CELLTREAT® 229105 | 6-Well Tissue Culture Plate with Lid, Flat Bottom, TC-Treated, Sterile, Individually Packaged — 50/Case

The CELLTREAT® 229105 is a 6-well tissue culture treated flat-bottom plate with lid — sterile, clear polystyrene, individually packaged, and built with the full suite of CELLTREAT premium design features including the single-direction raised-rim nesting lid, alpha-numeric well identification, stacking lid feature, and lot number and expiration date printed on every plate. Tissue culture treated surface improves cell adhesion. Plates are labeled with alpha-numeric markings for easy identification of individual wells. Each well has a raised rim which nests into the rings on the single direction lid to reduce evaporation and limit the risk of cross contamination. Lids have a stacking feature for added stability when handling tall stacks of plates.  9.60cm² growth area per well. Maximum well volume: 17mL. Working volume: 1.9–2.9mL. Manufactured from medical grade virgin polystyrene — RNase-free, DNase-free, and non-pyrogenic. Every case ships with a comprehensive CELLTREAT Quality Certificate. 50 plates per case, individually packaged.

229105 vs. 229106 — Choosing the Right 6-Well Pack Size: The 229105 and 229106 are identical 6-well TC-treated flat-bottom plates with the same design, construction, and performance — differing only in case quantity:

The 229105 is the ideal choice for labs with lower 6-well plate consumption, labs evaluating CELLTREAT plates for the first time, or any workflow where the full 100-plate case of the 229106 would result in excess inventory. At 50 plates per case, the 229105 provides the same premium plate quality in a smaller, more manageable quantity.

The 6-Well Format — Maximum Growth Area per Well in the Standard Multi-Well Format The 6-well plate provides the largest individual well growth area (9.60cm²) of any standard multi-well format — making the 229105 the ideal choice for applications requiring substantial cell mass, generous media volumes, or large-diameter well dimensions for imaging. The 6-well format is specifically suited for wound healing and scratch assays, primary cell isolation and expansion, protein extraction for Western blotting, RNA extraction for RNA-seq and qRT-PCR, ChIP and ChIP-seq, virus propagation and harvest, and any downstream application requiring more total cell material than smaller well formats can provide.

TC-Treated Surface — Consistent, Uniform Cell Attachment Across All 6 Wells The tissue culture surface treatment used for CELLTREAT products produces a negatively charged, hydrophilic, and consistent cell attachment surface. Growth surfaces are flat, of uniform thickness and free from striation to maximize usable growth area and transparency. The uniformity of the TC treatment process is tightly controlled across all 6 wells — ensuring equivalent cell attachment kinetics and growth rates in every position on the plate, critical for multi-condition experiments and comparative assays.

Optically Clear Polystyrene — Direct Microscopic Observation and Imaging The clear polystyrene construction allows direct observation of cell morphology, confluency, and health under an inverted microscope without removing the plate from the incubator. Growth surfaces are flat, of uniform thickness and free from striation to maximize usable growth area and transparency. The flat-bottom well geometry provides a uniform focal plane across the entire 9.60cm² well bottom — essential for scratch assay imaging, colony counting, and any live-cell imaging application requiring a large, optically clear, flat growth surface.

Single-Direction Lid with Raised Rim Nesting Rings — Reduced Evaporation and Cross-Contamination Each well has a raised rim which nestles into the rings on the single direction lid to reduce evaporation and limit the risk of cross contamination. The lid also features an exterior stacking ring for added stability when handling tall stacks of plates. The single-direction lid design ensures only one correct orientation — preventing accidental mis-orientation and maintaining the precise well-to-lid ring alignment required for the anti-evaporation and anti-contamination features to function correctly. In the 6-well format where working volumes of 1.9–2.9mL per well must be maintained consistently throughout multi-day experiments, evaporation control is critical for preventing osmolality shifts that affect cell behavior and assay outcomes.

CELLTREAT Quality Certificate — Comprehensive Per-Case Documentation CELLTREAT Scientific Products include a comprehensive "Quality Certificate" with every case of product. The CELLTREAT "Quality Certificate" includes the Part Number, Lot Number, Sterility Assurance Level and Dosage, Endotoxin/Pyrogen Free Assurance, Non-Cytotoxicity Assurance and RNase/DNase Free Assurance. This comprehensive per-case Quality Certificate provides the documentation required for regulated lab environments, GMP compliance, and any quality management system that requires written evidence of consumable certification for each lot used in cell culture workflows.

Class 100,000 Cleanroom — USP Class VI Virgin Polystyrene — ISO 9001:2000 CELLTREAT Tissue Culture Plates are manufactured under the most stringent conditions. Class 100,000 clean room, USP class VI virgin polystyrene, and ISO 9001:2000 quality management systems all contribute to the consistent tissue and cell culture performance. Every 229105 is manufactured in a certified Class 100,000 cleanroom from USP Class VI virgin polystyrene under an ISO 9001:2000 quality management system — the highest manufacturing standards available for tissue culture plasticware.

Alpha-Numeric Well Markings and Stacking Lid Feature Each well is labeled with large alpha-numeric markings for easy identification. A through B rows and 1 through 3 columns (A1–B3) are clearly marked on both the plate base and the lid. The lid stacking ring provides positive mechanical registration between stacked plates — preventing sliding, tipping, and plate separation during incubation and transport.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229105 is a direct compatible replacement for 6-well tissue culture plates from several major manufacturers:

• Thermo Fisher Nunc™ 140675 — Nunc 6-well flat bottom plate with lid, sterile
• Greiner Bio-One 657160 — 6-well TC-treated flat bottom plate with lid, sterile
• Sarstedt 83.3920 — 6-well cell culture plate with lid, TC-treated, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete CELLTREAT® Tissue Culture Treated Multi-Well Plate Family:

Key Features

• 6-well flat-bottom tissue culture treated plate with lid
• TC-treated surface — negatively charged hydrophilic cell attachment surface
• Flat, uniform-thickness, striation-free growth surface — maximizes usable area and transparency
• 9.60cm² growth area per well — maximum standard multi-well format growth area
• Maximum well volume: 17mL / Working volume: 1.9–2.9mL
• Optically clear polystyrene — direct microscopic observation and imaging
• Single-direction lid — one correct orientation only
• Raised rim wells nest into lid rings — reduces evaporation and cross-contamination
• Exterior stacking ring on lid — stable multi-plate incubator stacking
• Large alpha-numeric well labels A1–B3 on plate and lid
• CELLTREAT Quality Certificate included with every case — lot number, SAL, endotoxin/pyrogen-free, non-cytotoxic, RNase/DNase-free assurance
• Lot number and expiration date printed on every plate
• Class 100,000 cleanroom manufactured
• USP Class VI virgin polystyrene
• ISO 9001:2000 quality management system
• Manufactured from medical grade virgin polystyrene
• RNase-free and DNase-free — certified
• Non-pyrogenic
• Sterile — gamma irradiation
• 50 plates per case
• Higher case quantity available as 229106 (100/case)

Supported Applications Wound healing and scratch assays, colony formation and clonogenic assays, primary cell isolation and expansion, large-scale protein extraction for Western blotting and Co-IP, RNA extraction for RNA-seq, qRT-PCR, and Northern blotting, ChIP and ChIP-seq, virus propagation and harvest, conditioned media collection, secretome and cytokine analysis, cell transfection and stable line selection, flow cytometry preparation, large-scale cell expansion before downstream applications, and any mammalian cell culture application requiring the largest individual well growth area in the standard multi-well plate format in a 50-plate case quantity.

Technical Specifications