Product Description
The CELLTREAT® 229197 is a 96-well tissue culture treated flat-bottom plate with lid — sterile, clear polystyrene, bulk packed in sleeves of 5, and built with the full suite of CELLTREAT premium design features including the single-direction raised-rim nesting lid, alpha-numeric well identification, stacking lid feature, and lot number printed on every sleeve. Treated tissue culture plates have a hydrophilic surface that is ideal for cell attachment. Alphanumeric markings for easy identification of individual wells. Raised rim on each well nests into the rings on the single direction lid to reduce evaporation and limit the risk of cross-contamination. Stacking ring on the lid offers added stability when handling tall stacks of plates. 0.33cm² growth area per well. Maximum well volume: 0.39mL. Working volume: 0.075–0.20mL. Manufactured from medical grade virgin polystyrene — RNase-free, DNase-free, and non-pyrogenic. Every case ships with a comprehensive CELLTREAT Quality Certificate. 100 plates per case, bulk packed 5 plates per sleeve.
229197 vs. 229196 — Bulk Sleeve vs. Individual Packaging: The 229197 and 229196 are identical 96-well TC-treated flat-bottom plates with the same design, construction, and performance — differing only in packaging format:
| 229197 | 229196 | |
|---|---|---|
| Wells | 96 | 96 |
| Bottom | Flat | Flat |
| TC Treated | Yes | Yes |
| Growth Area/Well | 0.33cm² | 0.33cm² |
| Max Volume/Well | 0.39mL | 0.39mL |
| Working Volume | 0.075–0.20mL | 0.075–0.20mL |
| Packaging | Bulk — 5 plates/sleeve | Individual pack |
| Case Quantity | 100 | 100 |
| Best For | High-volume labs, maximum efficiency | Standard use, sterility assurance |
The 229197 bulk sleeve format is the preferred choice for high-volume labs that consume 96-well plates rapidly and prioritize efficient case opening and plate access over individual sterile packaging. Labs running automated liquid handling platforms, high-throughput screening workflows, or continuous large-volume cell culture routinely prefer bulk sleeve packaging for its speed and reduced packaging waste compared to individually packaged formats.
TC-Treated Surface — Consistent, Uniform Cell Attachment Across All 96 Wells The tissue culture surface treatment used for CELLTREAT products produces a negatively charged, hydrophilic and consistent cell attachment surface. Growth surfaces are flat, of uniform thickness and free from striation to maximize usable growth area and transparency. The uniformity of the TC treatment process is tightly controlled across all 96 wells — ensuring equivalent cell attachment kinetics and growth rates in every position on the plate, critical for quantitative assays where well-to-well consistency determines data quality and dose-response curve accuracy.
Optically Clear Polystyrene — Microscopic Observation and Plate Reader Compatible Clear polystyrene construction lets you easily view samples. The clear polystyrene construction allows direct observation of cells under an inverted microscope and full compatibility with all standard microplate readers for absorbance, fluorescence, and luminescence detection. The flat-bottom well geometry provides a uniform focal plane for consistent microscopic imaging and accurate optical measurements throughout the well area.
Single-Direction Lid with Raised Rim Nesting Rings — Reduced Evaporation and Cross-Contamination Raised rim on each well nests into the rings on the single direction lid to reduce evaporation and limit the risk of cross-contamination. Stacking ring on the lid offers added stability when handling tall stacks of plates. The raised-rim nesting lid design is especially valuable in high-volume 96-well workflows where plates may be stacked and stored for extended periods — the single-direction lid ensures consistent orientation and the nesting rings maintain the anti-evaporation seal throughout multi-day experiments.
Bulk Sleeve Packaging — Maximum Efficiency for High-Volume Labs Not individually packaged. 5 plates/sleeve. The bulk sleeve format of the 229197 provides five plates per sleeve in paper/plastic peel pouches — maintaining plate sterility and physical protection during transit while dramatically reducing the time required to access individual plates compared to opening 100 individual blister packs. For high-throughput labs that open plates in batches of 5 or more at a time, the sleeve format is significantly more efficient. The lot number is printed on each sleeve for traceability.
CELLTREAT Quality Certificate — Comprehensive Per-Case Documentation Every case of CELLTREAT 229197 ships with a comprehensive Quality Certificate that includes the Part Number, Lot Number, Sterility Assurance Level and Dosage, Endotoxin/Pyrogen Free Assurance, Non-Cytotoxicity Assurance, and RNase/DNase Free Assurance — providing the documentation required for regulated lab environments, GMP compliance, and any quality management system requiring written evidence of consumable certification for each lot.
Class 100,000 Cleanroom — USP Class VI Virgin Polystyrene — ISO 9001:2000 CELLTREAT Tissue Cell Culture Plates are manufactured under the most stringent conditions to assure the integrity of tissue and cell culture work. Class 100,000 clean room, USP class VI virgin polystyrene and ISO 9001:2000 quality management systems all contribute to the consistent tissue and cell culture performance.
