CELLTREAT® 229190 | 96-Well Tissue Culture Plate with Lid, Round Bottom, TC-Treated, Sterile, Individually Packaged — 100/Case

Product Description

The CELLTREAT® 229190 is a 96-well tissue culture treated round-bottom plate with lid — sterile, clear polystyrene, individually packaged, and the only round-bottom configuration in the CELLTREAT TC-treated multi-well plate lineup. Each well has a raised rim which nests into the rings on the single direction lid to reduce evaporation and limit the risk of cross contamination. Lids have a stacking feature for added stability when handling tall stacks of plates. 0.33cm² cell growth area per well. TC-treated surface for consistent cell attachment. Manufactured from medical grade virgin polystyrene — RNase-free, DNase-free, and non-pyrogenic. Every case ships with a comprehensive CELLTREAT Quality Certificate. 100 plates per case, individually packaged in paper/plastic peel pouches with lot number printed on each pouch.

Round Bottom vs. Flat Bottom — Choosing the Right 96-Well Format: The single most important distinction when ordering the 229190 is that it features a round-bottom (U-bottom) well geometry — fundamentally different from the flat-bottom 229196 and 229197. Round-bottom and flat-bottom wells are not interchangeable. Choosing the correct geometry for your application is critical:

When to Choose the Round-Bottom 229190: The round-bottom well geometry of the 229190 is specifically designed for applications where cells settle to the lowest point of the well — the center — rather than distributing across a flat surface. This creates a highly concentrated, compact cell pellet or aggregate in a minimal volume that is ideal for:

• Suspension cell culture — non-adherent cell lines (Jurkat, K562, HL-60, Raji, etc.) settle to the round bottom and are easily resuspended or collected by gentle pipetting
• PBMC and immune cell assays — peripheral blood mononuclear cells, lymphocytes, and other immune cells are routinely cultured in round-bottom plates for proliferation assays, cytokine production assays, and mixed lymphocyte reactions
• 3D spheroid formation — the curved well geometry promotes cellular aggregation and self-assembly of 3D tumor spheroids and organoids, particularly when combined with low-attachment surface treatment
• Cell settling and sedimentation assays — assays requiring gravitational cell settling to a defined point for subsequent microscopy or imaging of aggregated cells
• Centrifugation-based cell collection — round-bottom wells facilitate efficient cell pellet formation during plate-based centrifugation for wash steps, staining protocols, and ELISA-based secretion assays
• Miniaturized agglutination assays — hemagglutination, latex agglutination, and other pattern-based agglutination assays that rely on the visual appearance of the cell or particle pattern at the well bottom

TC-Treated Surface — Consistent Cell Attachment When Required The TC-treated surface of the 229190 is applied to the round-bottom surface — providing a negatively charged hydrophilic cell attachment surface for applications where some degree of cell adhesion to the well surface is desired even in a round-bottom format. For applications requiring strict non-adherence (e.g., spheroid formation), a non-treated round-bottom plate may be more appropriate. The TC-treated surface of the 229190 makes it suitable for mixed adherent/suspension cell culture applications and for assays where gentle, controllable adhesion to the curved well surface is beneficial.

Single-Direction Lid with Raised Rim Nesting Rings — Reduced Evaporation and Cross-Contamination Each well has a raised rim which nests into the rings on the single direction lid to reduce evaporation and limit the risk of cross-contamination. Lids have a stacking ring for added stability when handling tall stacks of plates. The single-direction lid design ensures only one correct orientation — particularly important in 96-well round-bottom applications where precise lid-to-well alignment helps protect suspension cell cultures from evaporation-driven concentration changes and aerosol cross-contamination between adjacent wells during extended incubation.

Individual Packaging — Sterile Paper/Plastic Peel Pouches All multiple well plates are individually packaged sterile (gamma irradiated) in paper/plastic peel pouches designed to keep the plates in pristine condition during transit. Lot number is printed on each pouch. The lot number printed on each individual pouch supports full lot-level traceability for regulated applications — allowing the specific lot used for each experiment to be documented without relying solely on the case-level Quality Certificate.

CELLTREAT Quality Certificate — Comprehensive Per-Case Documentation Every case of CELLTREAT 229190 ships with a comprehensive Quality Certificate that includes the Part Number, Lot Number, Sterility Assurance Level and Dosage, Endotoxin/Pyrogen Free Assurance, Non-Cytotoxicity Assurance, and RNase/DNase Free Assurance.

Class 100,000 Cleanroom — USP Class VI Virgin Polystyrene — ISO 9001:2000 Every 229190 is manufactured in a certified Class 100,000 cleanroom from USP Class VI virgin polystyrene under an ISO 9001:2000 quality management system.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229190 is a direct compatible replacement for 96-well round-bottom tissue culture plates from several major manufacturers:

• Thermo Fisher Nunc™ 163320 — Nunc 96-well round bottom plate with lid, sterile
• Greiner Bio-One 650180 — 96-well TC-treated round bottom plate with lid, sterile
• Sarstedt 83.3925 — 96-well cell culture plate round bottom with lid, TC-treated, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete CELLTREAT® Tissue Culture Treated Multi-Well Plate Family:

Key Features

• 96-well round-bottom (U-bottom) tissue culture treated plate with lid
• Round bottom — cells settle to center of well for compact pellet formation
• TC-treated surface — hydrophilic cell attachment surface on curved well bottom
• 0.33cm² growth area per well
• Maximum well volume: 0.39mL
• Clear polystyrene construction
• Single-direction lid — one correct orientation only
• Raised rim wells nest into lid rings — reduces evaporation and cross-contamination
• Stacking ring on lid — stable multi-plate incubator stacking
• Alpha-numeric well labels A1–H12 on plate and lid
• CELLTREAT Quality Certificate included with every case
• Lot number printed on each individual paper/plastic peel pouch
• Class 100,000 cleanroom manufactured
• USP Class VI virgin polystyrene
• ISO 9001:2000 quality management system
• Manufactured from medical grade virgin polystyrene
• RNase-free and DNase-free — certified
• Non-pyrogenic
• Non-cytotoxic — certified
• Sterile — gamma irradiation
• Individually packaged in paper/plastic peel pouches
• 100 plates per case

Supported Applications Suspension cell culture (Jurkat, K562, HL-60, Raji, and other non-adherent cell lines), PBMC culture, T cell and B cell proliferation assays, mixed lymphocyte reactions (MLR), natural killer (NK) cell cytotoxicity assays, cytokine production assays, 3D tumor spheroid formation and culture, organoid aggregation, cell settling and sedimentation assays, plate-based centrifugation wash and staining protocols, flow cytometry sample preparation, hemagglutination and agglutination assays, immune cell functional assays, and any 96-well application requiring compact cell pellet formation or suspension cell culture in a round-bottom well format.

Technical Specifications