Product Description
The CELLTREAT® 229701 is a complete 150mL sterile vacuum filter system — bottle top filter with 0.45µm PES membrane, 150mL solution bottle, and HDPE screw cap — all in one ready-to-use sterile package for vacuum filtration of small volumes of biological solutions, buffers, and media where particle removal and solution clarification are required. 0.45µm PES membrane ideal for solution clarification. The polyethersulfone (PES) membrane provides high flow rate with low protein binding for maximum sample recovery. Vacuum filtration unit includes bottle top filter, 150mL solution bottle, both with graduations, and 50mm screw top cap. Bottles are made of polystyrene and screw top caps of HDPE (high density polyethylene). Sterile by gamma irradiation. RNase-free, DNase-free, and non-pyrogenic. Shelf life: 36 months. 12 filter systems per case, individually packaged.
⚠️ Important — 0.45µm Does NOT Provide Sterile Filtration The 229701 0.45µm filter is designed for solution clarification and particle removal — not sterile filtration. Bacteria range from approximately 0.2µm upward in size, with the smallest bacteria and mycoplasma passing freely through a 0.45µm membrane. For sterile filtration of cell culture media and buffers, the 0.22µm version is the correct product. The 229701 is appropriate for HPLC mobile phase preparation, solution pre-filtration, buffer clarification, and any application where removal of particles ≥0.45µm is the objective without a sterility requirement.
229701 vs. Other CELLTREAT Filter Systems — Choosing the Right Volume and Pore Size:
| Cat. # | Volume | Pore Size | Membrane Dia. | Sterile Filtration | Best For |
|---|---|---|---|---|---|
| 229701 | 150mL | 0.45µm | 50mm | No — clarification | Small-volume clarification, HPLC prep |
| 229702 | 150mL | 0.22µm | 50mm | Yes | Small-volume sterile filtration |
| 229707 | 500mL | 0.22µm | 75mm | Yes | Routine media sterilization |
| 229708 | 1000mL | 0.22µm | 90mm | Yes | Large-volume media sterilization |
0.45µm PES Membrane — High Flow Rate with Low Protein Binding Polyethersulfone (PES) membrane is designed for high flow rate with low binding. The 0.45µm pore size of the 229701 presents significantly less hydraulic resistance than a 0.22µm membrane — allowing faster filtration of equivalent volumes under the same vacuum. The inherently hydrophilic and low protein binding PES surface minimizes loss of proteins, enzymes, and other biologically active components during filtration, making the 229701 well-suited for clarifying protein solutions, enzyme preparations, and biological buffers where sample recovery is important.
150mL Format — Right-Sized for Small-Volume Applications The 150mL format of the 229701 is specifically designed for small-volume filtration workflows — including HPLC mobile phase preparation, small-batch buffer clarification, analytical sample pre-filtration, and any application where a 250mL, 500mL, or 1000mL filter system would result in significant excess unused volume. For labs preparing small volumes of clarified solutions daily, the 150mL format minimizes waste and provides the right capacity for small-scale workflows without the cost inefficiency of using larger filter units.
Easy-to-Read Molded Graduations — Visual Volume Monitoring Easy-to-read molded graduations on bottle top filter. Molded graduation markings on the bottle top filter funnel and solution bottle allow real-time visual monitoring of filtration progress and accurate volume tracking of the filtered solution.
Bold Membrane Identification Printing — 0.45µm Clearly Marked Housings feature boldly printed membrane material and pore size for easy identification. The 0.45µm pore size is boldly printed on the filter housing — critical for preventing accidental use of the 0.45µm clarification filter in applications that require 0.22µm sterile filtration. In labs where both pore sizes are stocked, the bold printed identification eliminates the risk of using the wrong filter.
Angled Hose Barb Adapter — Fits Three Tubing Sizes, Prevents Tipping Angled hose barb adapter fits three size hose diameters and prevents the filter from tipping. The angled hose barb accommodates 6mm, 9mm, and 12mm ID vacuum tubing without adapters, and the angled orientation prevents the filter system from tipping when connected to vacuum tubing.
Curved Bottle Design — Ergonomic Handling Curved bottle design for easy handling. The curved profile of the 150mL solution bottle provides a natural, ergonomic grip for safe handling at the biosafety cabinet and benchtop.
Lot Number and Expiration Date on Every Bottle Top Printed lot number and expiration date on every bottle top. Full lot traceability supporting regulated laboratory environments and quality management documentation requirements.
Complete System — Filter, Bottle, and Cap All Included Filter Systems include bottle top filter and solution bottle with cap. The 229701 is supplied as a complete, ready-to-use system — the bottle top filter, 150mL solution bottle, and HDPE screw-on cap are all included sterile and individually packaged.
Compatible Replacement — Cross-Reference Guide
The CELLTREAT® 229701 is a direct compatible replacement for 150mL 0.45µm PES vacuum filter systems from several major manufacturers:
- Millipore® SCHVU01RE — Stericup 150mL 0.45µm PES filter system, sterile
- Thermo Fisher Nalgene™ 450-0045 — Nalgene Rapid-Flow 150mL 0.45µm PES filter, sterile
- VWR® 10040-438 — VWR 150mL 0.45µm PES bottle top filter system, sterile
Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.
Key Features
- Complete 150mL vacuum filter system — bottle top filter, solution bottle, and HDPE cap included
- 0.45µm PES membrane — particle removal and solution clarification
- ⚠️ 0.45µm does NOT provide sterile filtration — use 229702 (0.22µm, 150mL) for sterile applications
- Hydrophilic PES membrane — high flow rate with low protein binding
- 50mm membrane diameter — optimized for 150mL small-volume filtration
- Improved filter support — sustained flow rates under vacuum
- Easy-to-read molded graduations on bottle top filter and solution bottle
- Bold printed membrane material and 0.45µm pore size — easy identification
- Curved bottle design — ergonomic handling
- Angled hose barb adapter — fits 6mm, 9mm, and 12mm ID tubing, prevents tipping
- Lot number and expiration date printed on every bottle top
- Polystyrene bottle and funnel / HDPE screw cap
- RNase-free and DNase-free — certified
- Non-pyrogenic
- Sterile — gamma irradiation
- Individually packaged
- Shelf life: 36 months
- 12 filter systems per case
Supported Applications HPLC mobile phase preparation and sample pre-filtration, small-volume buffer and solution clarification, biological solution particle removal prior to analysis, pre-filtration before 0.22µm sterile filtration, removal of yeast and large particles from biological solutions, analytical sample preparation, and any laboratory application requiring 0.45µm vacuum filtration of up to 150mL volume.
Technical Specifications
| Specification | Value |
|---|---|
| SKU / Catalog # | 229701 |
| Manufacturer | CELLTREAT Scientific Products |
| Volume | 150mL |
| Pore Size | 0.45µm |
| Sterile Filtration | No — clarification and particle removal only |
| Membrane Material | Polyethersulfone (PES) |
| Membrane Diameter | 50mm |
| Housing Material | Polypropylene |
| Bottle Material | Polystyrene (graduated) |
| Cap Material | HDPE screw-on |
| Hose Barb | Angled — fits 6mm, 9mm, 12mm ID tubing |
| Protein Binding | Low |
| Sterility | Sterile — gamma irradiation |
| RNase-Free | Yes — certified |
| DNase-Free | Yes — certified |
| Non-Pyrogenic | Yes |
| Lot/Expiry Printing | Yes — on every bottle top |
| Packaging | Individually packaged |
| Shelf Life | 36 months |
| Case Quantity | 12 filter systems |