CELLTREAT® 229147 | 48-Well Tissue Culture Plate with Lid, Flat Bottom, TC-Treated, Sterile, Individually Packaged — 50/Case

Product Description

The CELLTREAT® 229147 is a 48-well tissue culture treated flat-bottom plate with lid — sterile, clear polystyrene, individually packaged, and built with the full suite of CELLTREAT premium design features including the single-direction raised-rim nesting lid, alpha-numeric well identification, stacking lid feature, and lot number and expiration date printed on every plate. Tissue culture treated surface improves cell adhesion. Plates are labeled with alpha-numeric markings for easy identification of individual wells. Each well has a raised rim which nests into the rings on the single direction lid to reduce evaporation and limit the risk of cross contamination. Lids have a stacking ring for added stability when handling tall stacks of plates. Manufactured from medical grade virgin polystyrene. RNase/DNase Free and Non-pyrogenic. 0.84cm² growth area per well. Maximum well volume: 1.55mL. Working volume: 0.19–0.29mL. Every case ships with a comprehensive CELLTREAT Quality Certificate. 50 plates per case, individually packaged.

The 48-Well Format — The Ideal Middle Ground Between 24-Well and 96-Well The 48-well plate is one of the most versatile yet often overlooked formats in cell culture — occupying the critical space between the generous well volume of the 24-well format and the high throughput of the 96-well format. At 0.84cm² per well, the 48-well format provides more than twice the growth area of a 96-well plate (0.33cm²/well) — delivering meaningfully more cell mass per condition for downstream analysis — while offering nearly twice the number of conditions compared to a 24-well plate. This makes the 229147 the preferred format for:

• Dose-response studies where 5–8 concentrations plus controls fit neatly on a single plate without requiring a 96-well format that provides insufficient cells per well for Western blotting or flow cytometry
• Multi-condition experiments where 24-well plates don't provide enough wells and 96-well plates don't provide enough cells per well
• RNA and protein extraction requiring more material than a 96-well well provides but from more conditions than a 24-well plate accommodates
• Compound screening at low to medium throughput where well-to-well variability in 96-well plates is a concern and larger well volumes are preferred
• Primary cell culture where limited cell numbers must be distributed across many conditions simultaneously

TC-Treated Surface — Consistent, Uniform Cell Attachment Across All 48 Wells The tissue culture treatment of the 229147 creates a negatively charged hydrophilic, flat, uniform-thickness, and striation-free surface across the bottom of every well — promoting consistent attachment and spreading of anchorage-dependent cells. Treated tissue culture plates have a hydrophilic surface that is ideal for cell attachment. The uniformity of the TC treatment process is tightly controlled across all 48 wells, ensuring equivalent cell attachment kinetics and growth rates in every position — critical for dose-response studies and multi-condition experiments where well-to-well consistency directly determines data quality and dose-response curve accuracy.

Optically Clear Polystyrene — Direct Microscopic Observation The clear polystyrene construction allows direct observation of cell morphology, confluency, and health under an inverted microscope without removing the plate from the incubator — enabling routine monitoring and documentation without disturbing cultures. The flat-bottom well geometry provides a uniform focal plane across the entire 0.84cm² well bottom for consistent microscopic imaging and live-cell monitoring across all 48 wells.

Single-Direction Lid with Raised Rim Nesting Rings — Reduced Evaporation and Cross-Contamination Raised rim on each well nests into the rings on the single direction lid to reduce evaporation and limit the risk of cross-contamination. Stacking ring on the lid offers added stability when handling tall stacks of plates. The single-direction lid design ensures only one correct orientation — preventing accidental mis-orientation and maintaining the precise well-to-lid ring alignment that significantly reduces individual-well evaporation and limits aerosol cross-contamination between adjacent wells during routine plate handling. In the 48-well format where working volumes of 0.19–0.29mL per well are relatively small, evaporation control is especially critical for preventing osmolality shifts and volume losses that affect cell behavior across multi-day experiments.

