CELLTREAT® 229702 | 250mL Vacuum Filter System, 0.45µm PES Membrane, Sterile — 12/Case

The CELLTREAT® 229702 is a complete 250mL sterile vacuum filter system — bottle top filter with 0.45µm PES membrane, 250mL solution bottle, and HDPE screw cap — designed for vacuum filtration of 250mL volumes where particle removal, solution clarification, and pre-filtration are required without a full sterile filtration requirement. 0.45µm PES membrane ideal for solution clarification. Large diameter filter increases flow and prevents clogging. The polyethersulfone (PES) membrane provides high flow rate with low protein binding. Complete vacuum filtration systems include a polystyrene bottletop filter and solution bottle with high-density polyethylene screw-on cap. Angled hose barb adapter fits 6-, 9-, and 12-mm ID tubing and prevents filter from tipping. Filter systems are supplied sterile (gamma irradiated); packaged individually. RNase-free, DNase-free, and non-pyrogenic. Shelf life: 36 months. 12 filter systems per case.

⚠️ Important — 0.45µm Does NOT Provide Sterile Filtration The 229702 0.45µm filter is designed for solution clarification and particle removal — not sterile filtration. For sterile filtration of cell culture media and buffers at 250mL scale, the 0.22µm 229706 is the correct product. The 229702 is appropriate for HPLC mobile phase preparation, solution pre-filtration, buffer clarification, and any 250mL application where removal of particles ≥0.45µm is required without a sterility requirement.

229702 vs. 229706 — Choosing the Right Pore Size for 250mL Filtration:

0.45µm PES Membrane — High Flow Rate with Low Protein Binding Polyethersulfone (PES) membrane is designed for high flow rate with low binding. The 0.45µm pore size presents significantly less hydraulic resistance than a 0.22µm membrane — delivering faster filtration of 250mL volumes under the same vacuum. The inherently hydrophilic PES surface provides low protein binding — minimizing loss of proteins and biologically active components during clarification.

75mm Membrane Diameter — Large Diameter Reduces Clogging Large diameter filter increases flow and prevents clogging. The 75mm membrane diameter provides significantly more filtration surface area than smaller-diameter bottle top filters — distributing the 250mL particulate load across a wider area, reducing local fouling, and maintaining high flow rates throughout the complete filtration volume.

Easy-to-Read Molded Graduations and Bold Pore Size Identification Easy-to-read molded graduations on bottle top filter. Housings feature boldly printed membrane material and pore size for easy identification. The 0.45µm pore size is boldly printed on the filter housing — critical in labs where both 229702 (0.45µm) and 229706 (0.22µm) 250mL filters are stocked together.

Angled Hose Barb Adapter, Curved Bottle Design, and Improved Filter Support Angled hose barb adapter fits three size hose diameters and prevents the filter from tipping. Curved bottle design for easy handling. Improved filter support provides faster flow rates. The angled barb accommodates 6mm, 9mm, and 12mm ID vacuum tubing without adapters, prevents tipping, and the improved support grid sustains high flow rates throughout the complete 250mL filtration volume.

Lot Number and Expiration Date on Every Bottle Top Printed lot number and expiration date on every bottle top. Full lot traceability for regulated laboratory environments and quality management documentation.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229702 is a direct compatible replacement for 250mL 0.45µm PES vacuum filter systems from several major manufacturers:

• Millipore® SCHVU02RE — Stericup 250mL 0.45µm PES filter system, sterile
• Thermo Fisher Nalgene™ 450-0045 — Nalgene Rapid-Flow 250mL 0.45µm PES filter, sterile
• VWR® 10040-440 — VWR 250mL 0.45µm PES bottle top filter system, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete CELLTREAT® Filter System Family — Confirmed:

Key Features

• Complete 250mL vacuum filter system — bottle top filter, solution bottle, and HDPE cap included
• 0.45µm PES membrane — particle removal and solution clarification at 250mL scale
• ⚠️ 0.45µm does NOT provide sterile filtration — use 229706 (0.22µm, 250mL) for sterile applications
• 75mm membrane diameter — large diameter reduces clogging
• High flow rate with low protein binding PES membrane
• Improved filter support — sustained flow rates under vacuum
• Easy-to-read molded graduations on bottle top filter
• Bold printed membrane material and 0.45µm pore size — easy identification, prevents mix-up with 229706
• Curved bottle design — ergonomic handling
• Angled hose barb adapter — fits 6mm, 9mm, and 12mm ID tubing, prevents tipping
• Lot number and expiration date printed on every bottle top
• Polystyrene bottle and funnel / HDPE screw cap
• RNase-free and DNase-free — certified
• Non-pyrogenic
• Sterile — gamma irradiation
• Individually packaged
• Shelf life: 36 months
• 12 filter systems per case

Supported Applications HPLC mobile phase preparation and sample pre-filtration at 250mL scale, buffer and solution clarification, biological solution particle removal prior to analysis, pre-filtration before 0.22µm sterile filtration, removal of yeast and large particles from 250mL solutions, and any laboratory application requiring 0.45µm vacuum filtration of up to 250mL volume.

Technical Specifications