Zymo Research RNA Clean & Concentrator™-5 R1015 | 50 Preps, Without DNase I, Zymo-Spin™ IC Columns

The Zymo Research RNA Clean & Concentrator™-5 R1015 is a 50-preparation RNA cleanup and concentration kit — providing a simple and reliable method for the rapid preparation of high-quality, RT-PCR-ready RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (>17 nt), large RNAs (>200 nt), and/or small RNAs (17–200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations. DNase I Set is not included — for the DNase I-included version, see R1013 (50 preps) or R1014 (200 preps). 50 preparations. Catalog # R1015. The 200-preparation version without DNase is available as R1016.

R1015 vs. R1013 — Without DNase vs. With DNase: The Critical Distinction The R1015 and R1013 are identical RNA Clean & Concentrator™-5 kits in every way except the DNase I Set:

When to Choose R1015 (Without DNase) Over R1013 (With DNase): The R1015 is the correct choice when DNase treatment is not required — which includes a number of important and common use cases:

• In vitro transcription (IVT) product cleanup — IVT reactions produce RNA from a DNA template, but the DNA template is typically removed by DNase treatment before or after the IVT reaction as a separate step. Using R1015 for IVT RNA cleanup avoids redundant DNase treatment
• cDNA cleanup after reverse transcription — cDNA is DNA, not RNA, and DNase treatment would degrade it. R1015 is the correct kit for cleaning and concentrating first-strand cDNA without DNase exposure
• RNA already confirmed DNA-free — samples that have been previously DNase-treated or sourced from preparations where genomic DNA is absent do not require in-column DNase treatment
• CRISPR guide RNA (sgRNA) cleanup — IVT-produced guide RNAs are typically DNA-free and benefit from R1015 cleanup without DNase
• mRNA therapeutic cleanup — IVT-produced mRNA for therapeutic applications is typically processed through a separate DNase step, making R1015 the preferred cleanup kit
• Any application where DNase could damage the RNA of interest — if the downstream application is sensitive to DNase carryover or if the RNA species of interest is particularly susceptible to nuclease activity

R1015 vs. R1013 vs. R1014 vs. R1016 — Complete Family Overview:

5-Minute RNA Cleanup — Ultra-Pure RNA in Minutes Quick, 5-minute recovery of ultra-pure RNA (≥17 nt) from enzymatic reactions, aqueous phase (following TRIzol® extraction), and other sources. High-quality RNA eluted in ≥6 µl is ready for reverse transcription, microarray, sequencing, etc. The 5-minute preparation time of the RNA Clean & Concentrator™-5 makes it one of the fastest RNA cleanup kits available — processing samples from crude RNA preparation to highly purified, concentrated eluate in minutes.

Selective RNA Recovery — Total RNA, Large RNA, or Small RNA The RNA Clean & Concentrator™-5 allows selective recovery of different RNA size classes from the same sample:

• Total RNA (>17 nt) — standard protocol recovers all RNA species
• Large RNA only (>200 nt) — modified ethanol ratio selectively retains mRNA and rRNA
• Small RNA only (17–200 nt) — save the column flow-through from the large RNA protocol to collect miRNA and siRNA

≥6µL Minimum Elution — Highly Concentrated RNA The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥6 µl of RNase-free water — producing the most highly concentrated RNA eluate available from a spin column cleanup kit, ideal for limited-input applications including single-cell RNA-seq, low-input library preparation, and microinjection.

NGS-Ready RNA — A260/A280 and A260/A230 >1.8 Purity — A260/A280 & A260/A230 >1.8. RNA is ready for Next-Gen Sequencing, RT-qPCR, etc. The consistently high purity of RNA purified with the RNA Clean & Concentrator™-5 meets all NGS library preparation purity standards.

Broad Sample Compatibility Compatible with:

• TRIzol®/chloroform extraction aqueous phase — standard input for RCC cleanup
• In vitro transcription (IVT) products — CRISPR guide RNA, mRNA, antisense RNA
• cDNA following reverse transcription — cleanup and concentration without DNase exposure
• Enzymatic reactions — removal of enzymes, primers, nucleotides, and salts
• Dilute RNA samples — concentration into small, analysis-ready volumes
• Compatible with ≤0.1% Sarkosyl, ≤0.1% SDS, ≤90% Sucrose, ≤90% Formamide, and ≤2% Formaldehyde

Room Temperature Storage — All Kit Components Store all kit components at room temperature. No cold chain required for kit storage.

Complete Kit Contents — R1015 (50 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research RNA Clean & Concentrator™-5 R1015 is a comparable alternative to RNA cleanup kits (without DNase) from several major manufacturers:

• Qiagen RNeasy MinElute Cleanup Kit (74204) — RNA cleanup and concentration kit
• New England Biolabs Monarch RNA Cleanup Kit (T2030L) — RNA cleanup without DNase
• Norgen Biotek RNA Cleanup and Concentration Kit (23600) — RNA cleanup kit
• Thermo Fisher RNA Clean XP (Beckman Coulter A63987) — SPRI bead-based RNA cleanup

These products may differ in protocol, yield, and performance characteristics. The R1015 is specifically cited in published literature and protocols by catalog number.

Key Features

• Zymo Research RNA Clean & Concentrator™-5 — Catalog # R1015
• 50 preparations with Zymo-Spin™ IC Columns
• ⚠️ DNase I Set NOT included — use R1013 for in-column DNase treatment
• 5-minute cleanup procedure
• Single-buffer system — simple, rapid, reliable
• Selective recovery of total RNA (>17 nt), large RNA (>200 nt), or small RNA (17–200 nt)
• 10µg RNA binding capacity per column
• Minimum elution volume: ≥6µL
• A260/A280 and A260/A230 >1.8 — NGS-ready purity
• Ideal for IVT product cleanup, cDNA cleanup, pre-DNased RNA, and any RNA source not requiring DNase treatment
• Compatible with TRIzol® aqueous phase, IVT products, cDNA, enzymatic reactions
• RT-PCR-ready, NGS-ready, hybridization-ready
• Room temperature storage — all kit components
• 1-year kit component integrity guarantee
• Lot-to-lot tested for performance and reliability
• With-DNase version: R1013 (50 prep), R1014 (200 prep)
• 200-prep version without DNase: R1016

Supported Applications IVT product cleanup (CRISPR guide RNA, mRNA, antisense RNA, ribozymes), cDNA cleanup and concentration following reverse transcription, RNA cleanup of pre-DNased samples, RNA cleanup and concentration following TRIzol®/phenol-chloroform extraction (when DNA removal is not required), dilute RNA concentration, small RNA and miRNA cleanup, RNA cleanup prior to NGS library preparation, RT-qPCR sample preparation, mRNA therapeutic production cleanup, microarray hybridization, and any molecular biology workflow requiring rapid, highly concentrated RNA with NGS-grade purity where DNase treatment is not required.

Technical Specifications