Zymo Research RNA Clean & Concentrator™-5 R1013 | 50 Preps with DNase I Set, Zymo-Spin™ IC Columns

The Zymo Research RNA Clean & Concentrator™-5 R1013 is a 50-preparation RNA cleanup and concentration kit — providing a simple and reliable method for the rapid preparation of high-quality, RT-PCR-ready, DNA-free RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (>17 nt), large RNAs (>200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations. DNase I Set included. 50 preparations. Catalog # R1013. The 200-preparation version is available as R1014.

R1013 vs. R1014 vs. R1015 vs. R1016 — Understanding the RNA Clean & Concentrator™-5 Family:

The R1013 is the standard choice for labs requiring DNA-free RNA — the included DNase I Set allows in-column DNase digestion to eliminate genomic DNA contamination from RNA samples in a single integrated workflow step without a separate DNase treatment tube.

5-Minute RNA Cleanup — Ultra-Pure RNA Rapidly Quick, 5-minute recovery of ultra-pure RNA (≥17 nt) from enzymatic reactions, aqueous phase (following TRIzol® extraction) and other sources. High-quality RNA eluted in ≥6 µl is ready for reverse transcription, microarray, sequencing, etc. The 5-minute preparation time of the RNA Clean & Concentrator™-5 makes it one of the fastest RNA cleanup kits available — processing samples from crude RNA preparation to highly purified, concentrated eluate in the time it takes to run a single centrifugation cycle. For labs processing multiple samples simultaneously, the 5-minute protocol dramatically reduces total cleanup time compared to precipitation-based methods that require 30–60+ minutes of incubation and centrifugation.

DNase I Set Included — In-Column DNA Elimination The R1013 is supplied with the DNase I Set (E1010) — enabling optional in-column DNase digestion to eliminate genomic DNA contamination directly on the column during the cleanup workflow. Reconstitute lyophilized DNase I with DNase/RNase-Free Water and mix by gentle inversion. Store frozen aliquots. In-column DNase treatment is performed directly on the spin column matrix between the RNA binding and wash steps — adding a single additional incubation step without requiring a separate tube, additional centrifugation steps, or heat inactivation that could affect RNA integrity. The result is DNA-free RNA produced in the same 5-minute cleanup workflow with minimal additional handling. 

Selective RNA Recovery — Total RNA, Large RNA, or Small RNA A unique and distinctive feature of the RNA Clean & Concentrator™-5 is its ability to selectively recover different RNA size classes from the same sample — a capability not available in standard RNA cleanup kits:

• Total RNA (>17 nt) — standard protocol recovers all RNA species including mRNA, rRNA, tRNA, and small RNAs
• Large RNA only (>200 nt) — modified protocol selectively retains mRNA and rRNA while small RNAs pass through
• Small RNA only (17–200 nt) — save the column flow-through from the large RNA protocol to collect miRNA, siRNA, and other small RNA species

This selective fractionation capability allows the R1013 to function as both a total RNA cleanup kit and a size-selective RNA fractionation kit — separating small RNA from large RNA in a single workflow when required for downstream applications such as small RNA sequencing or miRNA analysis.

Sample Compatibility — RNA from Any Source The RNA Clean & Concentrator™-5 is compatible with RNA samples from a wide range of sources and preparation methods:

• TRIzol®/chloroform extraction aqueous phase — the most common input for RCC cleanup
• In vitro transcription (IVT) reactions — cleanup of IVT products for mRNA therapeutics, CRISPR guide RNA, and antisense RNA
• DNase I treated RNA — post-DNase cleanup to remove enzyme and buffer components
• Reverse transcription reactions — cleanup and concentration of cDNA following RT
• Enzymatic reactions — removal of enzymes, primers, nucleotides, and salts
• Aqueous RNA samples — concentration of dilute RNA samples into small volumes
• Compatible with ≤0.1% Sarkosyl, ≤0.1% SDS, ≤90% Sucrose, ≤90% Formamide, and ≤2% Formaldehyde

≥6µL Minimum Elution — Highly Concentrated RNA in Minimal Volume The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥6 µl of RNase-free water. The ≥6µL minimum elution volume produces the most highly concentrated RNA eluate available from a spin column cleanup kit — critical for applications requiring maximum RNA concentration in a small volume including single-cell RNA-seq, low-input library preparation, and microinjection. The 10µg binding capacity of the Zymo-Spin™ IC Column allows concentration of dilute RNA samples from large starting volumes into a tiny, highly purified eluate. 

