CELLTREAT® 229708 | 1000mL Vacuum Filter System, 0.22µm PES Membrane, Sterile — 12/Case

The CELLTREAT® 229708 is a complete 1000mL sterile vacuum filter system — bottle top filter with upgraded 0.22µm PES membrane, 1000mL solution bottle, and HDPE screw cap — all in one ready-to-use sterile package for routine sterile filtration of large volumes of cell culture media, buffers, and biological solutions. New! Upgraded 0.22µm PES filter membranes now filter up to 43% faster. The polyethersulfone (PES) membrane is the first choice for sterile filtration of cell culture media — combining a 90mm diameter membrane with high flow rate and low protein binding for maximum sample throughput and recovery. Complete vacuum filtration systems include a polystyrene bottletop filter and solution bottle with high-density polyethylene screw-on cap. Angled hose barb adapter fits 6-, 9-, and 12-mm ID tubing and prevents filter from tipping. Sterile by gamma irradiation. RNase-free, DNase-free, and non-pyrogenic. Shelf life: 36 months. 12 filter systems per case, individually packaged.

229707 vs. 229708 — Choosing the Right Volume: The 229707 and 229708 use identical PES membrane technology, pore size, and design features — differing only in volume capacity and membrane diameter:

The 229708 is the preferred choice for labs preparing large volumes of cell culture media, labs running high-throughput culture operations requiring more than 500mL per filtration event, and any workflow where filtering a full liter of media in a single step is more efficient than two 500mL filtrations.

Upgraded 0.22µm PES Membrane — 43% Faster Filtration, 90mm Diameter Upgraded 0.22µm PES filter membranes now filter up to 43% faster. The 229708 features both the upgraded membrane technology and a larger 90mm membrane diameter — the combination of which delivers exceptional filtration throughput for 1000mL volumes. The 90mm membrane provides significantly more filtration surface area than the 75mm membrane of the 229707 — distributing the full 1000mL particulate load across a wider area, reducing local membrane fouling, and maintaining high flow rates throughout the complete filtration volume. Large diameter filter increases flow and prevents clogging. 

0.22µm PES — Sterile Filtration Standard for Cell Culture Media The 0.22µm pore size of the 229708 is the industry standard for sterile filtration — confirmed to remove bacteria, mycoplasma, and other microorganisms ≥0.22µm while passing proteins, growth factors, serum components, and other media constituents. The hydrophilic PES membrane provides fast flow rate with low protein binding — minimizing loss of critical media components during filtration of serum-containing media, supplemented basal media, conditioned media, and other protein-rich biological solutions.

Improved Filter Support — Sustained High Flow Rates at 1000mL Scale Improved filter support provides faster flow rates. The structural support grid beneath the PES membrane maintains membrane geometry under vacuum without deflection or collapse — critical at 1000mL scale where sustained vacuum pressure over a longer filtration time places greater stress on the membrane support structure than smaller-volume filtrations.

Easy-to-Read Molded Graduations — Visual Volume Monitoring Easy-to-read molded graduations on bottle top filter. Molded graduation markings on the bottle top filter funnel allow real-time visual monitoring of filtration progress across the full 1000mL volume — confirming that filtration is proceeding correctly and identifying any flow rate issues early in the filtration run.

Bold Membrane Identification Printing Housings feature boldly printed membrane material and pore size for easy identification. The membrane material (PES) and pore size (0.22µm) are boldly printed on the filter housing — allowing instant identification in labs where multiple CELLTREAT filter sizes and pore sizes are stocked together.

Curved Bottle Design and Angled Hose Barb Curved bottle design for easy handling. Angled hose barb adapter fits three size hose diameters and prevents the filter from tipping. The angled hose barb design is especially important at 1000mL scale — a full 1000mL bottle is significantly heavier than a 500mL bottle, and the angled barb prevents the loaded filter system from tipping under the added weight when connected to vacuum tubing.

Lot Number and Expiration Date on Every Bottle Top Printed lot number and expiration date on every bottle top. Full lot traceability supporting GMP documentation and regulated cell culture quality management requirements.

Complete System — Filter, Bottle, and Cap All Included Filter systems include bottle top filter and solution bottle with cap. The 229708 is supplied as a complete, ready-to-use system — the bottle top filter, 1000mL solution bottle, and HDPE screw-on cap are all included sterile and individually packaged.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229708 is a direct compatible replacement for 1000mL 0.22µm PES vacuum filter systems from several major manufacturers:

• Thermo Fisher Nalgene™ 450-0020 — Nalgene Rapid-Flow 1000mL 0.22µm PES filter system, sterile
• Millipore® SCGPU10RE — Stericup 1000mL 0.22µm PES filter system, sterile
• VWR® 10040-442 — VWR 1000mL 0.22µm PES bottle top filter system, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Key Features

• Complete 1000mL vacuum filter system — bottle top filter, solution bottle, and HDPE cap included
• Upgraded 0.22µm PES membrane — filters up to 43% faster than previous generation
• 90mm membrane diameter — maximum flow area for 1000mL large-volume filtration
• Hydrophilic PES membrane — high flow rate with low protein binding
• Large diameter membrane — reduces clogging during full 1000mL filtration runs
• Improved filter support — sustained high flow rates under vacuum
• Easy-to-read molded graduations on bottle top filter
• Bold printed membrane material and 0.22µm pore size — easy identification
• Curved bottle design — ergonomic handling of large-volume filled bottle
• Angled hose barb adapter — fits 6mm, 9mm, and 12mm ID tubing, prevents tipping
• Lot number and expiration date printed on every bottle top
• Polypropylene housing / polystyrene graduated funnel / HDPE screw cap
• RNase-free and DNase-free — certified
• Non-pyrogenic
• Sterile — gamma irradiation
• Individually packaged
• Shelf life: 36 months
• 12 filter systems per case

Supported Applications Large-volume sterile filtration of cell culture media, high-throughput lab media preparation, FBS and serum filtration at 1000mL scale, large-volume buffer sterilization, preparation of large batches of conditioned media, biological solution sterilization for bioprocessing, and any laboratory application requiring 0.22µm vacuum sterile filtration of up to 1000mL volume.

Technical Specifications