom vs. U-Bottom at 1.0–1.2mL Sterile:

D3096-29S in the Full OptiWell™ 96-Well Deep Well Family:

Sterile by Gamma Irradiation — Validated for Bacterial Culture and Sterile Workflows Sterile. Sterilized by gamma irradiation. The D3096-29S is sterilized by gamma irradiation — providing validated, reliable sterility without heat damage or residual chemical concerns. The "S" suffix designates the sterile format. Gamma irradiation sterilization qualifies the D3096-29S for bacterial culture, protein expression in E. coli, mammalian cell-based assays, and any sterile 96-well deep well workflow requiring a flat-bottom well geometry.

D3096-29S vs. D3096-29 — Sterile vs. Non-Sterile:

Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-29S conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, and other standard HTS robotic systems.

4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g. The robust polypropylene construction of the D3096-29S withstands centrifugal forces up to 4,000 × g — covering all standard plate centrifugation protocols for bacterial cell pelleting, sample concentration, and other 96-well deep well workflows.

Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Manufactured from virgin medical-grade polypropylene — free from recycled resin contaminants, processing aids, and extractables. Certified RNase/DNase free and non-pyrogenic.

Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns) allows instant identification of specific wells during liquid handling setup, plate reading, and data recording.

Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. All plates are certified non-pyrogenic and RNase/DNase free.

Compatible Replacement — Cross-Reference Guide

The MTC Bio OptiWell™ D3096-29S is a direct compatible replacement for sterile 96-well round flat-bottom 1.2mL deep well plates from several major manufacturers:

• Thermo Fisher AB-0859 — Thermo Scientific 96-well round flat-bottom deep well plate, 1.2mL, sterile
• Greiner Bio-One 780201 — 96-well deep well plate, round flat-bottom, 1.2mL, sterile
• Eppendorf® 0030503390 — Eppendorf 96-well deepwell plate, round flat-bottom, 1.2mL, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Key Features

• MTC Bio OptiWell™ — Catalog # D3096-29S
• 96-well deep well plate — round flat-bottom wells
• 1.2mL per-well capacity
• Round flat-bottom — optimal for optical detection, absorbance measurement, and OD reading
• Sterile — gamma irradiation
• Virgin medical-grade polypropylene — minimal binding and liquid retention
• Black printed alpha-numerics — instant well identification
• ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
• 4,000 × g centrifugation rating
• Compatible with silicone sealing mats and sealing film
• No lid, no skirt
• Class 100,000 cleanroom production
• Certified RNase-free and DNase-free
• Certified non-pyrogenic
• Sigma-Aldrich catalog # MTCD309629S
• Case of 50 plates — 10 bags of 5 plates

Supported Applications Bacterial growth monitoring by OD600 in 96-well deep well format, protein expression in E. coli at 1.2mL scale, enzymatic assay kinetics at mid-volume scale, colorimetric and turbidity assays in deep well format, cell-based assays requiring sterile flat-bottom deep well plates, compound preparation and dilution at 1.2mL scale, automated liquid handling workflows requiring flat-bottom optical reading capability, sterile sample storage at mid-volume scale, centrifugation workflows up to 4,000 × g, and any 96-well application requiring sterile round flat-bottom deep well format with 1.2mL per-well capacity and full automated platform compatibility.

Technical Specifications