CELLTREAT® 229706 | 250mL Vacuum Filter System, 0.22µm PES Membrane, Sterile — 12/Case

The CELLTREAT® 229706 is a complete 250mL sterile vacuum filter system — bottle top filter with upgraded 0.22µm PES membrane, 250mL solution bottle, and green HDPE screw cap — all in one ready-to-use sterile package for sterile filtration of cell culture media, buffers, and biological solutions at the 250mL scale. New! Upgraded 0.22µm PES filter membranes now filter up to 43% faster. Large diameter filter increases flow and prevents clogging. The 0.22µm PES membrane is the first choice for sterile filtration of cell culture media — removing bacteria, mycoplasma, and other microorganisms ≥0.22µm while providing high flow rate and low protein binding for maximum sample recovery. Complete vacuum filtration systems include a polystyrene bottletop filter and solution bottle with high-density polyethylene screw-on cap. Angled hose barb adapter fits 6-, 9-, and 12-mm ID tubing and prevents filter from tipping. Filter systems are supplied sterile (gamma irradiated); packaged individually. RNase-free, DNase-free, and non-pyrogenic. Shelf life: 36 months. 12 filter systems per case.

250mL Format — The Mid-Volume Sweet Spot for Routine Sterile Filtration The 250mL filter system occupies the critical middle ground between the small-volume 150mL format and the routine-use 500mL format — making the 229706 the preferred choice for labs that regularly prepare 250mL batches of supplemented cell culture media, sterile buffers, and biological solutions without needing the full 500mL capacity. For labs maintaining small numbers of culture vessels, preparing single-bottle media batches, or working with expensive supplements and growth factors where minimizing waste is important, the 250mL format provides exactly the right capacity.

Upgraded 0.22µm PES Membrane — 43% Faster Sterile Filtration Upgraded 0.22µm PES filter membranes now filter up to 43% faster. The upgraded membrane formulation of the 229706 delivers meaningfully faster sterile filtration than previous-generation 0.22µm membranes — reducing the time required to filter a full 250mL volume of cell culture media or biological solutions while maintaining the complete bacterial and mycoplasma retention required for sterile filtration assurance.

75mm Membrane Diameter — Large Diameter Reduces Clogging Large diameter filter increases flow and prevents clogging. The 75mm membrane diameter of the 229706 provides significantly more filtration surface area than the 50mm membranes of the 150mL filter systems — distributing the 250mL particulate load across a wider area, reducing local fouling and clogging, and maintaining higher flow rates throughout the complete filtration volume. This is especially important when filtering serum-containing media, high-protein solutions, or biological fluids with significant particulate content.

0.22µm PES — Sterile Filtration Standard for Cell Culture Media 0.22µm PES membrane is the first choice for sterile filtration of cell culture media. The 0.22µm pore size removes bacteria, mycoplasma, and other microorganisms ≥0.22µm while passing proteins, growth factors, vitamins, and other critical media components. The hydrophilic PES membrane wets instantly and provides low protein binding — minimizing loss of serum proteins, cytokines, enzymes, and growth factors during sterile filtration of supplemented media.

Easy-to-Read Molded Graduations and Bold Pore Size Identification Easy-to-read molded graduations on bottle top filter. Housings feature boldly printed membrane material and pore size for easy identification. The 0.22µm pore size is boldly printed on the filter housing — allowing instant identification at a glance in labs where multiple filter sizes and pore sizes are stocked together.

Green Cap — Easy Visual Identification at a Glance Clear with green cap. The distinctive green HDPE screw-on cap of the 229706 provides an immediate visual identifier that distinguishes the 250mL 0.22µm filter system from other CELLTREAT filter formats on the shelf — reducing the risk of reaching for the wrong filter in a busy biosafety cabinet environment.

Angled Hose Barb Adapter, Curved Bottle Design, and Improved Filter Support Angled hose barb adapter fits three size hose diameters and prevents the filter from tipping. Curved bottle design for easy handling. Improved filter support provides faster flow rates. The angled barb accommodates 6mm, 9mm, and 12mm ID vacuum tubing without adapters and prevents tipping, while the improved support grid sustains high flow rates throughout the complete 250mL filtration volume.

Lot Number and Expiration Date on Every Bottle Top Printed lot number and expiration date on every bottle top. Full lot traceability for regulated cell culture environments and GMP documentation requirements.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229706 is a direct compatible replacement for 250mL 0.22µm PES vacuum filter systems from several major manufacturers:

• Millipore® SCGPU02RE — Stericup 250mL 0.22µm PES filter system, sterile
• Thermo Fisher Nalgene™ 450-0020 — Nalgene Rapid-Flow 250mL 0.22µm PES filter, sterile
• VWR® 10040-438 — VWR 250mL 0.22µm PES bottle top filter system, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete CELLTREAT® Filter System Family — Confirmed:

Key Features

• Complete 250mL vacuum filter system — bottle top filter, solution bottle, and green HDPE cap included
• Upgraded 0.22µm PES membrane — filters up to 43% faster than previous generation
• Sterile filtration — removes bacteria and mycoplasma ≥0.22µm
• 75mm membrane diameter — large diameter reduces clogging vs. 50mm membranes
• Hydrophilic PES membrane — high flow rate with low protein binding
• Improved filter support — sustained flow rates under vacuum
• Easy-to-read molded graduations on bottle top filter
• Bold printed membrane material and 0.22µm pore size — easy identification
• Green HDPE cap — easy visual identification on the shelf
• Curved bottle design — ergonomic handling
• Angled hose barb adapter — fits 6mm, 9mm, and 12mm ID tubing, prevents tipping
• Lot number and expiration date printed on every bottle top
• Polystyrene bottle and funnel / green HDPE screw cap
• RNase-free and DNase-free — certified
• Non-pyrogenic
• Sterile — gamma irradiation
• Individually packaged
• Shelf life: 36 months
• 12 filter systems per case

Supported Applications Sterile filtration of 250mL volumes of cell culture media, preparation of supplemented growth media in mid-size batches, sterile buffer preparation, sterile filtration of specialized cell culture reagents and supplements, enzyme and protein solution sterilization, and any laboratory application requiring 0.22µm vacuum sterile filtration of up to 250mL volume.

Technical Specifications