Compatible Replacement — Cross-Reference Guide
The CELLTREAT® 229197 is a direct compatible replacement for bulk-packaged 96-well tissue culture plates from several major manufacturers:
- Thermo Fisher Nunc™ 167008 — Nunc 96-well flat bottom plate with lid, sterile
- Greiner Bio-One 655180 — 96-well TC-treated flat bottom plate with lid, sterile
- Sarstedt 83.3924 — 96-well cell culture plate with lid, TC-treated, sterile
Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.
Complete CELLTREAT® Tissue Culture Treated Multi-Well Plate Family:
| Cat. # | Wells | Growth Area/Well | Max Volume/Well | Working Volume | Packaging | Case Qty |
|---|---|---|---|---|---|---|
| 229105 | 6-well | 9.60cm² | 17mL | 1.9–2.9mL | Individual | 50 |
| 229106 | 6-well | 9.60cm² | 17mL | 1.9–2.9mL | Individual | 100 |
| 229111 | 12-well | 3.85cm² | 6.80mL | 0.76–1.14mL | Individual | 50 |
| 229112 | 12-well | 3.85cm² | 6.80mL | 0.76–1.14mL | Individual | 100 |
| 229123 | 24-well | 1.93cm² | 3.50mL | 0.38–0.57mL | Individual | 50 |
| 229124 | 24-well | 1.93cm² | 3.50mL | 0.38–0.57mL | Individual | 100 |
| 229147 | 48-well | 0.84cm² | 1.55mL | 0.19–0.29mL | Individual | 50 |
| 229196 | 96-well | 0.33cm² | 0.39mL | 0.075–0.20mL | Individual | 100 |
| 229197 | 96-well | 0.33cm² | 0.39mL | 0.075–0.20mL | Bulk 5/sleeve | 100 |
Key Features
- 96-well flat-bottom tissue culture treated plate with lid
- TC-treated surface — consistent, uniform negatively charged hydrophilic cell attachment
- Flat, uniform-thickness, striation-free growth surface
- 0.33cm² growth area per well
- Maximum well volume: 0.39mL / Working volume: 0.075–0.20mL
- ANSI/SBS standard footprint — compatible with all plate readers and liquid handling systems
- Optically clear polystyrene — microscopy and plate reader compatible
- Single-direction lid — one correct orientation only
- Raised rim wells nest into lid rings — reduces evaporation and cross-contamination
- Stacking ring on lid — stable multi-plate incubator stacking
- Alpha-numeric well labels A1–H12 on plate and lid
- Bulk packed — 5 plates per sleeve, 100 plates per case
- Lot number printed on each sleeve
- CELLTREAT Quality Certificate included with every case
- Class 100,000 cleanroom manufactured
- USP Class VI virgin polystyrene
- ISO 9001:2000 quality management system
- Manufactured from medical grade virgin polystyrene
- RNase-free and DNase-free — certified
- Non-pyrogenic
- Non-cytotoxic — certified
- Sterile — gamma irradiation
- 100 plates per case — bulk sleeve packaging
Supported Applications High-throughput cell viability and cytotoxicity assays (MTT, MTS, WST-1, CellTiter-Glo), high-volume cell proliferation assays, large-scale dose-response studies, automated liquid handling platforms, high-throughput screening, transfection and reporter gene assays, ELISA (cell-based), immunofluorescence and high-content imaging, compound screening, viral infection and propagation, and any high-volume 96-well mammalian cell culture application where bulk sleeve packaging improves workflow efficiency.
Technical Specifications
| Specification | Value |
|---|---|
| SKU / Part Number | 229197 |
| Manufacturer | CELLTREAT Scientific Products |
| Wells | 96 |
| Well Bottom | Flat — uniform thickness, striation-free |
| Growth Area per Well | 0.33cm² |
| Total Growth Area | 31.7cm² |
| Maximum Well Volume | 0.39mL |
| Working Volume | 0.075–0.20mL |
| Surface Treatment | TC-treated — negatively charged hydrophilic |
| Material | USP Class VI medical grade virgin polystyrene |
| Footprint | ANSI/SBS standard |
| Cleanroom Class | Class 100,000 |
| Quality System | ISO 9001:2000 |
| Lid Type | Single-direction, raised rim nesting rings |
| Stacking Feature | Yes — lid stacking ring |
| Well Identification | Alpha-numeric (A1–H12) on plate and lid |
| Quality Certificate | Included — lot #, SAL, endotoxin/pyrogen-free, non-cytotoxic, RNase/DNase-free |
| Lot Printing | Yes — printed on each sleeve |
| Packaging | Bulk — 5 plates per sleeve |
| Sterility | Sterile — gamma irradiation |
| RNase-Free | Yes — certified |
| DNase-Free | Yes — certified |
| Non-Pyrogenic | Yes |
| Non-Cytotoxic | Yes — certified |
| Individual Pkg Option | 229196 (individually packaged) |
| Case Quantity | 100 plates |