CELLTREAT® 229707 | 500mL Vacuum Filter System, 0.22µm PES Membrane, Sterile — 12/Case

The CELLTREAT® 229707 is a complete 500mL sterile vacuum filter system — bottle top filter with upgraded 0.22µm PES membrane, solution bottle, and HDPE screw cap — all in one ready-to-use sterile package for routine sterile filtration of cell culture media, buffers, and biological solutions. New! Upgraded 0.22µm PES filter membranes now filter up to 43% faster. The polyethersulfone (PES) membrane is the first choice for sterile filtration of cell culture media — combining high flow rate with low protein binding for maximum sample recovery. Complete vacuum filtration systems include a polystyrene bottletop filter and solution bottle with high-density polyethylene screw-on cap. Sterile by gamma irradiation. RNase-free, DNase-free, and non-pyrogenic. Shelf life: 36 months. 12 filter systems per case, individually packaged.

Upgraded PES Membrane — 43% Faster Filtration Than Before Upgraded 0.22µm PES filter membranes now filter up to 43% faster. The upgraded PES membrane of the 229707 is a meaningful performance improvement over previous-generation filter membranes — reducing the time required to filter a full 500mL volume of cell culture media, serum-containing solutions, or viscous biological fluids. For labs that filter large volumes of media routinely, faster filtration directly reduces processing time, decreases the risk of membrane clogging before filtration is complete, and improves overall lab throughput.

0.22µm PES Membrane — The Gold Standard for Sterile Filtration The 0.22µm pore size of the 229707 PES membrane is the industry standard for sterile filtration of cell culture media and biological solutions — confirmed to remove bacteria, mycoplasma, and other microorganisms ≥0.22µm while passing viruses, proteins, small molecules, and other components of interest without significant retention. Hydrophilic PES (polyethersulfone) membrane provides fast flow rate. Low protein binding — excellent for filtering solutions for cell culture applications. The hydrophilic PES membrane wets instantly on contact with aqueous solutions without requiring pre-wetting steps — and its inherently low protein binding surface minimizes loss of growth factors, serum proteins, cytokines, and other biologically active components during filtration.

Large Diameter Membrane — Faster Flow, Less Clogging Large diameter filter increases flow and prevents clogging. 500mL filters feature expanded 90mm diameter membrane to increase flow and reduce clogs for faster filtration. The large membrane diameter of the 229707 provides significantly more filtration surface area than smaller-diameter bottle top filters — distributing the particulate load across a wider membrane area, reducing local fouling and clogging, and maintaining higher flow rates throughout the full filtration volume. For challenging samples such as serum-containing media, high-protein solutions, or biological fluids with significant particulate content, the larger membrane diameter makes the difference between a complete filtration and a clogged, partially filtered volume.

Improved Filter Support — Sustained High Flow Rates Improved filter support provides faster flow rates. The structural support grid beneath the PES membrane is engineered to maintain membrane geometry under vacuum without deflection or collapse — allowing the membrane to operate at full flow capacity throughout the filtration run without the flow rate reduction that occurs when membrane support is inadequate and the membrane deflects under vacuum pressure.

Easy-to-Read Molded Graduations — Visual Volume Monitoring During Filtration Easy-to-read molded graduations on bottle top filter. Easy-to-read molded graduation marks on bottle top filter. Molded graduation markings on the bottle top filter funnel allow real-time visual monitoring of filtration progress — tracking how much volume has filtered through without needing to interrupt the process. Unlike printed graduations that fade or wash off, molded graduations are permanent structural features of the polystyrene funnel.

Bold Membrane Identification Printing — No Confusion at the Filter Rack Housings feature boldly printed membrane material and pore size for easy identification. The membrane material (PES) and pore size (0.22µm) are boldly printed directly on the filter housing — allowing instant identification of the correct filter at a glance in busy lab environments where multiple filter types and pore sizes may be stored together. This eliminates the risk of using the wrong pore size for sterile filtration — a critical safety feature in cell culture labs where using a 0.45µm filter instead of a 0.22µm filter would result in a non-sterile filtrate.

