Product Description
LIGHT LABS is proud to offer the Zymo Research ZR BAC DNA Miniprep Kit (Cat # D4048) — a streamlined, spin-column-based kit for the efficient, rapid isolation of endotoxin-free BAC (bacterial artificial chromosome) plasmid DNA and other large plasmids such as PACs from E. coli cultures. Combining a modified alkaline lysis protocol with Zymo Research's innovative Zymo-Spin™ IC-XL large-format spin column technology, the D4048 delivers high-yield, high-purity BAC DNA up to approximately 200 kb without the need for chloroform, phenol extraction, or messy precipitation steps.
Zymo Research's signature color-coded reagent system (P1 Red, P2 Green, P3 Yellow) provides easy visual confirmation of complete bacterial cell lysis and neutralization at each step — minimizing errors and making the kit equally accessible to novice and experienced researchers. The Zymo-Spin™ IC-XL column design features zero buffer retention and allows for low-volume elution (≥ 10 µl), yielding highly concentrated, endotoxin-free BAC DNA ideal for sequencing, PCR, endonuclease digestion, transfection, transformation, and in vitro transcription. LIGHT LABS is your trusted source for Zymo Research products.
KEY FEATURES
- Purpose-built for isolation of BAC and PAC plasmid DNA from E. coli — optimized for large constructs up to ~200 kb
- Modified alkaline lysis protocol with color-coded buffers for easy, error-free visual assessment of complete bacterial lysis and neutralization
- Innovative Zymo-Spin™ IC-XL column — zero buffer retention, large-format capacity optimized for high-yield large plasmid recovery
- Low-volume elution (≥ 10 µl) for highly concentrated endotoxin-free DNA eluates
- No chloroform, phenol, or messy precipitation required — clean, safe, simple workflow
- Endotoxin-free purification — suitable for sensitive downstream applications including transfection and in vivo work
- Purified DNA ideal for sequencing, PCR, endonuclease digestion, transfection, transformation, and in vitro transcription
- Fisher Scientific Catalog No. 50-444-257
- Genesee Scientific Catalog No. 11-309Z
- Stored at room temperature (P3 Buffer stored at 0–4°C after opening)
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | D4048 |
| Fisher Scientific Catalog No. | 50-444-257 |
| Genesee Scientific Catalog No. | 11-309Z |
| Brand | Zymo Research |
| Kit Name | ZR BAC DNA Miniprep Kit |
| Number of Preps | 25 |
| DNA Type | BAC/PAC and large plasmid DNA |
| Maximum DNA Size | ~200 kb |
| Column Type | Zymo-Spin™ IC-XL |
| Lysis Method | Modified alkaline lysis with color-coded buffers |
| Minimum Elution Volume | ≥ 10 µl |
| Endotoxin-Free | Yes |
| Organic Solvents Required | No (no chloroform or phenol) |
| Storage Temperature | Room temp (P3 Buffer: 0–4°C after opening) |
| Kit Components | P1 Buffer/Red (10 mL), P2 Buffer/Green (10 mL), P3 Buffer/Yellow (12 mL), Endo-Wash Buffer (6 mL), Plasmid Wash Buffer concentrate (6 mL), DNA Elution Buffer (1 mL), Zymo-Spin™ IC-XL Columns (25), Collection Tubes (50), Instruction Manual |
APPLICATIONS
- BAC (bacterial artificial chromosome) DNA isolation and purification
- PAC (P1-derived artificial chromosome) DNA purification
- Large plasmid DNA purification from E. coli (up to ~200 kb)
- Cosmid DNA purification
- BAC DNA preparation for Sanger and next-generation sequencing
- BAC/PAC DNA preparation for endonuclease restriction mapping
- Large construct DNA preparation for transfection and transgenic animal work
- PCR template preparation from large plasmid constructs
- In vitro transcription from BAC/large plasmid templates
ORDERING INFORMATION
| Cat # | Description | Preps |
|---|---|---|
| D4048 | ZR BAC DNA Miniprep Kit | 25 |
| D4049 | ZR BAC DNA Miniprep Kit | 100 |
| D4036 | Zyppy™ Plasmid Miniprep Kit | 50 |
| D4019 | Zyppy™ Plasmid Miniprep Kit | 100 |
| D4020 | Zyppy™ Plasmid Miniprep Kit | 400 |
| D4025 | Zyppy™ Plasmid Midiprep Kit | 25 |
| D4026 | Zyppy™ Plasmid Midiprep Kit | 50 |
| D4027 | Zyppy™ Plasmid Maxiprep Kit | 10 |
| D4028 | Zyppy™ Plasmid Maxiprep Kit | 20 |
