CELLTREAT® 229112 | 12-Well Tissue Culture Plate with Lid, Flat Bottom, TC-Treated, Sterile, Individually Packaged — 100/Case

Product Description

The CELLTREAT® 229112 is a 12-well tissue culture treated flat-bottom plate with lid — sterile, clear polystyrene, individually packaged, and built with the full suite of CELLTREAT premium design features including the single-direction raised-rim nesting lid, alpha-numeric well identification, stacking lid feature, and lot number and expiration date printed on every plate. Tissue culture treated surface improves cell adhesion. Each well has a raised rim which nests into the rings on the single-direction lid to reduce evaporation and limit the risk of cross-contamination. Lids have a stacking feature for added stability when handling tall stacks of plates. 3.85cm² growth area per well. Maximum well volume: 6.80mL. Working volume: 0.76–1.14mL. Manufactured from medical grade virgin polystyrene — RNase-free, DNase-free, and non-pyrogenic. Every case ships with a comprehensive CELLTREAT Quality Certificate. 100 plates per case, individually packaged.

The 12-Well Format — The Ideal Middle Ground Between 6-Well and 24-Well The 12-well plate occupies the critical middle position in the multi-well plate format hierarchy — providing significantly more growth area per well than the 24-well format (3.85cm² vs. 1.93cm²) while maintaining twice the throughput of the 6-well format (12 conditions vs. 6). This makes the 229112 the preferred format for experiments that require more cell mass than the 24-well format can provide but benefit from more conditions per plate than the 6-well format allows. The 12-well format is especially well-suited for transfection and gene expression studies requiring moderate cell harvest, infection experiments with multiple MOI conditions, primary cell culture where isolation yields are limited, toxicity studies requiring multiple dose points with sufficient cell mass for Western blotting, and any experiment that would fill a full 6-well plate with fewer conditions than needed.

TC-Treated Surface — Consistent, Uniform Cell Attachment Across All 12 Wells The tissue culture treatment of the 229112 creates a negatively charged hydrophilic, flat, uniform-thickness, and striation-free surface across the bottom of every well — promoting consistent attachment and spreading of anchorage-dependent cells. The uniformity of the TC treatment process is tightly controlled across all 12 wells, ensuring equivalent cell attachment kinetics and growth rates in every position on the plate — critical for comparative multi-condition experiments and any workflow where well-to-well consistency directly affects data quality and experimental reproducibility.

Optically Clear Polystyrene — Direct Microscopic Observation and Imaging The clear polystyrene construction allows direct observation of cell morphology, confluency, and health under an inverted microscope without removing the plate from the incubator — enabling routine monitoring and documentation without disturbing cultures. The flat-bottom well geometry provides a uniform focal plane across the entire 3.85cm² well bottom — suitable for live-cell imaging, immunofluorescence, and scratch assay imaging at the 12-well scale.

Single-Direction Lid with Raised Rim Nesting Rings — Reduced Evaporation and Cross-Contamination Each well has a raised rim which nests into the rings on the single-direction lid — forming a well-specific physical registration interface that significantly reduces evaporation from individual wells and limits aerosol cross-contamination between adjacent wells during routine plate handling. The single-direction lid design ensures only one correct orientation — preventing accidental mis-orientation and maintaining the precise well-to-lid ring alignment required for the anti-evaporation and anti-contamination features to function correctly. In the 12-well format where working volumes of 0.76–1.14mL per well must be maintained consistently throughout multi-day experiments, evaporation control is essential for preventing osmolality shifts that affect cell behavior.

3.85cm² Per Well — Generous Growth Area for the 12-Well Format The 3.85cm² growth area per well of the 229112 is approximately double the growth area of a 24-well plate (1.93cm²/well) — providing enough cell mass for Western blotting, qRT-PCR, and flow cytometry preparation while maintaining the 12-condition throughput that makes the format so versatile. The maximum well volume of 6.80mL and working volume of 0.76–1.14mL provide generous media buffering capacity for metabolically active cells during extended multi-day culture experiments.

CELLTREAT Quality Certificate — Comprehensive Per-Case Documentation Every case of CELLTREAT 229112 ships with a comprehensive Quality Certificate that includes the Part Number, Lot Number, Sterility Assurance Level and Dosage, Endotoxin/Pyrogen Free Assurance, Non-Cytotoxicity Assurance, and RNase/DNase Free Assurance — providing the documentation required for regulated lab environments, GMP compliance, and any quality management system that requires written evidence of consumable certification for each lot.

Class 100,000 Cleanroom — USP Class VI Virgin Polystyrene — ISO 9001:2000 Every 229112 is manufactured in a certified Class 100,000 cleanroom from USP Class VI virgin polystyrene under an ISO 9001:2000 quality management system — the highest manufacturing standards available for tissue culture plasticware.

Stacking Lid Feature and Alpha-Numeric Well Markings Lids have a stacking feature for added stability when handling tall stacks of plates — preventing sliding, tipping, and plate separation in the incubator and during transport. Every plate is molded with clear alpha-numeric well labels (A through C rows, 1 through 4 columns — A1 through C4) on both the plate base and the lid for instant well identification during pipetting, imaging, and data recording.

Lot Number and Expiration Date Printed on Every Plate Every 229112 plate is printed with the lot number and expiration date — supporting full traceability and GMP documentation requirements.

229111 vs. 229112 — Choosing the Right 12-Well Pack Size:

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229112 is a direct compatible replacement for 12-well tissue culture plates from several major manufacturers:

• Thermo Fisher Nunc™ 150628 — Nunc 12-well flat bottom plate with lid, sterile
• Greiner Bio-One 665180 — 12-well TC-treated flat bottom plate with lid, sterile
• Sarstedt 83.3921 — 12-well cell culture plate with lid, TC-treated, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete CELLTREAT® Tissue Culture Treated Multi-Well Plate Family:

Key Features

• 12-well flat-bottom tissue culture treated plate with lid
• TC-treated surface — consistent, uniform negatively charged hydrophilic cell attachment
• Flat, uniform-thickness, striation-free growth surface
• 3.85cm² growth area per well
• Maximum well volume: 6.80mL / Working volume: 0.76–1.14mL
• Optically clear polystyrene — direct microscopic observation and imaging
• Single-direction lid — one correct orientation only
• Raised rim wells nest into lid rings — reduces evaporation and cross-contamination
• Stacking lid feature — stable multi-plate incubator stacking
• Alpha-numeric well labels A1–C4 on plate and lid
• CELLTREAT Quality Certificate included with every case
• Lot number and expiration date printed on every plate
• Class 100,000 cleanroom manufactured
• USP Class VI virgin polystyrene
• ISO 9001:2000 quality management system
• Manufactured from medical grade virgin polystyrene
• RNase-free and DNase-free — certified
• Non-pyrogenic
• Non-cytotoxic — certified
• Sterile — gamma irradiation
• 100 plates per case
• Smaller case quantity available as 229111 (50/case)

Supported Applications Transfection and gene expression studies requiring moderate cell harvest, infection experiments with multiple MOI conditions, primary cell culture at moderate scale, toxicity and viability assays requiring multiple dose points, protein extraction for Western blotting at the 12-well scale, RNA extraction for qRT-PCR and gene expression analysis, immunofluorescence and live-cell imaging, scratch/wound healing assays, virus propagation at intermediate scale, cell-based reporter assays, flow cytometry preparation, and any mammalian cell culture application requiring more growth area than the 24-well format but greater throughput than the 6-well format.

Technical Specifications