CELLTREAT® 229148 | 48-Well Tissue Culture Plate with Lid, Flat Bottom, TC-Treated, Sterile, Individually Packaged — 100/Case

Product Description

The CELLTREAT® 229148 is a 48-well tissue culture treated flat-bottom plate with lid — sterile, clear polystyrene, individually packaged, and built with the full suite of CELLTREAT premium design features including the single-direction raised-rim nesting lid, alpha-numeric well identification, stacking lid feature, and lot number and expiration date printed on every plate. Tissue culture treated surface improves cell adhesion. Each well has a raised rim which nests into the rings on the single-direction lid to reduce evaporation and limit the risk of cross-contamination. Lids have a stacking feature for added stability when handling tall stacks of plates. 0.84cm² growth area per well. Maximum well volume: 1.55mL. Working volume: 0.19–0.29mL. Manufactured from medical grade virgin polystyrene — RNase-free, DNase-free, and non-pyrogenic. Every case ships with a comprehensive CELLTREAT Quality Certificate. 100 plates per case, individually packaged.

229147 vs. 229148 — Choosing the Right 48-Well Pack Size: The 229147 and 229148 are identical 48-well TC-treated flat-bottom plates with the same design, construction, and performance — differing only in case quantity:

The 229148 is the preferred choice for high-volume labs that routinely use 48-well plates and want the lowest per-plate cost available in the CELLTREAT 48-well format. At 100 plates per case, the 229148 provides the same premium plate quality as the 229147 at the most economical case price per plate.

The 48-Well Format — The Ideal Middle Ground Between 24-Well and 96-Well The 48-well plate occupies a critical and versatile position between the generous well volume of the 24-well format and the high throughput of the 96-well format. At 0.84cm² per well — more than twice the growth area of a 96-well plate (0.33cm²/well) — the 229148 delivers meaningfully more cell mass per condition while offering nearly twice the number of conditions compared to a 24-well plate. This makes it the preferred format for dose-response studies, multi-condition experiments requiring moderate cell harvest, primary cell culture across many conditions simultaneously, and any workflow where the 96-well format yields insufficient cells per well for quantitative downstream analysis.

TC-Treated Surface — Consistent, Uniform Cell Attachment Across All 48 Wells The tissue culture treatment of the 229148 creates a negatively charged hydrophilic, flat, uniform-thickness, and striation-free surface across the bottom of every well — promoting consistent attachment and spreading of anchorage-dependent cells. The uniformity of the TC treatment is tightly controlled across all 48 wells — ensuring equivalent cell attachment kinetics and growth rates in every position, critical for dose-response studies and multi-condition experiments where well-to-well consistency determines data quality and curve accuracy.

Optically Clear Polystyrene — Direct Microscopic Observation The clear polystyrene construction allows direct observation of cell morphology, confluency, and health under an inverted microscope without removing the plate from the incubator. The flat-bottom well geometry provides a uniform focal plane across the entire 0.84cm² well bottom for consistent microscopic imaging and live-cell monitoring across all 48 wells.

Single-Direction Lid with Raised Rim Nesting Rings — Reduced Evaporation and Cross-Contamination Each well has a raised rim which nests into the rings on the single-direction lid — forming a well-specific physical registration interface that significantly reduces evaporation from individual wells and limits aerosol cross-contamination between adjacent wells. The single-direction lid ensures only one correct orientation — preventing accidental mis-orientation and maintaining the precise well-to-lid ring alignment that is essential in the 48-well format where working volumes of 0.19–0.29mL per well are relatively small and evaporation losses are proportionally significant.

0.84cm² Per Well — More Cell Mass Than 96-Well, More Conditions Than 24-Well The 0.84cm² growth area per well provides more than twice the growth area of a standard 96-well plate (0.33cm²/well) — delivering approximately 2.5× more cells per well at confluence. This additional cell mass per condition bridges the gap between the high-throughput but low-yield 96-well format and the high-yield but lower-throughput 24-well format.

CELLTREAT Quality Certificate — Comprehensive Per-Case Documentation Every case of CELLTREAT 229148 ships with a comprehensive Quality Certificate that includes the Part Number, Lot Number, Sterility Assurance Level and Dosage, Endotoxin/Pyrogen Free Assurance, Non-Cytotoxicity Assurance, and RNase/DNase Free Assurance — supporting regulated lab environments, GMP compliance, and quality management system documentation requirements.

Class 100,000 Cleanroom — USP Class VI Virgin Polystyrene — ISO 9001:2000 Every 229148 is manufactured in a certified Class 100,000 cleanroom from USP Class VI virgin polystyrene under an ISO 9001:2000 quality management system.

Alpha-Numeric Well Markings and Stacking Lid Feature Every plate is molded with clear alpha-numeric well labels (A through F rows, 1 through 8 columns — A1 through F8) on both the plate base and the lid. The lid stacking ring provides positive mechanical registration between stacked plates — preventing sliding, tipping, and plate separation in the incubator and during transport.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229148 is a direct compatible replacement for 48-well tissue culture plates from several major manufacturers:

• Thermo Fisher Nunc™ 150687 — Nunc 48-well flat bottom plate with lid, sterile
• Greiner Bio-One 677180 — 48-well TC-treated flat bottom plate with lid, sterile
• Sarstedt 83.3923 — 48-well cell culture plate with lid, TC-treated, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete CELLTREAT® Tissue Culture Treated Multi-Well Plate Family:

Key Features

• 48-well flat-bottom tissue culture treated plate with lid
• TC-treated surface — consistent, uniform negatively charged hydrophilic cell attachment
• Flat, uniform-thickness, striation-free growth surface
• 0.84cm² growth area per well — more than 2× the growth area of 96-well plates
• Maximum well volume: 1.55mL / Working volume: 0.19–0.29mL
• Optically clear polystyrene — direct microscopic observation and imaging
• Single-direction lid — one correct orientation only
• Raised rim wells nest into lid rings — reduces evaporation and cross-contamination
• Stacking ring on lid — stable multi-plate incubator stacking
• Alpha-numeric well labels A1–F8 on plate and lid
• CELLTREAT Quality Certificate included with every case
• Lot number and expiration date printed on every plate
• Class 100,000 cleanroom manufactured
• USP Class VI virgin polystyrene
• ISO 9001:2000 quality management system
• Manufactured from medical grade virgin polystyrene
• RNase-free and DNase-free — certified
• Non-pyrogenic
• Non-cytotoxic — certified
• Sterile — gamma irradiation
• Individually packaged
• 100 plates per case
• Smaller case quantity available as 229147 (50/case)

Supported Applications Dose-response and IC&sub5;&sub0; studies requiring more cell mass per well than 96-well format provides, multi-condition cytotoxicity assays, primary cell culture across many conditions, gene expression studies by qRT-PCR requiring sufficient cell harvest, protein extraction for Western blotting at moderate scale, transfection efficiency studies across multiple conditions, virus infection assays at multiple MOI, compound screening at low-to-medium throughput, cell-based reporter assays, flow cytometry preparation, and any mammalian cell culture application requiring more growth area than the 96-well format but greater throughput than the 24-well format, in a high-volume 100-plate case.

Technical Specifications