CELLTREAT® 229710 | 50mL Centrifuge Tube Filter, 0.22µm PES, Sterile — 12/Case

The CELLTREAT® 229710 is a 50mL centrifuge tube filter with 0.22µm PES membrane — a uniquely practical sterile filtration format that eliminates the sample transfer step by filtering directly into a standard 50mL conical centrifuge tube. Eliminate a sample transfer step by filtering directly into a 50mL tube. Polyethersulfone (PES) membrane for high flow and low binding. The 0.22µm pore size provides full sterile filtration — removing bacteria, mycoplasma, and other microorganisms ≥0.22µm — making the 229710 the sterile filtration version of the 50mL centrifuge tube filter format. One Centrifuge Tube Stand included with each case of 50mL Centrifuge Tube Filter Systems. Centrifuge tube Stands are manufactured from polypropylene and are autoclavable, reusable and recyclable. Centrifuge Tube Stand will hold one 50mL tube, three 15mL tubes and three microcentrifuge tubes (1.5mL). Includes tube filter, centrifuge tube and cap. Sterile by gamma irradiation. RNase-free, DNase-free, and non-pyrogenic. 12 per case.

The Key Advantage — Sterile Filtration Directly Into the Centrifuge Tube Standard bottle top filter systems require two steps for sterile filtration into a centrifuge tube: filter the solution into the collection bottle, then aseptically transfer the sterile filtrate into the centrifuge tube. The CELLTREAT 229710 eliminates the transfer step entirely — the 0.22µm filter unit sits directly on the 50mL centrifuge tube, and the sterile filtrate collects directly in the tube that goes into the centrifuge. This integration of sterile filtration and collection into a single vessel reduces handling steps, minimizes contamination risk from the additional aseptic transfer, and reduces total processing time. For any sterile filtration workflow where the final destination of the filtrate is a 50mL centrifuge tube, the 229710 is the most efficient format.

0.22µm PES Membrane — Full Sterile Filtration in the Tube The 0.22µm pore size of the 229710 is the industry standard for sterile filtration — removing bacteria, mycoplasma, and other microorganisms ≥0.22µm while passing proteins, growth factors, and other biologically active components. The hydrophilic PES membrane provides high flow rate with low protein binding — minimizing loss of critical components during sterile filtration of serum-containing solutions, protein preparations, and supplemented media in small volumes. This is the critical distinction from the 229709 (0.45µm) — the 229710 provides confirmed sterile filtration while the 229709 provides clarification only.

229710 vs. 229709 — Sterile vs. Clarification:

Complete System — Filter, Centrifuge Tube, Cap, and Stand All Included Includes tube filter, centrifuge tube and cap. Every 229710 unit is a complete, ready-to-use sterile system — the filter insert, 50mL conical centrifuge tube, and cap are all included sterile and individually packaged. One Centrifuge Tube Stand included with each case. The included centrifuge tube stand provides stable benchtop support during vacuum filtration — holding the 50mL tube upright and secure while the filter unit is connected to the vacuum line.

Autoclavable, Reusable Centrifuge Tube Stand Centrifuge tube Stands are manufactured from polypropylene and are autoclavable, reusable and recyclable. The included stand holds one 50mL tube, three 15mL tubes and three microcentrifuge tubes (1.5mL) — a versatile benchtop accessory that can be sterilized and reused indefinitely beyond the 229710 filtration workflow.

Sterile by Gamma Irradiation — Individually Packaged Every 229710 is sterile by gamma irradiation and individually packaged — maintaining sterility until the moment of use and ensuring the complete filtration system is aseptic from the moment it is opened at the biosafety cabinet.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229710 is a direct compatible replacement for 50mL centrifuge tube filters with 0.22µm PES from several major manufacturers:

• Thermo Fisher Nalgene™ 190-2520 — Nalgene tube top filter, 50mL, 0.22µm
• VWR® 10218-934 — 50mL tube top filter, 0.22µm PES, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete CELLTREAT® 50mL Centrifuge Tube Filter Family:

Key Features

• 50mL centrifuge tube filter — filter directly into the centrifuge tube, no transfer step
• 0.22µm PES membrane — full sterile filtration, removes bacteria and mycoplasma
• Hydrophilic PES membrane — high flow rate with low protein binding
• Complete system — tube filter, 50mL centrifuge tube, and cap all included
• One autoclavable, reusable polypropylene centrifuge tube stand included per case
• Stand holds 1 × 50mL tube, 3 × 15mL tubes, 3 × 1.5mL microcentrifuge tubes
• Eliminates aseptic transfer step vs. standard bottle top filter systems
• Sterile — gamma irradiation
• Individually packaged
• RNase-free and DNase-free — certified
• Non-pyrogenic
• 12 per case

Supported Applications Sterile filtration of small volumes of cell culture media, sterile buffer preparation in centrifuge tube format, sterile filtration of protein solutions and biological reagents prior to centrifugation, sterile filtration of enzyme preparations, conditioned media sterile filtration, and any application requiring 0.22µm sterile vacuum filtration directly into a 50mL centrifuge tube without an intermediate aseptic transfer step.

Technical Specifications