0.84cm² Per Well — More Cell Mass Than 96-Well, More Conditions Than 24-Well The 0.84cm² growth area per well of the 229147 is more than twice the growth area of a standard 96-well plate (0.33cm²/well) — providing approximately 2.5× more cells per well at confluence. This additional cell mass bridges the gap between the high-throughput but low-yield 96-well format and the high-yield but lower-throughput 24-well format — making the 48-well the right choice for any experiment where the 96-well format yields insufficient cells per well for downstream quantitative analysis while the 24-well format provides too few experimental conditions.

CELLTREAT Quality Certificate — Comprehensive Per-Case Documentation Every case of CELLTREAT 229147 ships with a comprehensive Quality Certificate that includes the Part Number, Lot Number, Sterility Assurance Level and Dosage, Endotoxin/Pyrogen Free Assurance, Non-Cytotoxicity Assurance, and RNase/DNase Free Assurance — providing the documentation required for regulated lab environments, GMP compliance, and any quality management system requiring written evidence of consumable certification for each lot.

Class 100,000 Cleanroom — USP Class VI Virgin Polystyrene — ISO 9001:2000 Every 229147 is manufactured in a certified Class 100,000 cleanroom from USP Class VI virgin polystyrene under an ISO 9001:2000 quality management system — the highest manufacturing standards available for tissue culture plasticware.

Alpha-Numeric Well Markings and Stacking Lid Feature Every plate is molded with clear alpha-numeric well labels (A through F rows, 1 through 8 columns — A1 through F8) on both the plate base and the lid — providing instant well identification during pipetting, imaging, and data recording. The lid stacking ring provides positive mechanical registration between stacked plates — preventing sliding, tipping, and plate separation in the incubator and during transport.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229147 is a direct compatible replacement for 48-well tissue culture plates from several major manufacturers:

• Thermo Fisher Nunc™ 150687 — Nunc 48-well flat bottom plate with lid, sterile
• Greiner Bio-One 677180 — 48-well TC-treated flat bottom plate with lid, sterile
• Sarstedt 83.3923 — 48-well cell culture plate with lid, TC-treated, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete CELLTREAT® Tissue Culture Treated Multi-Well Plate Family:

Key Features

• 48-well flat-bottom tissue culture treated plate with lid
• TC-treated surface — consistent, uniform negatively charged hydrophilic cell attachment
• Flat, uniform-thickness, striation-free growth surface
• 0.84cm² growth area per well — more than 2× the growth area of 96-well plates
• Maximum well volume: 1.55mL / Working volume: 0.19–0.29mL
• Optically clear polystyrene — direct microscopic observation and imaging
• Single-direction lid — one correct orientation only
• Raised rim wells nest into lid rings — reduces evaporation and cross-contamination
• Stacking ring on lid — stable multi-plate incubator stacking
• Alpha-numeric well labels A1–F8 on plate and lid
• CELLTREAT Quality Certificate included with every case — lot #, SAL, endotoxin/pyrogen-free, non-cytotoxic, RNase/DNase-free assurance
• Lot number and expiration date printed on every plate
• Class 100,000 cleanroom manufactured
• USP Class VI virgin polystyrene
• ISO 9001:2000 quality management system
• Manufactured from medical grade virgin polystyrene
• RNase-free and DNase-free — certified
• Non-pyrogenic
• Non-cytotoxic — certified
• Sterile — gamma irradiation, peel-to-open tray packaging
• 50 plates per case

Supported Applications Dose-response and IC₅₀ studies requiring more cell mass per well than 96-well format provides, multi-condition cytotoxicity assays, primary cell culture with limited cell numbers across many conditions, gene expression studies by qRT-PCR requiring sufficient cell harvest from multiple conditions, protein extraction for Western blotting at moderate scale, transfection efficiency studies across multiple conditions, virus infection assays at multiple MOI, compound screening at low-to-medium throughput, cell-based reporter assays, and any mammalian cell culture application requiring more growth area than the 96-well format but greater throughput than the 24-well format.

Technical Specifications