NGS-Ready RNA — A260/A280 and A260/A230 >1.8 Purity — A260/A280 & A260/A230 >1.8. RNA is ready for Next-Gen Sequencing, RT-qPCR, etc. The consistently high purity of RNA purified with the RNA Clean & Concentrator™-5 — confirmed by A260/A280 and A260/A230 ratios consistently above 1.8 — meets the purity standards required for next-generation sequencing library preparation, ensuring that downstream sequencing results are not compromised by organic solvent carryover or protein contamination. 

Single-Buffer System — Simpler and Faster Than Precipitation The unique single-buffer system of the RNA Clean & Concentrator™-5 requires only one binding buffer — eliminating the multiple buffer optimization steps required by precipitation-based RNA cleanup methods. The single RNA Binding Buffer is compatible with the widest possible range of input sample types and compositions without requiring protocol adjustments or buffer substitutions for different sample sources.

Extensively Cited in Published Research — CRISPR, NGS, and Beyond The Zymo Research RNA Clean & Concentrator™-5 is one of the most widely cited RNA cleanup kits in published molecular biology research — referenced in thousands of peer-reviewed publications across RNA biology, CRISPR genome editing, transcriptomics, and NGS library preparation. When a published protocol specifies R1013 or RNA Clean & Concentrator-5, this is the correct product.

Room Temperature Storage — Complete Kit at Bench Temperature Store all kit components (i.e., buffers, columns) at room temperature. All kit components are stored at room temperature — eliminating cold chain requirements and making the R1013 practical for any laboratory environment. Reconstituted DNase I is stored as frozen aliquots after preparation. 

Complete Kit Contents — R1013 (50 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research RNA Clean & Concentrator™-5 R1013 is a comparable alternative to RNA cleanup kits from several major manufacturers:

• Qiagen RNeasy MinElute Cleanup Kit (74204) — 50-prep RNA cleanup and concentration kit
• Thermo Fisher RNA Clean XP (Beckman Coulter A63987) — SPRI bead-based RNA cleanup
• Norgen Biotek RNA Cleanup and Concentration Kit (23600) — 50-prep RNA cleanup kit
• New England Biolabs Monarch RNA Cleanup Kit (T2030L) — RNA cleanup and concentration

These products may differ in protocol, yield, and performance characteristics. The R1013 is specifically cited in published literature and protocols by catalog number.

Key Features

• Zymo Research RNA Clean & Concentrator™-5 — Catalog # R1013
• 50 preparations with Zymo-Spin™ IC Columns
• DNase I Set (E1010) included — in-column DNA elimination
• 5-minute cleanup procedure
• Single-buffer system — simple, rapid, reliable
• Selective recovery of total RNA (>17 nt), large RNA (>200 nt), or small RNA (17–200 nt)
• 10µg RNA binding capacity per column
• Minimum elution volume: ≥6µL
• A260/A280 and A260/A230 >1.8 — NGS-ready purity
• Compatible with TRIzol® aqueous phase, IVT products, DNase-treated RNA, and enzymatic reactions
• RT-PCR-ready, NGS-ready, hybridization-ready
• Room temperature storage — all kit components
• 1-year kit component integrity guarantee
• Lot-to-lot tested for performance and reliability
• Fisher Scientific catalog # 50-444-565
• 200-prep version: R1014
• Without DNase versions: R1015 (50 prep), R1016 (200 prep)

Supported Applications RNA cleanup and concentration following TRIzol®/phenol-chloroform extraction, in vitro transcription (IVT) product cleanup for CRISPR guide RNA, mRNA therapeutics and antisense RNA, post-DNase treatment RNA cleanup, cDNA cleanup following reverse transcription, dilute RNA concentration, small RNA and miRNA cleanup, RNA cleanup prior to NGS library preparation, RT-qPCR sample preparation, microarray hybridization, Northern blotting, RNA size fractionation (large vs. small RNA separation), and any molecular biology workflow requiring rapid, highly concentrated, DNA-free RNA with NGS-grade purity.

Technical Specifications