Curved Bottle Design — Ergonomic Handling Curved bottle design for easy handling. The curved profile of the 500mL solution bottle provides a natural, ergonomic grip that makes it easier to handle safely — particularly when the bottle is filled with 500mL of media and connected to a vacuum line. The curved design reduces the risk of dropping the bottle during transfer and improves stability during vacuum filtration at the biosafety cabinet.

Angled Hose Barb Adapter — Fits Three Tubing Sizes, Prevents Tipping Angled hose barb adapter fits three size hose diameters and prevents the filter from tipping. Angled hose barb adapter fits 6-, 9-, and 12-mm ID tubing and prevents filter from tipping. The angled hose barb design serves two important functions — it accommodates three standard vacuum tubing sizes (6mm, 9mm, and 12mm ID) without requiring adapters or additional fittings, and the angled orientation prevents the filter system from tipping over when connected to vacuum tubing. Tipping during filtration is one of the most common causes of filtration failure and contamination — the angled barb design eliminates this risk.

Lot Number and Expiration Date on Every Bottle Top — Full Traceability Printed lot number and expiration date on every bottle top. The lot number and expiration date are printed on every bottle top — supporting full lot traceability for regulated cell culture environments, GMP documentation requirements, and any quality management system requiring consumable lot documentation.

Complete System — Filter, Bottle, and Cap All Included Filter Systems include bottle top filter and solution bottle with cap. The 229707 is supplied as a complete, ready-to-use system — no additional components required. The bottle top filter, 500mL solution bottle, and HDPE screw-on cap are all included sterile and individually packaged — allowing immediate use at the biosafety cabinet without assembly of separately ordered components.

Sterile by Gamma Irradiation — Individually Packaged Filter systems are supplied sterile (gamma irradiated); packaged individually. Every 229707 is sterile by gamma irradiation and individually packaged for single-use — maintaining sterility until the moment of use and eliminating any concern about contamination from shared or reused filter components.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229707 is a direct compatible replacement for 500mL 0.22µm PES vacuum filter systems from several major manufacturers:

• Thermo Fisher Nalgene™ 450-0020 — Nalgene Rapid-Flow 500mL 0.22µm PES filter system, sterile
• Millipore® SCGPU05RE — Stericup 500mL 0.22µm PES filter system, sterile
• VWR® 10040-440 — VWR 500mL 0.22µm PES bottle top filter system, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete CELLTREAT® Filter System Family:

Key Features

• Complete 500mL vacuum filter system — bottle top filter, solution bottle, and HDPE cap included
• Upgraded 0.22µm PES membrane — filters up to 43% faster than previous generation
• Hydrophilic PES membrane — high flow rate with low protein binding
• Large diameter membrane — expanded flow area reduces clogging
• Improved filter support — sustained high flow rates under vacuum
• Easy-to-read molded graduations on bottle top filter
• Bold printed membrane material and pore size — easy identification
• Curved bottle design — ergonomic handling
• Angled hose barb adapter — fits 6mm, 9mm, and 12mm ID tubing
• Angled barb prevents filter tipping during vacuum filtration
• Lot number and expiration date printed on every bottle top
• Polypropylene housing / polystyrene graduated funnel / HDPE screw cap
• RNase-free and DNase-free — certified
• Non-pyrogenic
• Sterile — gamma irradiation
• Individually packaged
• Shelf life: 36 months
• 12 filter systems per case

Supported Applications Sterile filtration of cell culture media, FBS and serum filtration, buffer sterilization, sterile filtration of protein and enzyme solutions, preparation of conditioned media, biological solution sterilization prior to storage, and any laboratory application requiring 0.22µm vacuum sterile filtration of up to 500mL volume.

Technical Specifications